| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-25160SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59690-25ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-47602-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-25159SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-09001-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-81059-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-55553-25ul | |||
![Immunohistochemistry: Rhodopsin Antibody (B630) [NBP2-25160] - Rhodopsin expression and the prevalence of rod photoreceptors in the canine retina. (A) Fluorescence micrographs showing rhodopsin expression (red) in the ABCA4+/+ (left), ABCA4+/- (middle), and ABCA4-/- (right) rod outer segments. Scale bar = 10 um. Image collected and cropped by CiteAb from the following publication (doi.org/10.1371/journal.pgen.1007873) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Rhodopsin-Antibody-B630-Immunohistochemistry-NBP2-25160-img0004.jpg "Immunohistochemistry: Rhodopsin Antibody (B630) [NBP2-25160] - Rhodopsin expression and the prevalence of rod photoreceptors in the canine retina. (A) Fluorescence micrographs showing rhodopsin expression (red) in the ABCA4+/+ (left), ABCA4+/- (middle), and ABCA4-/- (right) rod outer segments. Scale bar = 10 um. Image collected and cropped by CiteAb from the following publication (doi.org/10.1371/journal.pgen.1007873) licensed under a CC-BY license.")
![Western Blot: Rhodopsin Antibody (B630) [NBP2-25160] - Western blot analyses of ABCA4 (above), GAPDH (middle), and RHO (below) protein levels in retinal tissue of dogs with the three different genotypes. Image collected and cropped by CiteAb from the following publication. 50 ug of protein samples were resolved by SDS-PAGE, transferred to nitrocellulose membrane, and immunoblotted with the following primary antibodies: ABCA4 (NBP1-30032, 1:1000) and Rhodopsin (NBP2-25160, 1:5000), followed by Anti-Mouse IgG horseradish peroxidase-conjugated secondary antibody (R&D Systems, HAF007, 1:5000. Image collected and cropped by CiteAb from the following publication (doi.org/10.1371/journal.pgen.1007873) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Rhodopsin-Antibody-B630-Western-Blot-NBP2-25160-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Rhodopsin Antibody (B630) [NBP2-25160] - High magnification confocal image of pig retinal section stained with NBP2-25160 (Green). Rhodopsin is most abundant in the rod outer segments (ROS) of retina, clearly localized in rod membranes. The rod inner segments (RIS) and rod nuclei in the outer nuclear layer (ONL) are also seen in this image. Nuclear DNA was stained with DAPI (blue).](http://images.novusbio.com/fullsize/Rhodopsin-Antibody-B630-Immunocytochemistry-Immunofluorescence-NBP2-25160-img0002.jpg)
![Western Blot: Rhodopsin Antibody (B630) [NBP2-25160] - Blot of bovine retinal extracts. The antibody stains a band corresponing to retinal Rhodopsin at about 35kDa. Bands about 70 kDa and 140 kDa are aggregated forms of Rhodopsin. Note, due to the highly hydrophobic nature of Rhodopsin, it is important to avoid boiling samples containing this protein in SDS-PAGE sample buffer, as this will result in even more extensive aggregation of the Rhodopsin protein and appearance of more of this high molecular weight material.](http://images.novusbio.com/fullsize/Rhodopsin-Antibody-B630-Western-Blot-NBP2-25160-img0003.jpg)
