RIPK1/RIP1 Antibody (7H10)

Western Blot: RIPK1/RIP1 Antibody (7H10) [NBP2-11523] - Analysis of anti-RIPK1 /RIP antibody with Lane 1: K562 cell lysate; Lane 2: HeLa cell lysate; Lane 3: SK-N-MC cell lysate.

Immunohistochemistry-Paraffin: RIPK1/RIP1 Antibody (7H10) [NBP2-11523] - Analysis of anti-RIPK1 /RIP antibody with human small intestine at dilution 1:200.

Product Discontinued

RIPK1/RIP1 Antibody (7H10) Summary

• Immunogen: Recombinant human protein purified from E. coli (RIPK1/RIP1).
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: RIPK1
• Purity: Ammonium sulfate precipitation

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:200
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:500-1:2000

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: HEPES, 0.01% BSA, 0.15M NaCl and 50% Glycerol
• Preservative: 0.03% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Ammonium sulfate precipitation

Alternate Names for RIPK1/RIP1 Antibody (7H10)

• Cell death protein RIP
• EC 2.7.11.1
• receptor (TNFRSF)-interacting serine-threonine kinase 1
• receptor interacting protein
• Receptor-interacting protein 1
• receptor-interacting serine/threonine-protein kinase 1
• RIP
• Rip1 kinase
• RIP1
• RIP-1
• RIPFLJ39204
• RIPK1
• Serine/threonine-protein kinase RIP

Background

RIP (Receptor Interacting Protein) is a 74 kD Ser/Thr kinase which interacts with CD95 (Fas/APO1) receptor and the tumor necrosis factor receptor (TNFR1). It is a cell death domain adapter protein which can bind to the adapter proteins TRADD, RAID (CRADD) and TRAF2. RIP contains an N terminal region with homology to protein kinases, an intermediate domain capable of association with MAPKKK and a C terminal region containing an intracellular death domain motif. RIP activates both p38 MAP Kinase and SAPK families. In vitro, RIP induces apoptosis, as well as SAPK/JNK and NF-kB activation. NF-kB activation through TRADD, TRAF2 and RIP can be triggered also by DR3/APO3 upon activation with APO3/Tweak ligand. DR4 and DR5 also use FADD, TRADD, and RIP in their signal transduction pathways. RIP deficient mice fail to thrive, displaying extensive apoptosis in both lymphoid and adipose tissues and dying at 1-3 days of age. RIP possesses kinase activity as it autophosphorylates itself on serine and threonine residues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RIPK1
• Entrez:
  • 8737(Human)
• Uniprot:
  • Q13546()