Renalase Antibody

Images

 
Western Blot: Renalase Antibody [NB100-1493] - (0.1ug/ml) staining of Mouse Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry: Renalase Antibody [NB100-1493] - (3ug/ml) staining of paraffin embedded Human Kidney. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

Renalase Antibody Summary

Immunogen
Peptide with sequence C-VSIDNKKRNI corresponding to internal region according to NP_001026879.2, NP_060833.1.
Specificity
This antibody is expected to recognise both reported isoforms (NP_001026879.2 and NP_060833.1).
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
RNLS
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 - 0.3 ug/ml
  • Immunohistochemistry 2 - 4 ug/ml
  • Immunohistochemistry-Paraffin 2 - 4 ug/ml
  • Peptide ELISA Detection limit 1:64000
Application Notes
WB: Approx. 37 kDa band observed in human kidney and mouse kidney lysates (calculated MW of 37.8 kDa band according to NP_001026879.1 and 34.9 kDa band according to NP_060833.1). IHC-P: Human kidney shows vesicular staining in the epithelial cells of the CT.
Publications
Read Publication using NB100-1493.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Rat, Canine, Bovine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Renalase Antibody

  • C10orf59
  • C10orf59chromosome 10 open reading frame 59
  • EC 1.4
  • FLJ11218
  • MAO-C
  • Monoamine oxidase-C
  • Renalase
  • renalase, FAD-dependent amine oxidase
  • RNLS

Background

Renalase is a flavin adenine dinucleotide-dependent amine oxidase that is secreted into the blood from the kidney (Xu et al., 2005).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Renalase Antibody (NB100-1493)(1)

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Product General Protocols

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Video Protocols

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol RNLS
Entrez
Uniprot