RIPK1/RIP1 Antibody Blocking Peptide

• Reactivity: Hu
• Applications: AC
• Concentration: 0.2 mg/ml

RIPK1/RIP1 Antibody Blocking Peptide Summary

• Description: A blocking peptide containing 15 amino acids near the amino terminus of human RIPK1.

  Source: Synthetic

  (Accession #: NP_003795)

• Source: Synthetic
• Protein/Peptide Type: Antibody Blocking Peptide
• Gene: RIPK1
• Purity: N/A

Applications/Dilutions

• Dilutions:
  • Antibody Competition
• Application Notes: This peptide is useful as a blocking peptide for NBP1-77077.
  
  For further blocking peptide related protocol, click here.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS pH 7.2 (10 mM NaH2PO4, 10 mM Na2HPO4, 130 mM NaCl) containing 0.1% bovine serum albumin.
• Preservative: 0.02% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: N/A

Alternate Names for RIPK1/RIP1 Antibody Blocking Peptide

• Cell death protein RIP
• EC 2.7.11.1
• receptor (TNFRSF)-interacting serine-threonine kinase 1
• receptor interacting protein
• Receptor-interacting protein 1
• receptor-interacting serine/threonine-protein kinase 1
• RIP
• Rip1 kinase
• RIP1
• RIP-1
• RIPFLJ39204
• RIPK1
• Serine/threonine-protein kinase RIP

Background

RIP (Receptor Interacting Protein) is a 74 kD Ser/Thr kinase which interacts with CD95 (Fas/APO1) receptor and the tumor necrosis factor receptor (TNFR1). It is a cell death domain adapter protein which can bind to the adapter proteins TRADD, RAID (CRADD) and TRAF2. RIP contains an N terminal region with homology to protein kinases, an intermediate domain capable of association with MAPKKK and a C terminal region containing an intracellular death domain motif. RIP activates both p38 MAP Kinase and SAPK families. In vitro, RIP induces apoptosis, as well as SAPK/JNK and NF-kB activation. NF-kB activation through TRADD, TRAF2 and RIP can be triggered also by DR3/APO3 upon activation with APO3/Tweak ligand. DR4 and DR5 also use FADD, TRADD, and RIP in their signal transduction pathways. RIP deficient mice fail to thrive, displaying extensive apoptosis in both lymphoid and adipose tissues and dying at 1-3 days of age. RIP possesses kinase activity as it autophosphorylates itself on serine and threonine residues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Publications for RIPK1/RIP1 Peptide (NBP1-77077PEP)(1)

Publication using NBP1-77077PEP

• Al-Lamki RS, Lu W, Manalo P et al. Tubular epithelial cells in renal clear cell carcinoma express high RIPK1/3 and show increased susceptibility to TNF receptor 1-induced necroptosis. Cell Death Dis 2016-06-30 [PMID: 27362805]

Bioinformatics

• Gene Symbol: RIPK1