![Western Blot: Rhodopsin Antibody (4D2) [NBP2-59690] - Western Blot analysis of Human A549 cells showing detection of ~38.9kDa Rhodopsin protein using Mouse Anti-Rhodopsin Monoclonal Antibody, Clone 4D2 (NBP2-59690). Lane 1: MW ladder. Lane 2: Human A549 Cells 15 ug). Load: 15 ug. Block: 5% Skim Milk Powder in TBST. Primary Antibody: Mouse Anti-Rhodopsin Monoclonal Antibody (NBP2-59690) at 1:1000 for 2.5 hours at RT with shaking . Secondary Antibody: Goat anti-mouse IgG:HRP at 1:1000 for 1 hour at RT with shaking . Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. Predicted/Observed Size: ~38.9kDa. Other Band(s): Band appears at ~75 kDa indicating detection of the Rhodopsin dimer.](http://images.novusbio.com/fullsize/Rhodopsin-Antibody-4D2-Western-Blot-NBP2-59690-img0005.jpg "Western Blot: Rhodopsin Antibody (4D2) [NBP2-59690] - Western Blot analysis of Human A549 cells showing detection of ~38.9kDa Rhodopsin protein using Mouse Anti-Rhodopsin Monoclonal Antibody, Clone 4D2 (NBP2-59690). Lane 1: MW ladder. Lane 2: Human A549 Cells 15 ug). Load: 15 ug. Block: 5% Skim Milk Powder in TBST. Primary Antibody: Mouse Anti-Rhodopsin Monoclonal Antibody (NBP2-59690) at 1:1000 for 2.5 hours at RT with shaking . Secondary Antibody: Goat anti-mouse IgG:HRP at 1:1000 for 1 hour at RT with shaking . Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. Predicted/Observed Size: ~38.9kDa. Other Band(s): Band appears at ~75 kDa indicating detection of the Rhodopsin dimer.")
![Immunohistochemistry: Rhodopsin Antibody (4D2) [NBP2-59690] - Immunohistochemistry analysis using Mouse Anti-Rhodopsin Monoclonal Antibody, Clone 4D2 (NBP2-59690). Tissue: retina. Species: Mouse. Primary Antibody: Mouse Anti-Rhodopsin Monoclonal Antibody (NBP2-59690) at 1:1000. Secondary Antibody: FITC Goat Anti-Mouse (green). Counterstain: DAPI (blue) nuclear stain. Localization: Staining of photoreceptor outer segment (OS). Other layers of the retina: IS inner segment; ONL outer nuclear layer; OPL outer plexiform layer; INL inner nuclear layer; IPL inner plexiform layer; GCL ganglion cell layer..](http://images.novusbio.com/fullsize/Rhodopsin-Antibody-4D2-Immunohistochemistry-NBP2-59690-img0006.jpg)
![Western Blot: Rhodopsin Antibody (4D2) [NBP2-59690] - Western Blot analysis of Bovine photoreceptor membranes showing detection of Rhodopsin protein using Mouse Anti-Rhodopsin Monoclonal Antibody, Clone 4D2 (NBP2-59690). Lane 1: MW ladder. Lane 2: 10 ug. Lane 3: 5 ug. Lane 4: 2.5 ug.. Primary Antibody: Mouse Anti-Rhodopsin Monoclonal Antibody (NBP2-59690) at 1:1000.](http://images.novusbio.com/fullsize/Rhodopsin-Antibody-4D2-Western-Blot-NBP2-59690-img0004.jpg)
![Immunohistochemistry-Frozen: Rhodopsin Antibody (4D2) [NBP2-59690] - Zebrafish retina (outer nuclear layer). PFA and methanol fixed 7 um sections. Rhodopsin antibody O/N incubation at 1:500. IHC-Fr image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Rhodopsin-Antibody-4D2-Immunohistochemistry-Frozen-NBP2-59690-img0002.jpg)
![Western Blot: Rhodopsin Antibody (1D4) [NBP1-47602] - Quantification of rhodopsin protein (western blot) levels in the neural retina of WT (n = 7) and hFATP1TG (n = 8) mice. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28672005/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Rhodopsin-Antibody-1D4-Western-Blot-NBP1-47602-img0007.jpg "Western Blot: Rhodopsin Antibody (1D4) [NBP1-47602] - Quantification of rhodopsin protein (western blot) levels in the neural retina of WT (n = 7) and hFATP1TG (n = 8) mice. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28672005/) licensed under a CC-BY license.")
