Home » RNMTL1 » RNMTL1 Proteins » Recombinant Human RNMTL1 Protein

Recombinant Human RNMTL1 Protein

 Submit a Review

Images

 
(
Enlarge)
SDS-Page: RNMTL1 Recombinant Protein [NBP2-52041] - 15% SDS Page (3 ug)
SDS-Page: RNMTL1 Recombinant Protein [NBP2-52041] - 15% SDS Page (3 ug)

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE
Concentration
1 mg/ml

Order Details

100% Guarantee on every product

Recombinant Human RNMTL1 Protein Summary

Description
A denatured recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-420 of Human RNMTL1

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMAALVRP ARFVVRPLLQ VVQAWDLDAR RWVRALRRSP VKVVFPSGEV VEQKRAPGKQ PRKAPSEASA QEQREKQPLE ESASRAPSTW EESGLRYDKA YPGDRRLSSV MTIVKSRPFR EKQGKILLEG RRLISDALKA GAVPKMFFFS RLEYLKELPV DKLKGVSLIK VKFEDIKDWS DLVTPQGIMG IFAKPDHVKM TYPKTQLQHS LPLLLICDNL RDPGNLGTIL RSAAGAGCSK VLLTKGCVDA WEPKVLRAGM GAHFRMPIIN NLEWETVPNY LPPDTRVYVA DNCGLYAQAE MSNKASDHGW VCDQRVMKFH KYEEEEDVET GASQDWLPHV EVQSYDSDWT EAPAAVVIGG ETYGVSLESL QLAESTGGKR LLIPVVPGVD SLNSAMAASI LLFEGKRQLR GRAEDLSRDR SYH
Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
MRM3
Purity
>85%, by SDS-PAGE

Applications/Dilutions

Theoretical MW
49.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20 mM Tris-HCl buffer (pH 8.0), 10% glycerol
Preservative
No Preservative
Concentration
1 mg/ml
Purity
>85%, by SDS-PAGE

