Renalase Antibody

Western Blot: Renalase Antibody [NB100-93435] - (0.5ug/ml) staining of Rat Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Mu, Rt, Hu
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Renalase Antibody Summary

• Description: Novus Biologicals Goat Renalase Antibody (NB100-93435) is a polyclonal antibody validated for use in WB and ELISA. Anti-Renalase Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-QINLRDDKWEVSKQ corresponding to internal region according to NP_001026879.1, NP_060833.1.
• Epitope: C-QINLRDDKWEVSKQ
• Specificity: This antibody is expected to recognize both reported isoforms (NP_001026879.1 and NP_060833.1).
• Predicted Species: Human (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: RNLS
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.5 - 1.5 ug/ml
• Application Notes: WB: Approx. 37 kDa band observed in mouse kidney and rat kidney lysates (calculated MW of 37.8 kDa band according to human NP_001026879.1 and 35.0 kDa band according to rat NP_001014189.1).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Renalase Antibody

• C10orf59
• C10orf59chromosome 10 open reading frame 59
• EC 1.4
• FLJ11218
• MAO-C
• Monoamine oxidase-C
• Renalase
• renalase, FAD-dependent amine oxidase
• RNLS

Background

Renalase is a flavin adenine dinucleotide-dependent amine oxidase that is secreted into the blood from the kidney (Xu et al., 2005 [PubMed 15841207]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Renalase Antibody (NB100-93435)(1)

Publication using NB100-93435

• Xu J, Li G, Wang P et al. Renalase is a novel, soluble monoamine oxidase that regulates cardiac function and blood pressure. J Clin Invest 2005-05-02 [PMID: 15841207]

Bioinformatics

• Gene Symbol: RNLS
• Entrez:
  • 55328(Human)
  • 67795(Mouse)
  • 361751(Rat)
• Uniprot:
  • NP_001026879.1(Human)