![Immunohistochemistry: Rhodopsin Antibody (1D4) [NBP1-47602] - Immunohistochemistry analysis using Mouse Anti-Rhodopsin Monoclonal Antibody, Clone 1D4 (NBP1-47602). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Rhodopsin Monoclonal Antibody (NBP1-47602) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Dull epidermal staining.](http://images.novusbio.com/fullsize/Rhodopsin-Antibody-1D4-Immunohistochemistry-NBP1-47602-img0006.jpg)
![Western Blot: Rhodopsin Antibody (1D4) [NBP1-47602] - Western Blot analysis of Human Cell lysates showing detection of Rhodopsin protein using Mouse Anti-Rhodopsin Monoclonal Antibody, Clone 1D4 (NBP1-47602). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Rhodopsin Monoclonal Antibody (NBP1-47602) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.](http://images.novusbio.com/fullsize/Rhodopsin-Antibody-1D4-Western-Blot-NBP1-47602-img0005.jpg)
![Western Blot: Rhodopsin Antibody (A531) [NBP2-25159] - Blot of bovine retinal extracts. The antibody stains a band corresponing to retinal Rhodopsin at about 35kDa. Bands about 70 kDa and 140 kDa are aggregated forms of Rhodopsin. Note, due to the highly hydrophobic nature of Rhodopsin, it is important not to boil a sample containing it in SDS-PAGE sample buffer, as this will result in more extensive aggregation of the Rhodopsin protein.](http://images.novusbio.com/fullsize/Rhodopsin-Antibody-A531-Western-Blot-NBP2-25159-img0004.jpg "Western Blot: Rhodopsin Antibody (A531) [NBP2-25159] - Blot of bovine retinal extracts. The antibody stains a band corresponing to retinal Rhodopsin at about 35kDa. Bands about 70 kDa and 140 kDa are aggregated forms of Rhodopsin. Note, due to the highly hydrophobic nature of Rhodopsin, it is important not to boil a sample containing it in SDS-PAGE sample buffer, as this will result in more extensive aggregation of the Rhodopsin protein.")
![Immunocytochemistry/Immunofluorescence: Rhodopsin Antibody (A531) [NBP2-25159] - Pig retinal section stained with NBP2-25159 (green) and counterstained with rabbit polyclonal antibody to NF-M NB300-133 (red) and DNA (blue). Rhodopsin is most abundant in the outer segments of retina (OS), NF-M is abundant in the optic nerve fiber layer (ONFL), but seen in processes and neurons in other regions also. Other layers are pigmented epithelium (PE), outer and inner nuclear layers (ONL, INL), outer and inner plexiform layers (OPL, IPL) and ganglion cell layer (GCL).](http://images.novusbio.com/fullsize/Rhodopsin-Antibody-A531-Immunocytochemistry-Immunofluorescence-NBP2-25159-img0003.jpg)
![Boiled and unboiled various tissue extracts (50 �g) were separated by 10% SDS-PAGE, and the membrane was blotted with Rhodopsin antibody [HL2668] (NBP3-48717) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-48717_rabbit-rhodopsin-mab-hl2668-western-blot-1411202518373813.jpg "<b>Orthogonal Strategies Validation. </b>Boiled and unboiled various tissue extracts (50 �g) were separated by 10% SDS-PAGE, and the membrane was blotted with Rhodopsin antibody [HL2668] (NBP3-48717) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![<b>Orthogonal Strategies Validation. </b>Rhodopsin antibody [HL2668] (NBP3-48717) detects Rhodopsin protein by flow cytometry analysis.Sample: Non-transfected and transfected 293T cells were fixed in 4% paraformaldehyde at 4�C for 15 min and washed with 0.1% saponin buffer. The cells were following stained with Rhodopsin antibody [HL2668] (NBP3-48717) diluted at 1:50 or a Rabbit monoclonal IgG isotype control at 4�C for 1 hour.](http://images.novusbio.com/fullsize/nbp3-48717_rabbit-rhodopsin-mab-hl2668-flow-cytometry-1411202518365924.jpg)
