Recombinant Human RPB8 His Protein

SDS-Page: RPB8 Protein [NBP2-23345]

• Reactivity: Hu
• Applications: PAGE
• Concentration: 0.5 mg/ml

Recombinant Human RPB8 His Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-150 of Human His-tag RPB8
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMAGILFE DIFDVKDIDP EGKKFDRVSR LHCESESFKM DLILDVNIQI YPVDLGDKFR LVIASTLYED GTLDDGEYNP TDDRPSRADQ FEYVMYGKVY RIEGDETSTE AATRLSAYVS YGGLLMRLQG DANNLHGFEV DSRVYLLMKK LAF

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: POLR2H
• Purity: >90%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 19.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 8.0), 0.15 M NaCl, 10% glycerol, 1 mM DTT
• Preservative: No Preservative
• Concentration: 0.5 mg/ml
• Purity: >90%, by SDS-PAGE

Alternate Names for Recombinant Human RPB8 His Protein

• DNA-directed RNA polymerases I, II, and III subunit RPABC3
• hsRPB8
• II, and III 17.1 kDa polypeptide
• II, and III subunit ABC3
• polymerase (RNA) II (DNA directed) polypeptide H
• RPB8 homolog

Background

POLR2H is one of the essential subunits of RNA polymerase II that is shared by the other two eukaryotic DNA-directed RNA polymerases, I and III. Recombinant human POLR2H protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: POLR2H