Ribosomal Protein S6/RPS6 [p Ser235] Antibody (MC27)

Western Blot: RPS6 [p Ser235] Antibody (MC27) [NBP2-29667] - 3T3 cell lysate from serum starved cells (lane 1), and serum-stimulated cells (lane 2) were resolved by SDS-PAGE, transferred to nitrocellulose, and probed with anti-phospho-Ribosomal Protein S6, MC27 (1:1,000 dilution). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates phosphorylated ribosomal protein S6 (30 kDa).

Product Discontinued

Ribosomal Protein S6/RPS6 [p Ser235] Antibody (MC27) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide containing the sequence RRL[pS]SL in which pS corresponds to phospho-serine at residue 235 of human ribosomal protein S6. The immunizing sequence is identical in mouse, rat, chicken and Xenopus.
• Modification: p Ser235
• Specificity: Recognizes ribosomal protein S6 only when phosphorylated on Ser235.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: RPS6
• Purity: Tissue culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500-1:2000
• Application Notes: Western Blot Analysis: A given dilution of this lot detected phosphorylated ribosomal protein S6 in lysates from serum stimulated 3T3 cells.
• Theoretical MW: 30 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human, Mouse, Rat

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: Tissue culture supernatant
• Preservative: 0.05% Sodium Azide
• Purity: Tissue culture supernatant

Notes

Relevant Entrez Gene Numbers: NM_001010.2

Alternate Names for Ribosomal Protein S6/RPS6 [p Ser235] Antibody (MC27)

• Phosphoprotein NP33, 40S ribosomal protein S6
• pS6
• Ribosomal Protein S6
• RPS6
• S6

Background

S6 (Ribosomal protein S6, RpS6, 40S ribosomal protein S6) is known to play a role in cell growth and proliferation. It lays downstream of both the PI3K/Akt/mTOR pathway as a substrate of p70 S6 kinase and the Ras/Erk pathway as a substrate of RSK. S6 is phosphorylated in response to stimulation by growth factors, tumor promoting agents, and mitogens. It is dephosphorylated at growth arrest. There are two major areas of S6 phosphorylation; Ser235/Ser236 that is known to be phosphorylated by both p70S6 Kinase as well as RSK as well as Ser240/244 which is thought to only be phosphorylated by p70S6K.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RPS6
• Uniprot:
  • P62753()