Rev-erb A alpha/NR1D1 Antibody

Western Blot: Rev-erb A alpha/NR1D1 Antibody [NBP2-19574] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Rev-erb A alpha/NR1D1 antibody (NBP2-19574) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: Rev-erb A alpha/NR1D1 Antibody [NBP2-19574] - A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NR1D1 protein stained by NR1D1 antibody diluted at 1:400. Blue: Hoechst 33342 staining.

Immunohistochemistry-Paraffin: Rev-erb A alpha/NR1D1 Antibody [NBP2-19574] - NR1D1 antibody detects NR1D1 protein at cytosol and nucleus on mouse kidney by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. NR1D1 antibody dilution: 1:500.

Immunohistochemistry-Paraffin: NR1D1 Antibody [NBP2-19574] - NR1D1 antibody detects NR1D1 protein at nucleus on U87 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded U87 xenograft. NR1D1 antibody dilution: 1:500.

• Reactivity: Hu, Mu, Rt, Bv, Sh
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

Rev-erb A alpha/NR1D1 Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human Rev-erb A alpha/NR1D1. The exact sequence is proprietary.
• Localization: Nucleus
• Predicted Species: Rat (93%), Sheep (92%), Bovine (91%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NR1D1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:10000
• Theoretical MW: 67 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 20% Glycerol, 1% BSA
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for Rev-erb A alpha/NR1D1 Antibody

• Ear-1
• EAR1nuclear receptor subfamily 1 group D member 1
• HREV
• NR1D1
• nuclear receptor subfamily 1, group D, member 1
• Reverb A alpha
• Rev-erb A alpha
• Rev-ErbAalpha
• Rev-erbA-alpha
• THRA1
• THRAL
• THRALrev-erbA-alpha
• V-erbA-related protein 1

Background

Functions as a constitutive transcriptional repressor. Possible receptor for triiodothyronine.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for Rev-erb A alpha/NR1D1 Antibody (NBP2-19574)(1)

Publication using NBP2-19574

• de Assis LVM, Moraes MN, Mendes D Et al. Loss of Melanopsin (OPN4) Leads to a Faster Cell Cycle Progression and Growth in Murine Melanocytes Current issues in molecular biology 2021-10-04 [PMID: 34698095] (FLOW, Mouse)

Bioinformatics

• Gene Symbol: NR1D1
• Uniprot:
  • P20393(Human)