RGS1 Antibody

Western Blot: RGS1 Antibody [NB100-1029] - (1ug/ml) of HepG2 lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Immunohistochemistry-Paraffin: RGS1 Antibody [NB100-1029] - staining of paraffin embedded Human Thymus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Immunohistochemistry-Paraffin: RGS1 Antibody [NB100-1029] - staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu, Mu, Rt, Bv, Ca
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

RGS1 Antibody Summary

• Immunogen: Peptide with sequence C-NLLNDLQANSLK corresponding to C-Terminus according to NP_002913.3.
• Predicted Species: Mouse (100%), Rat (100%), Canine (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: RGS1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 2.5 ug/ml
  • Immunohistochemistry-Paraffin 2.5 ug/ml
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.5 - 2 ug/ml
• Application Notes: WB: Approx. 25 kDa band observed in HepG2 lysate.
  IHC: Paraffin embedded Human Thymus and Small Intestine.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat, Canine, Bovine.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for RGS1 Antibody

• 1R20
• 1R20Early response protein 1R20
• B-cell activation protein BL34
• BL34
• Early response protein 1R20
• HEL-S-87
• IER1
• IR20
• IR20immediate-early response 1, B-cell specific
• regulator of G-protein signaling 1
• regulator of G-protein signalling 1
• RGS1

Background

This gene encodes a member of the regulator of G protein signalling family. This protein is located on the cytosolic side of the plasma membrane and contains a conserved, 120 amino acid motif called the RGS domain. The protein attenuates the signalling activity of G proteins by binding to activated, GTP bound G alpha subunits and acting as a GTPase activating protein (GAP), increasing the rate of conversion of the GTP to GDP. This hydrolysis allows the G alpha subunits to bind G beta/gamma subunit heterodimers, forming inactive G protein heterotrimers, thereby terminating the signal.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for RGS1 Antibody (NB100-1029)(1)

Publication using NB100-1029

• Newton JS, Deed RW, Mitchell EL et al. A B cell specific immediate early human gene is located on chromosome band 1q31 and encodes an alpha helical basic phosphoprotein. Biochim Biophys Acta 1993-11-16 [PMID: 8241276]

Bioinformatics

• Gene Symbol: RGS1
• Entrez:
  • 5996(Human)
  • 50778(Mouse)
• Uniprot:
  • NP_002913(Human)