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RPA2 [p Ser33] Antibody [PE]

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Product Details

Summary
Reactivity Hu, Pm-OrSpecies Glossary
Applications Flow
Clonality
Polyclonal
Host
Rabbit
Conjugate
PE

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NB100-544AF350
Alexa Fluor 405
NB100-544AF405
Alexa Fluor 488
NB100-544AF488
Alexa Fluor 532
NB100-544AF532
Alexa Fluor 594
NB100-544AF594
Alexa Fluor 647
NB100-544AF647
Alexa Fluor 700
NB100-544AF700
Alexa Fluor 750
NB100-544AF750
Biotin
NB100-544B
DyLight 350
NB100-544UV
DyLight 405
NB100-544V
DyLight 488
NB100-544G
DyLight 550
NB100-544R
DyLight 594
NB100-544DL594
DyLight 650
NB100-544C
DyLight 680
NB100-544FR
DyLight 755
NB100-544IR
FITC
NB100-544F
HRP
NB100-544H
Janelia Fluor 549
NB100-544JF549
Janelia Fluor 646
NB100-544JF646
Unconjugated
NB100-544
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RPA2 [p Ser33] Antibody [PE] Summary

Immunogen
Immunogen was a phosphorylated synthetic peptide, which represented a portion of human replication protein A2, 32 kDa surrounding phosphorylated serine that corresponded to position 33 using the numbering given in entry NP_002937.1 (GeneID 6118).
Modification
p Ser33
Specificity
NB100-544 is specific for human RPA-32 pSer33 protein.
Predicted Species
Orangutan (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RPA2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Flow Cytometry 1:10-1:1000
Control
UBE2J2/UBC6 Antibody

Reactivity Notes

Based on 100% sequence identity, this antibody is predicted to react with Orangutan.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for RPA2 [p Ser33] Antibody [PE]

  • REPA2
  • Replication factor A protein 2
  • replication protein A 32 kDa subunit
  • Replication protein A 34 kDa subunit
  • replication protein A2 (32kD)
  • replication protein A2, 32kDa
  • RF-A protein 2
  • RP-A p32
  • RP-A p34
  • RPA2
  • RPA32
  • RPA34