Alternate Names for Recombinant Human RNMTL1 Protein

  • EC 2.1.1
  • EC 2.1.1.-
  • FLJ10581
  • HC90
  • putative RNA methyltransferase
  • RNA methyltransferase like 1
  • RNA methyltransferase-like protein 1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NCOA6 Antibody
NB200-335
Western Blot: NCOA6 Antibody [NB200-335] - Detection of Human ASC2 by Western Blot. Samples: Whole cell lysate (5, 15 and 50 ug) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ASC2 antibody NB200-335 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunohistochemistry-Paraffin: NCOA6 Antibody [NB200-335] - FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-ASC2 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Immunoprecipitation: NCOA6 Antibody [NB200-335] - Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ASC2 antibody used for IP at 6 ug per reaction. ASC2 was also immunoprecipitated by a previous lot of this antibody and rabbit anti-ASC2 antibody. For blotting immunoprecipitated ASC2, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
(7 Publications)
Species: Hu
Applications: WB, IHC, IHC-P, IP
YY1 Antibody
NBP2-20932
Western Blot: YY1 Antibody [NBP2-20932] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with YY1 antibody diluted at 1:2000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunocytochemistry/Immunofluorescence: YY1 Antibody [NBP2-20932] - YY1 antibody detects YY1 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: YY1 stained by YY1 antibody diluted at 1:1000. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar= 10 um.
Immunohistochemistry-Paraffin: YY1 Antibody [NBP2-20932] - Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using antibody at 1:500 dilution.
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
PIMT Antibody
NB100-2595
Western Blot: PIMT Antibody [NB100-2595] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. NB100-2595 used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate (B).
Immunohistochemistry: PIMT Antibody [NB100-2595] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- PIMT used at a dilution of 1:200 (1ug/ml). Detection: DAB
Immunohistochemistry-Paraffin: PIMT Antibody [NB100-2595] - FFPE section of human non-small cell lung cancer. Affinity purified rabbit anti-PIMT used at a dilution of 1:250.
Species: Hu
Applications: WB, IHC, IHC-P, IP
GDNF [Unconjugated]
212-GD/CF
Recombinant Human GDNF (Catalog # 212‑GD/CF) induces SH-SY5Y human neuroblastoma cell proliferation in the presence of Recombinant Human GFR alpha ‑1 Fc Chimera (Catalog # 714‑GR). The activity is approximately 9-fold greater than the top competitor's GDNF.
1 μg/lane of Recombinant Human GDNF was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing single bands at 17 kDa and 34 kDa, respectively.
(104 Publications)
Species: Hu
PCMT1 Antibody
NBP1-85806
Western Blot: PCMT1 Antibody [NBP1-85806] - Analysis in MCF-7 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-PCMT1 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Immunocytochemistry/Immunofluorescence: PCMT1 Antibody [NBP1-85806] - Staining of human cell line U-2 OS shows localization to cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: PCMT1 Antibody [NBP1-85806] - Staining of human stomach shows cytoplasmic positivity in glandular cells.
(1 Publication)
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P, KD
u-Plasminogen
Activator/Urokinase [ ...
1310-SE
Recombinant Human u-Plasminogen Activator/Urokinase (Catalog # 1310-SE) is measured by its ability to cleave a peptide substrate,N-carbobenzyloxy-Gly-Gly-Arg-7-amido-4-methylcoumarin (Z-GGR-AMC).
(8 Publications)
Species: Hu
CREB Antibody
NBP1-90364
Western Blot: CREB Antibody [NBP1-90364] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
Immunocytochemistry/Immunofluorescence: CREB Antibody [NBP1-90364] - Staining of human cell line U-2 OS shows localization to nucleoplasm. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: CREB Antibody [NBP1-90364] - Staining of human small intestine shows strong nuclear positivity in glandular cells.
(3 Reviews)
(1 Publication)
Species: Hu, Mu, Rt
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P
Prion protein
Antibody (SC57-05)
NBP2-67569
Western Blot: Prion protein Antibody (SC57-05) [NBP2-67569] - Analysis of Prion Protein(PrP) on rat brain tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Immunocytochemistry/Immunofluorescence: Prion protein Antibody (SC57-05) [NBP2-67569] - Staining PrP in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: Prion protein Antibody (SC57-05) [NBP2-67569] - Analysis of paraffin-embedded rat brain tissue using anti-PrP antibody. Counter stained with hematoxylin.
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P
ASC2 Antibody
NB100-56076
Western Blot: ASC2 Antibody [NB100-56076] - Used to analyze ASC2/POP1. HEK-293 cells were transiently transfected with plasmids encoding ASC-2, POP1 or a negative control plasmid. ASC-2 and POP1 were detected at the expected 12 kDa molecular weight.
Immunohistochemistry-Paraffin: ASC2 Antibody [NB100-56076] - Used at 1:2000 to analyze ASC-2 expression in mouse spleen. A and A1, spleen at low and high magnification, respectively. ASC-2 staining is seen in the granulocytes, whereas the lymph and megakaryocytes are negative.
Immunohistochemistry: ASC2 Antibody [NB100-56076] - Used at 1:2000 to analyze ASC-2/POP1 expression in mouse. A) prostate tissue from a male mouse prostatitis. B) kidney from a mouse with pyelonephritis. A1 and B1, high magnification of A and B, respectively.
(1 Publication)
Species: Hu, Mu
Applications: WB, IHC, IHC-P, IP
MTH1 Antibody
NB100-109
Knockout Validated: MTH1 Antibody [NB100-109] - Western blot shows lysates of HEK293T human embryonic kidney parental cell line and MTH1 knockout (KO) HEK293T cell line. PVDF membrane was probed with 1:500 of Rabbit Anti-Human MTH1 Polyclonal Antibody (Catalog # NB100-109) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for MTH1 at approximately 18 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in the knockout HEK293T cell line. This experiment was conducted under reducing conditions.
