RNF2 Antibody - BSA Free

Western Blot: RNF2 Antibody [NBP1-49966] - Detection of Human and Mouse RNF2 by Western Blot (h&m) and Immunoprecipitation (h). Samples: Whole cell lysate from K562 (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg), and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-RNF2 antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. RNF2 was also immunoprecipitated by rabbit anti-RNF2 antibody, which recognizes a downstream epitope. For blotting immunoprecipitated RNF2 was used. Detection: Chemiluminescence with exposure times of 30 seconds (A and B).

Immunohistochemistry-Paraffin: RNF2 Antibody [NBP1-49966] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-RNF2 used at a dilution of 1:1,000 (1ug/ml). Detection: Vector ImmPACT NovaRED

Immunohistochemistry: RNF2 Antibody [NBP1-49966] - Epigenetic remodelers were overexpressed in pancreatitis and pancreatic cancer. Immunohistochemistry staining against Bmi1, Ring1b (RNF2) and H2AK119ub of pancreatic tissue from three week old KPC mice representing morphological normal tissue, from ten week old mice showing preinvasive ductal lesions and from 20 week old KPC mice demonstrating full PDAC (n = 5). High magnification images represent nuclear staining of centroacinar cells (3w-normal) or ADMs (10w-ADM). Bar, 100 um. Image collected and cropped by CiteAb from the following publication (//www.oncotarget.com/fulltext/6717), licensed under a CC-BY license.

Immunohistochemistry: RNF2 Antibody [NBP1-49966] - Epigenetic remodelers were overexpressed in pancreatitis & pancreatic cancer(A) Immunohistochemistry staining of Bmi1, Ring1b & H2AK119ub in eight week old control mice, 48 hours & seven days after the last cerulein injection (n = 5). Representative images include boxes with high magnification to demonstrate nuclear staining of centroacinar (control) & ADM (48h) cells. Bar, 100 μm. (B) Positive stained nuclei of Bmi1, Ring1b & H2AK119ub per high power field (HPF) were counted & quantified. Data are presented as mean ± SEM from three pictures of each animal. (C) Immunohistochemistry staining against Bmi1, Ring1b & H2AK119ub of pancreatic tissue from three week old KPC mice representing morphological normal tissue, from ten week old mice showing preinvasive ductal lesions & from 20 week old KPC mice demonstrating full PDAC (n = 5). High magnification images represent nuclear staining of centroacinar cells (3w-normal) or ADMs (10w-ADM). Bar, 100 μm. (D) Positive stained nuclei of Bmi1, Ring1b & H2AK119ub per high power field (HPF) were counted & quantified. Data are presented as mean ± SEM from three pictures of each animal. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26716510), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

• Reactivity: Hu, Mu, Rt, Pm-Or
• Applications: WB, IHC, IP, ChIP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

RNF2 Antibody - BSA Free Summary

• Immunogen: The immunogen for this product maps to a region between residue 150 and 200 of human Ring Finger Protein 2 using the numbering given in entry NP_009143.1 (GeneID 6045).
• Predicted Species: Rat (100%), Orangutan (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RNF2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation Sequencing
  • Immunohistochemistry 1:500 - 1:2000
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:10000
• Application Notes: Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for RNF2 Antibody - BSA Free

• BAP-1
• BAP1RING finger protein 1B
• DING
• DINGRING finger protein BAP-1
• EC 6.3.2
• HIPI3
• HIPI3RING1BE3 ubiquitin-protein ligase RING2
• Huntingtin-interacting protein 2-interacting protein 3
• Protein DinG
• ring finger protein 2HIP2-interacting protein 3
• RING1B
• RING2
• RING2EC 6.3.2.-
• RNF2

Background

Polycomb group (PcG) of proteins form the multiprotein complexes that are important for the transcription repression of various genes involved in development and cell proliferation. The protein encoded by this gene is one of the PcG proteins. It has been shown to interact with, and suppress the activity of, transcription factor CP2 (TFCP2/CP2). Studies of the mouse counterpart suggested the involvement of this gene in the specification of anterior-posterior axis, as well as in cell proliferation in early development. This protein was also found to interact with huntingtin interacting protein 2 (HIP2), an ubiquitin-conjugating enzyme, and possess ubiquitin ligase activity. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for RNF2 Antibody (NBP1-49966)(2)

Publications using NBP1-49966

• Benitz S, Regel I, Reinhard T et al. Polycomb repressor complex 1 promotes gene silencing through H2AK119 mono-ubiquitination in acinar-to-ductal metaplasia and pancreatic cancer cells. Oncotarget 2016-03-08 [PMID: 26716510]
• Holdt LM, Hoffmann S, Sass K et al. Alu Elements in ANRIL Non-Coding RNA at Chromosome 9p21 Modulate Atherogenic Cell Functions through Trans-Regulation of Gene Networks. PLoS Genet 2013-07-01 [PMID: 23861667] (IP, Human)

Bioinformatics

• Gene Symbol: RNF2
• Entrez:
  • 6045(Human)
  • 19821(Mouse)
• Uniprot:
  • Q99496(Human)