Background

Replication Protein A (RPA) is a single stranded DNA binding protein. Human RPA is a heterotrimeric protein containing subunits of 14, 32 and 70kDa. This protein complex is highly conserved in eukaryotes and is essential in DNA replication, homologous recombination and nucleotide excision repair. RPA is a target for DNA dependent protein kinase (DNA-PK), Ataxia Telangiectasia Mutated gene (ATM) and a cyclin dependent protein kinase. The middle subunit, RPA 32, is phosphorylated during the S-phase of the cell cycle in response to DNA damage, and during apoptosis of Jurkat T-lymphocytes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunohistochemistry-Paraffin: RPA70 Antibody [NB100-2204] - Human ovarian carcinoma. Antibodies: Affinity purified rabbit anti- RPA70 Cat. No.used at a dilution of 1:1,000 (1 ug/ml) (left) and used at a dilution of 1:5,000 (0.2ug/ml (right). Detection: DAB
Western Blot: RPA70 Antibody [NB100-2204] - Detection of Human RPA70 by Western Blot. Samples: Whole cell lysate (5, 15 and 50 ug) from HeLa and (15 and 50 ug) from 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RPA70 antibody NB100-2204 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
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Immunohistochemistry-Paraffin: POLR1A Antibody [NBP2-32016] - Staining of human cerebral cortex shows strong nuclear positivity in neurons.
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(11.1)
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Western Blot: RPA14 Antibody (11.1) [NB100-159] - A. 30 ug A549 whole cell lysate/extract B. 30 ug MCF-7 whole cell lysate/extract 15 % SDS-PAGE RPA14 antibody [11.1] dilution: 1:1000
Immunohistochemistry-Paraffin: RPA14 Antibody (11.1) [NB100-159] - Mahlarvu xenograft. RPA14 antibody [11.1] dilution: 1:200. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Western Blot: RPA14 Antibody (11.1) [NB100-159] - Sample (30 ug of whole cell lysate) A: MCF7 15% SDS PAGE, antibody diluted at 1:500.
(7 Publications)
Species: Hu, Mu
Applications: WB, IHC, IHC-P, IP
ATR Antibody (2B5)
NB100-308
Western Blot: ATR Antibody (2B5) [NB100-308] - Human Raji whole cell extract. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.
Immunocytochemistry/Immunofluorescence: ATR Antibody (2B5) [NB100-308] - Analysis of HeLa, using antibody at 1:100 dilution.
Western Blot: ATR Antibody (2B5) [NB100-308] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ATR antibody [2B5] diluted at 1:500.
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Species: Hu, Mu
Applications: WB, Func, ICC/IF, IP, In vitro, KO
MMAB Antibody
(OTI2G5)
NBP2-03417
Western Blot: MMAB Antibody (2G5) [NBP2-03417] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MMAB (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MMAB.
Immunohistochemistry-Paraffin: MMAB Antibody (2G5) [NBP2-03417] Staining of paraffin-embedded Human lymph node tissue using anti-MMAB mouse monoclonal antibody.
Flow Cytometry: MMAB Antibody (2G5) [NBP2-03417] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-MMAB antibody, and then analyzed by flow cytometry.
Species: Hu
Applications: WB, Flow, IHC, IHC-P
TEM8/ANTXR1
Antibody (200C1339(SB
NB100-56585
Western Blot: TEM8/ANTXR1 Antibody (200C1339(SB20)) [NB100-56585] - Detection in TEM8 transfected cell lysate using this antibody.
Immunohistochemistry-Paraffin: TEM8/ANTXR1 Antibody (200C1339(SB20)) [NB100-56585] - Detection of TEM8/ANTXR1 in human liver cancer section using 5 ug/ml concentration of TEM8 antibody. Uniform weak staining was observed in the cancerous cells, while the blood vessels depicted the expected intense positivity for TEM8.
Western Blot: TEM8/ANTXR1 Antibody (200C1339(SB20)) [NB100-56585] - Analysis of human heart lysates (35ug per lane, RIPA buffer) using TEM8 antibody (NB100-56585) at 0.3ug/ml. Band observed at 65kDa. (Expected MW of 62.8kDa according to NP_115584.1).
(5 Publications)
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
ATM Antibody (2C1)
NB100-309
Western Blot: ATM Antibody (2C1) [NB100-309] - HeLa whole cell and nuclear extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ATM antibody (2C1) [NB100-309] diluted at 1:1000. Theoretical molecular weight 351 kDa.
Immunohistochemistry-Paraffin: ATM Antibody (2C1) [NB100-309] - Human Testis (formalin-fixed, paraffin-embedded) stained with ATM antibody (2C1) [NB100-309] at 5 ug/ml followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Western Blot: ATM Antibody (2C1) [NB100-309] - Detection of human ATM protein using ATM antibody (2C1) [NB100-309] by western blot or immunoprecipitation. Theoretical molecular weight 351 kDa.
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Chk1 Antibody
NB100-464
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Histone H2AX [p
Ser139] Antibody [Unc ...
AF2288
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(3H6)
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p53 Antibody (PAb
240)
NB200-103
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Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready, Flow-IC
PCNA Antibody
(PC10)
NB500-106