Western Blot: MTH1 Antibody [NB100-109] - Characteristic expression of MTH1 in human gastric cancer tissues and ten digestive tract cancer cell lines. The cells from esophageal cancer cell lines: KYSE-450, EC109 and EC9706, liver cancer cell lines: SMMC-7721, HepG2 and ZIP177 as well as the corresponding normal cell lines: Het-1A, L02 and GES-1 were cultured and lysed. The MTH1 protein levels were determined by Western Blot. GAPDH was used as a loading control. At least three independent experiments were performed for each group. Image collected and cropped by Cite Ab from the following publication (Potent and specific MTH1 inhibitors targeting gastric cancer. Cell Death Dis (2019)) licensed under a CC-BY licence.
Immunocytochemistry/Immunofluorescence: MTH1 Antibody [NB100-109] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-MTH1 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
(28 Publications)
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IB, ICC/IF, IHC, IHC-Fr, IHC-P, KO, Single-Cell Western
Gemin 4 Antibody
NB110-40591
Western Blot: Gemin 4 Antibody [NB110-40591] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. NB110-40591 used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate (B).
Immunohistochemistry-Paraffin: Gemin 4 Antibody [NB110-40591] - Human breast carcinoma. Antibody used at a dilution of 1:200.
Species: Hu
Applications: WB, IHC, IHC-P, IP
GLOD4 Antibody
NBP1-88462
Western Blot: GLOD4 Antibody [NBP1-88462] - Analysis using Anti-GLOD4 antibody NBP1-88462 (A) shows similar pattern to independent antibody NBP1-88463 (B).
Immunocytochemistry/Immunofluorescence: GLOD4 Antibody [NBP1-88462] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.
Immunohistochemistry-Paraffin: GLOD4 Antibody [NBP1-88462] - Staining of human testis.
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
PPAR gamma/NR1C3
Antibody
NBP2-22106
Western Blot: PPAR gamma/NR1C3 Antibody [NBP2-22106] - Total protein from 3T3-L1 mouse embryonic fibroblast adipose-like cell line, separated by 4-12% SDS-PAGE, transferred to nitrocellulose membrane and blocked in 5% non-fat milk for 1h at room temperature. The membrane was probed with anti-PPAR gamma 1:800 in non-fat milk. Lane 2-7 increasing protein concentration, undifferentiated adipocytes. Lane 8-13 increasing protein concentration, differentiated adipocytes. This image was submitted via customer review.
Immunohistochemistry-Paraffin: PPAR gamma Antibody [NBP2-22106] - IHC analysis of PPAR gamma in mouse liver.
Western Blot: PPAR gamma Antibody [NBP2-22106] - Western blot analysis of PPAR gamma in 1. human adipose and 2. human adrenal lysate.
(2 Reviews)
(5 Publications)
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P, IP
Phospholipase C
like 1 Antibody
NBP1-80896
Immunohistochemistry-Paraffin: Phospholipase C like 1 Antibody [NBP1-80896] - Staining of human lung shows weak to moderate cytoplasmic positivity in pneumocytes and in macrophages.
Immunohistochemistry-Paraffin: Phospholipase C like 1 Antibody [NBP1-80896] - Staining of human cerebellum shows moderate cytoplasmic positivity in purkinje cells.
Immunohistochemistry-Paraffin: Phospholipase C like 1 Antibody [NBP1-80896] - Staining of human heart muscle shows moderate cytoplasmic positivity.
Species: Hu
Applications: IHC, IHC-P
MAD1L1/MAD1
Antibody
NBP1-32775
Western Blot: MAD1L1/MAD1 Antibody [NBP1-32775] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MAD1 antibody diluted by 1:5000.
Immunocytochemistry/Immunofluorescence: MAD1L1/MAD1 Antibody [NBP1-32775] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: MAD1 protein stained by MAD1 antibody diluted at 1:500. Red: HEC1, a kinetochore marker, stained by HEC1 antibody [9G3.23] diluted at 1:500. Blue: Hoechst 33342 staining.
Immunohistochemistry-Paraffin: MAD1L1/MAD1 Antibody [NBP1-32775] - Paraffin-embedded SW480 Xenograft, using antibody at 1:100 dilution.
(2 Publications)
Species: Hu, Mu, Rt
Applications: WB, ChIP, ICC/IF, IHC, IHC-P, IP
FTSJ2 Antibody
NBP2-30894
Western Blot: FTSJ2 Antibody [NBP2-30894] - Analysis in control (vector only transfected HEK293T lysate) and FTSJ2 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry-Paraffin: FTSJ2 Antibody [NBP2-30894] - Staining of human heart muscle shows cytoplasmic positivity in myocytes.
Species: Hu
Applications: WB, IHC, IHC-P
TRBP Antibody
NBP2-24725
Western Blot: TRBP Antibody [NBP2-24725] - Analysis of TRBP in human brain lysate in the 1) absence, 2) presence of immunizing peptide using this antibody at 6 ug/ml.
Immunohistochemistry-Paraffin: TRBP Antibody [NBP2-24725] - IHC staining of normal human brain using this antibody at 5 ug/ml.
Immunohistochemistry-Paraffin: TRBP Antibody [NBP2-24725] - IHC staining of normal human brain using control (rabbit Ig) at 5 ug/ml.
(1 Publication)
Species: Hu, Eq, Pm
Applications: WB, IHC, IHC-P
Recombinant Human
RNMTL1 Protein
NBP2-52041
Recombinant Human RNMTL1 Protein
Species: Hu
Applications: PAGE

Publications for RNMTL1 Recombinant Protein (NBP2-52041) (0)

There are no publications for RNMTL1 Recombinant Protein (NBP2-52041).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for RNMTL1 Recombinant Protein (NBP2-52041) (0)

There are no reviews for RNMTL1 Recombinant Protein (NBP2-52041). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for RNMTL1 Recombinant Protein (NBP2-52041) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Other Available Formats

Learn about Custom Antibody Labeling

Additional RNMTL1 Products

Bioinformatics Tool for RNMTL1 Recombinant Protein (NBP2-52041)

Discover related pathways, diseases and genes to RNMTL1 Recombinant Protein (NBP2-52041). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for RNMTL1 Recombinant Protein (NBP2-52041)

Discover more about diseases related to RNMTL1 Recombinant Protein (NBP2-52041).

Blogs on RNMTL1

There are no specific blogs for RNMTL1, but you can read our latest blog posts.

Contact Information

Product PDFs

  PDF Datasheet
  Request CofA
  Request SDS

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Human RNMTL1 Protein and receive a gift card or discount.


Bioinformatics

Gene Symbol MRM3