RPA2 Antibody [Alexa Fluor® 750]

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 750

RPA2 Antibody [Alexa Fluor® 750] Summary

• Immunogen: E. coli-derived recombinant human RPA2
  Leu141-Glu270
  Accession # P15927
• Specificity: Detects human RPA2 in direct ELISAs and Western blots.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for RPA2 Antibody [Alexa Fluor® 750]

• REPA2
• Replication factor A protein 2
• replication protein A 32 kDa subunit
• Replication protein A 34 kDa subunit
• replication protein A2 (32kD)
• replication protein A2, 32kDa
• RF-A protein 2
• RP-A p32
• RP-A p34
• RPA2
• RPA32
• RPA34

Background

RPA2 (replication protein A 32 kDa subunit; also RFA2 and RPA p34) is a 32 kDa DNA‑binding protein that constitutess one of three subunits comprising the PRA heterotrimer complex. In conjunction with 70 kDa RPA1 and 14 kDa RPA3, RPA2 participates in DNA replication, recombination and repair. Human RPA2 is 270 amino acids (aa) in length. It contains a Gly/Ser‑rich N‑terminus (aa 1‑33), a DNA‑binding domain (aa 43‑171) and a protein‑interaction C‑terminus (aa 187‑270). Phosphorylation of the N‑terminus on Ser4/8/23/29/33, plus Thr21, regulates RPA complex interactions with DNA repair and replication complexes. There are multiple splice variants. Three contain N‑terminal extensions: one shows an 88 aa insertion after Ser4, another shows a 12 aa substitution for aa 1‑4, and a third shows a four aa insertion after Ser4. There is also a deletion of aa 93‑98, and a potential truncation after Gln175. Over aa 141‑270, human RPA2 shares 83% aa identity with mouse RPA2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.