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Immunocytochemistry/Immunofluorescence: PCNA Antibody (PC10) [NB500-106] - Development of the germinal zones in the tree shrew neocortex. Immunofluorescence for PCNA (yellow) and DAPI staining (blue) on 30 um-cryosections of E32-P1 tree shrew neocortex. The top margin of the image corresponds to the transition zone SVZ/intermediate zone. Scale bars, 50 um. VZ, ventricular zone; SVZ, subventricular zone; iSVZ, inner SVZ; oSVZ, outer SVZ. Image collected and cropped by CiteAb from the following publication (http://journal.frontiersin.org/article/10.3389/fnana.2018.00029/full), licensed under a CC-BY licence.
Immunohistochemistry: PCNA Antibody (PC10) [NB500-106] - Analysis of PCNA in mouse cornea. Image courtesy of product review by Bo-Yie Chen.
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Nbs1 Antibody
NB100-143
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Western Blot: Nbs1 Antibody [NB100-143] - Western Blot with Nbs1 Antibody [NB100-143]. KSHV LANA recruits MRN (Mre11-Rad50-NBS1) complex. Co-immunoprecipitation of endogenous LANA and MRN proteins in BC3 cells. Cells were lysed using TBS-T buffer and the cell lysate was incubated with benzonase. After centrifugation, supernatant was incubated overnight with anti-LANA or IgG-control beads. The precipitated complexes were analyzed for the presence of endogenous Rad50, Mre11 and NBS1 by SDS-PAGE and immunoblotting. Image collected and cropped by CiteAb from the following publication (http://dx.plos.org/10.1371/journal.ppat.1006335), licensed under a CC-BY licence.
Immunocytochemistry/Immunofluorescence: Nbs1 Antibody [NB100-143] - ICC/IF analysis of Nbs1 in HeLa cells with Nbs1 Antibody [NB100-143]. Image from verified customer review.
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Applications: WB, ChIP, ELISA, Flow, IB, ICC/IF, IHC, IHC-P, IP, ISH, PAGE, KO
Rad51 Antibody
(14B4)
NB100-148
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Immunocytochemistry/Immunofluorescence: Rad51 Antibody (14B4) [NB100-148] - Staining of RAD51 nuclear foci in U2OS cells using RAD51 14B4 antibody. Cells were pre-extracted with CSK buffer before fixation with 4% PFA. RAD51 14B4 was used at 1:1000 dultion. DAPI was used to counterstain the nucleus. Scale bar, 10 mciro-meter.
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(4 Reviews)
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p21/CIP1/CDKN1A
Antibody (WA-1)
NBP2-29463
Immunohistochemistry-Paraffin: p21/CIP1/CDKN1A Antibody (WA-1 (HJ21)) [NBP2-29463] - Formalin-fixed, paraffin-embedded human bladder carcinoma stained with p21 Monoclonal Antibody (WA-1).
(14 Publications)
Species: Hu, Mu, Rt, Pm
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
alpha-Sarcoglycan
Antibody (JA51-81)
NBP2-67150
Western Blot: alpha-Sarcoglycan Antibody (JA51-81) [NBP2-67150] - Analysis of alpha sarcoglycan on rat heart tissue lysates using anti-alpha sarcoglycan antibody at 1:500 dilution.
Immunohistochemistry-Frozen: alpha-Sarcoglycan Antibody (JA51-81) [NBP2-67150] - Human skeletal muscle tissue. IHC-Fr image submitted by a verified customer review.
Immunohistochemistry-Paraffin: alpha-Sarcoglycan Antibody (JA51-81) [NBP2-67150] - Analysis of paraffin-embedded mouse heart tissue using anti-alpha sarcoglycan antibody. Counter stained with hematoxylin.
(1 Review)
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-Fr, IHC-P, IP
NLRP2/NALP2
Antibody
NB100-56155
Western Blot: NLRP2/NALP2 Antibody [NB100-56155] - Analysis of NLRP2/NALP2 using antibody at 1:2000. HEK293T: mock transfected cells, HEK293T-NALP2:NALP2 transiently transfected cells; THP-1: untreated cells; THP1-LPS: cells treated with 1 ug/ml LPS for4h. MCF-7: untreated cells.
Immunohistochemistry-Paraffin: NLRP2/NALP2 Antibody [NB100-56155] - NLRP2 was detected in immersion fixed paraffin-embedded sections of human placenta using Rabbit Anti-Human NLRP2 polyclonal Antibody (Catalog # NB100-56155) at 1:500 for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm in horionic villi.
(1 Publication)
Species: Hu
Applications: WB, IHC, IHC-P, IP
GPHA2 Antibody
NBP3-04883
Western Blot: GPHA2 Antibody [NBP3-04883] - Analysis of extracts of rat brain, using GPHA2 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit.
Species: Hu, Mu, Rt
Applications: WB
ATP6V0A2 Antibody
NBP1-59069
Western Blot: ATP6V0A2 Antibody [NBP1-59069] - HeLa cells, concentration 3.3 ug/ml.
Immunohistochemistry: ATP6V0A2 Antibody [NBP1-59069] - Human kidney lysate tissue at an antibody concentration of 5.0 ug/ml using anti-ATP6V0A2 antibody
Western Blot: ATP6V0A2 Antibody [NBP1-59069] - Hela tissue lysate at a concentration of 1ug/ml.
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
RPA2 [p Ser33]
Antibody [PE]
NB100-544PE
RPA2 [p Ser33] Antibody [PE]
Species: Hu, Pm-Or
Applications: Flow

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Control Lysate(s)

UBE2J2/UBC6 Antibody

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB100-544AF350
Alexa Fluor 405 NB100-544AF405
Alexa Fluor 488 NB100-544AF488
Alexa Fluor 532 NB100-544AF532
Alexa Fluor 594 NB100-544AF594
Alexa Fluor 647 NB100-544AF647
Alexa Fluor 700 NB100-544AF700
Alexa Fluor 750 NB100-544AF750
Biotin NB100-544B
DyLight 350 NB100-544UV
DyLight 405 NB100-544V
DyLight 488 NB100-544G
DyLight 550 NB100-544R
DyLight 594 NB100-544DL594
DyLight 650 NB100-544C
DyLight 680 NB100-544FR
DyLight 755 NB100-544IR
FITC NB100-544F
HRP NB100-544H
Janelia Fluor 549 NB100-544JF549
Janelia Fluor 646 NB100-544JF646
PE NB100-544PE
Learn about Custom Antibody Labeling

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Bioinformatics

Gene Symbol RPA2
Entrez
Uniprot