![Human tissue extract was separated by 10% SDS-PAGE, and the membrane was blotted with Rhodopsin antibody [HL2668] (NBP3-48717) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-48717_rabbit-rhodopsin-mab-hl2668-western-blot-1411202518324111.jpg)
![<b>Orthogonal Strategies Validation. </b>Unboiled non-transfected (�) and transfected (+) 293T whole cell extracts (30 �g) were separated by 10% SDS-PAGE, and the membrane was blotted with Rhodopsin antibody [HL2668] (NBP3-48717) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-48717_rabbit-rhodopsin-mab-hl2668-western-blot-1411202518424110.jpg)
![Rat tissue extract (30 �g) was separated by 10% SDS-PAGE, and the membrane was blotted with Rhodopsin antibody [HL2668] (NBP3-48717) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-48717_rabbit-rhodopsin-mab-hl2668-western-blot-14112025184336.jpg)
![Rhodopsin antibody [HL2668] detects Rhodopsin protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse eye.Rhodopsin stained by Rhodopsin antibody [HL2668] (NBP3-48717) diluted at 1:100.Red: beta Tubulin 3/ Tuj1 stained by beta Tubulin 3/ Tuj1 antibody [GT11710] diluted at 1:500.Blue: Fluoroshield with DAPI .Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-48717_rabbit-rhodopsin-mab-hl2668-immunohistochemistry-paraffin-1411202518401615.jpg)
![Rhodopsin antibody [HL2668] detects Rhodopsin protein by immunohistochemical analysis.Sample: Paraffin-embedded rat eye.Rhodopsin stained by Rhodopsin antibody [HL2668] (NBP3-48717) diluted at 1:100.Red: beta Tubulin 3/ Tuj1 stained by beta Tubulin 3/ Tuj1 antibody [GT11710] diluted at 1:500.Blue: Fluoroshield with DAPI .Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-48717_rabbit-rhodopsin-mab-hl2668-immunohistochemistry-paraffin-1411202518415219.jpg)
![<b>Orthogonal Strategies Validation. </b>Non-transfected (�) and transfected (+) boiled and unboiled 293T whole cell extracts (30 �g) were separated by 10% SDS-PAGE, and the membrane was blotted with Rhodopsin antibody [HL2668] (NBP3-48717) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-48717_rabbit-rhodopsin-mab-hl2668-western-blot-141120251840548.jpg)
![Western Blot: Rhodopsin Antibody (RET-P1) [NB120-3267] - Analysis of IMR-5 Cells.](http://images.novusbio.com/fullsize/Rhodopsin-Antibody-RET-P1-Western-Blot-NB120-3267-img0004.jpg "Western Blot: Rhodopsin Antibody (RET-P1) [NB120-3267] - Analysis of IMR-5 Cells.")
![Immunohistochemistry-Paraffin: Rhodopsin Antibody (RET-P1) [NB120-3267] - Human brain stained with Rhodopsin antibody using peroxidase-conjugate and AEC chromogen. Note cytoplasmic staining of neurons.](http://images.novusbio.com/fullsize/Rhodopsin-Antibody-RET-P1-Immunohistochemistry-Paraffin-NB120-3267-img0005.jpg)
![Immunohistochemistry-Paraffin: Rhodopsin Antibody [NLS1052] - Eye, Retina](http://images.novusbio.com/fullsize/Rhodopsin-Antibody-Immunohistochemistry-Paraffin-NLS1052-img0004.jpg "Immunohistochemistry-Paraffin: Rhodopsin Antibody [NLS1052] - Eye, Retina")
![Immunohistochemistry-Paraffin: Rhodopsin Antibody [NLS1052] - Analysis of anti-RHO / Rhodopsin antibody with human eye, retina.](http://images.novusbio.com/fullsize/Rhodopsin-Antibody-Immunohistochemistry-Paraffin-NLS1052-img0002.jpg)
![Immunohistochemistry-Paraffin: Rhodopsin Antibody [NLS1052] - Eye, Retina](http://images.novusbio.com/fullsize/Rhodopsin-Antibody-Immunohistochemistry-Paraffin-NLS1052-img0003.jpg)
