RGS1 Antibody

Images

 
Western Blot: RGS1 Antibody [NB100-1029] - (1ug/ml) of HepG2 lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Immunohistochemistry-Paraffin: RGS1 Antibody [NB100-1029] - staining of paraffin embedded Human Thymus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunohistochemistry-Paraffin: RGS1 Antibody [NB100-1029] - staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

RGS1 Antibody Summary

Immunogen
Peptide with sequence C-NLLNDLQANSLK corresponding to C-Terminus according to NP_002913.3.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
RGS1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5 - 2 ug/ml
  • Immunohistochemistry 2.5 ug/ml
  • Immunohistochemistry-Paraffin 2.5 ug/ml
  • Peptide ELISA Detection limit 1:32000
Application Notes
WB: Approx. 25 kDa band observed in HepG2 lysate.
IHC: Paraffin embedded Human Thymus and Small Intestine.
Publications
Read Publication using NB100-1029.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat, Canine, Bovine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for RGS1 Antibody

  • 1R20Early response protein 1R20
  • BL34B-cell activation protein BL34
  • IER1
  • IR20immediate-early response 1, B-cell specific
  • regulator of G-protein signaling 1
  • regulator of G-protein signalling 1

Background

This gene encodes a member of the regulator of G protein signalling family. This protein is located on the cytosolic side of the plasma membrane and contains a conserved, 120 amino acid motif called the RGS domain. The protein attenuates the signalling activity of G proteins by binding to activated, GTP bound G alpha subunits and acting as a GTPase activating protein (GAP), increasing the rate of conversion of the GTP to GDP. This hydrolysis allows the G alpha subunits to bind G beta/gamma subunit heterodimers, forming inactive G protein heterotrimers, thereby terminating the signal.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for RGS1 Antibody (NB100-1029)(1)

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Video Protocols

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol RGS1
Entrez
Uniprot