RFPL1 Antibody (OTI1D3)

Western Blot: RFPL1 Antibody (1D3) [NBP2-02835] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RFPL1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RFPL1.

Immunocytochemistry/Immunofluorescence: RFPL1 Antibody (1D3) [NBP2-02835] Staining of COS7 cells transiently transfected by pCMV6-ENTRY RFPL1.

Flow Cytometry: RFPL1 Antibody (1D3) [NBP2-02835] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-RFPL1 antibody, and then analyzed by flow cytometry.

• Reactivity: Hu
• Applications: WB, Flow, ICC/IF
• Clone: OTI1D3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.72 mg/ml

RFPL1 Antibody (OTI1D3) Summary

• Immunogen: Full length human recombinant protein of human RFPL1 (NP_066306) produced in HEK293T cell.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: RFPL1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Western Blot 1:2000
• Theoretical MW: 35.3 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 0.72 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for RFPL1 Antibody (OTI1D3)

• MGC132428
• ret finger protein-like 1
• RFPL1L
• RNF78RING finger protein 78

Background

RFPL1 is involved in ubiquitination and zinc ion binding.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RFPL1
• Entrez:
  • 5988(Human)