ROBO3 Antibody

Western Blot: Robo3 Antibody [NBP1-06071] - Analysis of Robo3 (internal) using this antibody at 0.5 ug/ml in Human Brain (Cerebellum) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Product Discontinued

ROBO3 Antibody Summary

• Immunogen: Peptide with sequence C-RRKQRKELSHYT corresponding to internal region according to NP_071765.2.
• Epitope: C-RRKQRKELSHYT
• Predicted Species: Porcine (100%), Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ROBO3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.5 - 1 ug/ml
• Application Notes: WB: Approx. 150 kDa band observed in human brain (cerebellum and hippocampus) lysates (calculated MW of 148 kDa band according to NP_071765.2).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for ROBO3 Antibody

• FLJ21044
• HGPPS
• HGPS
• RBIG1horizontal gaze palsy with progressive scoliosis
• retinoblastoma inhibiting gene 1
• RIG1
• ROBO3
• roundabout (axon guidance receptor, Drosophila) homolog 3
• roundabout homolog 3
• roundabout, axon guidance receptor, homolog 3 (Drosophila)
• Roundabout-like protein 3

Background

The Robo3 gene is a member of the Roundabout (ROBO) gene family that controls neurite outgrowth, growth cone guidance, andaxon fasciculation. ROBO proteins are a subfamily of the immunoglobulin transmembrane receptor superfamily. SLITproteins 1-3, a family of

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ROBO3 Antibody (NBP1-06071)(1)

Publication using NBP1-06071

• Kato H, Takeuchi O, Sato S et al. Differential roles of MDA5 and RIG-I helicases in the recognition of RNA viruses. Nature 2006-05-04 [PMID: 16625202]

Bioinformatics

• Gene Symbol: ROBO3
• Entrez:
  • 64221(Human)
• Uniprot:
  • NP_071765.2(Human)