RNF2 Antibody (S04-2B3)

Western Blot: RNF2 Antibody (S04-2B3) [NBP3-14971] - Western blot detection of RNF2 in Hela, A549, HL-60, U2OS, U87-MG cell lysates using RNF2 Rabbit mAb (1:1000 diluted). Observed band size: 38kDa.

Immunohistochemistry: RNF2 Antibody (S04-2B3) [NBP3-14971] - Immunohistochemical of RNF2 in Human lung cancer tissue using RNF2 antibody at dilution 1/50

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clone: S04-2B3
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

RNF2 Antibody (S04-2B3) Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthesized peptide derived from human RNF2 (Uniprot#: Q99496)
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: RNF2
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1/50-1/200
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Western Blot 1/500-1/2000
• Theoretical MW: 38 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA
• Preservative: 0.01% Sodium Azide
• Purity: Affinity purified

Alternate Names for RNF2 Antibody (S04-2B3)

• BAP-1
• BAP1RING finger protein 1B
• DING
• DINGRING finger protein BAP-1
• EC 6.3.2
• HIPI3
• HIPI3RING1BE3 ubiquitin-protein ligase RING2
• Huntingtin-interacting protein 2-interacting protein 3
• Protein DinG
• ring finger protein 2HIP2-interacting protein 3
• RING1B
• RING2
• RING2EC 6.3.2.-
• RNF2

Background

Polycomb group (PcG) of proteins form the multiprotein complexes that are important for the transcription repression of various genes involved in development and cell proliferation. The protein encoded by this gene is one of the PcG proteins. It has been shown to interact with, and suppress the activity of, transcription factor CP2 (TFCP2/CP2). Studies of the mouse counterpart suggested the involvement of this gene in the specification of anterior-posterior axis, as well as in cell proliferation in early development. This protein was also found to interact with huntingtin interacting protein 2 (HIP2), an ubiquitin-conjugating enzyme, and possess ubiquitin ligase activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RNF2