RFC2 Antibody

Western Blot: RFC2 Antibody [H00005982-B02P] - Analysis of RFC2 expression in HL-60.

Immunocytochemistry/Immunofluorescence: RFC2 Antibody [H00005982-B02P] - Analysis of purified antibody to RFC2 on HeLa cell. (antibody concentration 10 ug/ml)

Immunohistochemistry-Paraffin: RFC2 Antibody [H00005982-B02P] - Analysis of purified antibody to RFC2 on formalin-fixed paraffin-embedded human lymph node. (antibody concentration 3 ug/ml)

Western Blot: RFC2 Antibody [H00005982-B02P] - Analysis of RFC2 expression in HeLa.

Western Blot: RFC2 Antibody [H00005982-B02P] - Analysis of RFC2 expression in transfected 293T cell line by RFC2 polyclonal antibody. Lane 1: RFC2 transfected lysate(35.2 KDa). Lane 2: Non-transfected lysate.

Product Discontinued

RFC2 Antibody Summary

• Description: Quality control test: Antibody reactive against mammalian transfected lysate.
• Immunogen: RFC2 (NP_002905.2, 1 a.a. - 320 a.a.) full-length human protein. MEVEAVCGGAGEVEAQDSDPAPAFSKAPGSAGHYELPWVEKYRPVKLNEIVGNEDTVSRLEVFAREGNVPNIIIAGPPGTGKTTSILCLARALLGPALKDAMLELNASNDSMTDGAQQALRRTMEIYSKTTRFALACNASDKIIEPIQSRCAVLRYTKLTDAQILTRLMNVIEKERVPYTDDGLEAIIFTAQGDMRQALNNLQSTFSGFGFINSENVFKVCDEPHPLLVKEMIQHCVNANIDEAYKILAHLWHLGYSPEDIIGNIFRVCKTFQMAEYLKLEFIKEIGYTHMKIAEGVNSLLQMAGLLARLCQKTMAPVAS
• Specificity: RFC2 - replication factor C (activator 1) 2, 40kDa,
• Isotype: IgG
• Clonality: Polyclonal
• Host: Mouse
• Gene: RFC2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
• Application Notes: Antibody reactivity against recombinant protein with GST tag on ELISA and WB and also on transfected lysate in WB. GST tag alone is used as a negative control. It has been used for IF and IHC-P.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Purity: Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for RFC2 Antibody

• A1 40 kDa subunit
• A1
• Activator 1 40 kDa subunit
• Activator 1 subunit 2
• MGC3665
• replication factor C (activator 1) 2, 40kDa
• replication factor C 2
• Replication factor C 40 kDa subunit
• replication factor C subunit 2
• RF-C 40 kDa subunit
• RFC40replication factor C (activator 1) 2 (40kD)

Background

The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins, proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also called activator 1, is a protein complex consisting of five distinct subunits of 145, 40, 38, 37, and 36.5 kD. This gene encodes the 40 kD subunit, which has been shown to be responsible for binding ATP. Deletion of this gene has been associated with Williams syndrome. Alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RFC2
• Entrez:
  • 5982(Human)
• Uniprot:
  • NP_002905.2(Human)