Ribophorin II Antibody

Images

 
Western Blot: Ribophorin II Antibody [NBP1-62480] - Titration: 0.25ug/ml Positive Control: HepG2 cell lysate.
Immunocytochemistry/ Immunofluorescence: Ribophorin II Antibody [NBP1-62480] - Formalin Fixed Paraffin Embedded Tissue: Human Bronchial Epithelial Tissue Observed Staining: Cytoplasmic
Immunohistochemistry-Paraffin: Ribophorin II Antibody [NBP1-62480] - Human Skin Tissue Squamous epithelial cells (Indicated with Arrows), 4-8ug/ml.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Ribophorin II Antibody Summary

Immunogen
Synthetic peptides corresponding to RPN2(ribophorin II) The peptide sequence was selected from the N terminal of RPN2. Peptide sequence ASQEALSALTARLSKEETVLATVQALQTASHLSQQADLRSIVEEIEDLVA.
Specificity
This product is specific to Subunit or Isoform: 2.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RPN2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Theoretical MW
67 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS and 2% Sucrose
Preservative
0.09% Sodium Azide
Purity
Immunogen affinity purified

Notes

The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.

Alternate Names for Ribophorin II Antibody

  • RIBIIREC 2.4.1.119
  • ribophorin IIDolichyl-diphosphooligosaccharide--protein glycosyltransferase 63 kDa subunit
  • ribophorin-2
  • RPNII
  • RPN-IIdolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 2
  • SWP1

Background

RPN2 is a type I integral membrane protein found only in the rough endoplasmic reticulum. The protein is part of an N-oligosaccharyl transferase complex that links high mannose oligosaccharides to asparagine residues found in the Asn-X-Ser/Thr consensus motif of nascent polypeptide chains.This gene encodes a type I integral membrane protein found only in the rough endoplasmic reticulum. The encoded protein is part of an N-oligosaccharyl transferase complex that links high mannose oligosaccharides to asparagine residues found in the Asn-X-Ser/Thr consensus motif of nascent polypeptide chains. This protein is similar in sequence to the yeast oligosaccharyl transferase subunit SWP1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol RPN2
Uniprot