RIPK2 Knockout HeLa Cell Lysate

Product Discontinued

RIPK2 Knockout HeLa Cell Lysate Summary

• Preparation Method: Knockout achieved by using CRISPR/Cas9,2 bp deletion in exon 1 and 1 bp insertion in exon 1
• Gene: RIPK2

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: You will receive 1 vial (100ug) of knockout cell lysate and 1 vial (100ug) of Parental cell lysate. Lysate can be diluted with 1X SDS sample buffer and will be stable at -20 degrees C for 12 months. Minimize freeze-thaw cycles.

Packaging, Storage & Formulations

• Storage: Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
• Buffer: 0.1 mg cell homogenate lyophilized in RIPA buffer made with double-knockout cell lines.
• Concentration: LYOPH
• Reconstitution Instructions: To use as WB negative control, spin down briefly and resuspend in 100 uL 1xSDS sample buffer (2% SDS, 60 mM Tris-HCl pH 6.8, 10% Glycerol, 0.02% Bromophenol blue, 60 mM beta-mercaptoethanol). Boil the lysate for 3 - 5 minutes before loading it onto gel.

Lysate Details for Array

• Type: Knockout HeLa Cell

Notes

Powered by EDIGENE. Validation of antibody specificity is critical and verification of antibody performance against knockout samples is one way to guarantee that an antibody recognizes a specific target. Novus' KO cell lysate can be used as a negative control for western blots and to confirm the specificity of antibodies.

Alternate Names for RIPK2 Knockout HeLa Cell Lysate

• CARD3
• CARD-containing IL-1 beta ICE-kinase
• CARD-containing interleukin-1 beta-converting enzyme (ICE)-associated kinase
• CARD-containing interleukin-1 beta-converting enzyme-associated kinase
• CARDIAKCCK
• EC 2.7.11
• EC 2.7.11.1
• GIG30
• growth-inhibiting gene 30
• receptor interacting protein 2
• receptor-interacting protein (RIP)-like interacting caspase-like apoptosisregulatory protein (CLARP) kinase
• Receptor-interacting protein 2
• receptor-interacting serine/threonine-protein kinase 2
• receptor-interacting serine-threonine kinase 2
• RICK
• RICKGIG30
• RIP2
• RIP-2
• RIP2CARD-carrying kinase
• RIPK2
• RIP-like-interacting CLARP kinase

Background

This gene encodes a member of the receptor-interacting protein (RIP) family of serine/threonine protein kinases. The encoded protein contains a C-terminal caspase activation and recruitment domain (CARD), and is a component of signaling complexes in both the innate and adaptive immune pathways. It is a potent activator of NF-kappaB and inducer of apoptosis in response to various stimuli. [provided by RefSeq, Jul 2008]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: RIPK2