![ELISA: Rhodopsin Antibody (Rho 1D4) - Chimeric [NBP3-09001] - Using 1D4 (Ab00337) and Multi Tag protein 2. Binding curves of the mouse IgG1 chimeric version of the Rhodopsin antibody 1D4 (Ab00337-1.1; dark blue line) and isotype control (Ab00102-1.1, anti-fluorescein; orange line) to an ELISA plate coated with Multi Tag protein 2 (Pr00565, Absolute Antibody) at a concentration of 5 ug/ml. Ab02007 antibody was titrated on a 3-fold serial dilution starting at 10,000 ng/mL. Anti-mouse IgG was used as secondary antibody for detection at a dilution of 1:4,000.](http://images.novusbio.com/fullsize/Rhodopsin-Antibody-Rho-1D4-Chimeric-ELISA-NBP3-09001-img0001.jpg "ELISA: Rhodopsin Antibody (Rho 1D4) - Chimeric [NBP3-09001] - Using 1D4 (Ab00337) and Multi Tag protein 2. Binding curves of the mouse IgG1 chimeric version of the Rhodopsin antibody 1D4 (Ab00337-1.1; dark blue line) and isotype control (Ab00102-1.1, anti-fluorescein; orange line) to an ELISA plate coated with Multi Tag protein 2 (Pr00565, Absolute Antibody) at a concentration of 5 ug/ml. Ab02007 antibody was titrated on a 3-fold serial dilution starting at 10,000 ng/mL. Anti-mouse IgG was used as secondary antibody for detection at a dilution of 1:4,000.")
![Western Blot: Rhodopsin Antibody (Rho 1D4) - Azide and BSA Free [NBP2-81059] - Rhodopsin expressing plasmid transfected (A) and non-transfected (B) HEK293 cells lysate (3ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with NBP2-81059 at 0.1ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.](http://images.novusbio.com/fullsize/nbp2-81059_rabbit-monoclonal-rhodopsin-antibody-rho-1d4-74202522141212.png "Western Blot: Rhodopsin Antibody (Rho 1D4) - Azide and BSA Free [NBP2-81059] - Rhodopsin expressing plasmid transfected (A) and non-transfected (B) HEK293 cells lysate (3ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with NBP2-81059 at 0.1ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.")
![Western Blot: Rhodopsin Antibody (5N3G9) [NBP3-16655] - Western blot analysis of extracts of various cell lines, using Rhodopsin Rabbit mAb (NBP3-16655) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/Rhodopsin-Antibody-5N3G9-Western-Blot-NBP3-16655-img0002.jpg "Western Blot: Rhodopsin Antibody (5N3G9) [NBP3-16655] - Western blot analysis of extracts of various cell lines, using Rhodopsin Rabbit mAb (NBP3-16655) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Immunohistochemistry-Paraffin: Rhodopsin Antibody (5N3G9) [NBP3-16655] - Immunohistochemistry of paraffin-embedded mouse retina using Rhodopsin Rabbit mAb (NBP3-16655) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Rhodopsin-Antibody-5N3G9-Immunohistochemistry-Paraffin-NBP3-16655-img0001.jpg)
![Immunohistochemistry-Paraffin: Rhodopsin Antibody [NBP2-55553] - Staining of human retina shows strong cytoplasmic positivity in rod segments.](http://images.novusbio.com/fullsize/Rhodopsin-Antibody-Immunohistochemistry-Paraffin-NBP2-55553-img0003.jpg "Immunohistochemistry-Paraffin: Rhodopsin Antibody [NBP2-55553] - Staining of human retina shows strong cytoplasmic positivity in rod segments.")
![Western Blot: Rhodopsin Overexpression Lysate (Adult Normal) [NBP2-04162] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Rhodopsin.](http://images.novusbio.com/fullsize/Rhodopsin-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-04162-img0001.jpg "Western Blot: Rhodopsin Overexpression Lysate (Adult Normal) [NBP2-04162] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Rhodopsin.")