Ribosomal protein L26 Antibody

Western Blot: Ribosomal protein L26 Antibody [NB100-2131] - Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug) and mouse NIH3T3 (M; 50 ug) cells. Affinity purified rabbit anti-RPL26 antibody (NB100-2131) used for WB at 0.04 ug/ml (A) and 1 ug/ml (B), and used for IP at 3 ug/mg lysate.

Immunohistochemistry-Paraffin: Ribosomal protein L26 Antibody [NB100-2131] - Sample: FFPE section of human skin carcinoma. Antibody: Affinity purified rabbit anti-expndtw-4 RPL26 used at a dilution of 1:200 (1ug/ml). Detection: DAB

• Reactivity: Hu, Mu, Rt, Bv, Ch
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

Ribosomal protein L26 Antibody Summary

• Description: Novus Biologicals Rabbit Ribosomal protein L26 Antibody (NB100-2131) is a polyclonal antibody validated for use in IHC, WB and IP. Anti-Ribosomal protein L26 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 100 and the C-terminus (residue 145) of human Ribosomal Protein L26 using the numbering given in entry NP_000978.1 (GeneID 6154).
• Predicted Species: Rat (100%), Chicken (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RPL26
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:200 - 1:1000
  • Immunohistochemistry-Paraffin 1:250-1:500
  • Immunoprecipitation 1-4 ug/mg of lysate
  • Western Blot 1:2500-1:10000
• Application Notes: Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
• Control:

  Ribosomal protein L26 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Ribosomal protein L26 Antibody

• ribosomal protein L26,60S ribosomal protein L26

Background

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L24P family of ribosomal proteins. It is located in the cytoplasm. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Ribosomal protein L26 Antibody (NB100-2131)(1)

Publication using NB100-2131

• Geissler R, Golbik RP, Behrens SE et al. The DEAD-box helicase DDX3 supports the assembly of functional 80S ribosomes. Nucleic Acids Res 2012-06-01 [PMID: 22323517]

Bioinformatics

• Gene Symbol: RPL26
• Entrez:
  • 6154(Human)
  • 19941(Mouse)
• Uniprot:
  • NP_000978.1(Human)