Renalase Antibody [Unconjugated]

Western blot shows lysates of human plasma. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Renalase Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5350) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Renalase at approximately 37 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Identification of plasma membrane calcium ATPase isoform PMCA4b as a renalase binding protein.A, HK-2 cells incubated with either labeled RP-Scr220 or RP-220, biotin-labeled proteins purified using streptavidin column, separated by SDS-PAGE and visualized by western blot using streptavidin-HRP; * = regions evaluated by mass spectrometry in samples labeled with either RP-Scr220 or RP-220; # = RP-220 band containing the plasma membrane calcium ATPase isoform PMCA4b. B, Endogenous expression of PMCA4b in HK-2 cells, western immunoblot using isoform specific monoclonal; CCL-119: human leukemic cell line; thyroid tumor = human thyroid tumor cell line (ATCC, CRL-1803) 10 μg protein loaded in each lane. C, co-immunolocalization of PMCA4b and renalase in HK-2 cells, images acquired using a Zeiss laser scanning confocal microscope, scale bar = 9 μm; arrow = plasma membrane. D, Co-Immunoprecipitation of PMCA4b and renalase from HK-2 cell lysates; renalase-Ab-beads = renalase antibody coated beads; PMCA4b-Ab-beads = PMCA4b antibody coated beads. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25906147), licensed under a CC-BY license. Not internally tested by R&D Systems.

Identification of plasma membrane calcium ATPase isoform PMCA4b as a renalase binding protein.A, HK-2 cells incubated with either labeled RP-Scr220 or RP-220, biotin-labeled proteins purified using streptavidin column, separated by SDS-PAGE and visualized by western blot using streptavidin-HRP; * = regions evaluated by mass spectrometry in samples labeled with either RP-Scr220 or RP-220; # = RP-220 band containing the plasma membrane calcium ATPase isoform PMCA4b. B, Endogenous expression of PMCA4b in HK-2 cells, western immunoblot using isoform specific monoclonal; CCL-119: human leukemic cell line; thyroid tumor = human thyroid tumor cell line (ATCC, CRL-1803) 10 μg protein loaded in each lane. C, co-immunolocalization of PMCA4b and renalase in HK-2 cells, images acquired using a Zeiss laser scanning confocal microscope, scale bar = 9 μm; arrow = plasma membrane. D, Co-Immunoprecipitation of PMCA4b and renalase from HK-2 cell lysates; renalase-Ab-beads = renalase antibody coated beads; PMCA4b-Ab-beads = PMCA4b antibody coated beads. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25906147), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Renalase Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human Renalase
  Ala2-Ile342
  Accession # NP_001026879
• Specificity: Detects human Renalase in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: RNLS
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
  *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Renalase Antibody [Unconjugated]

• C10orf59
• C10orf59chromosome 10 open reading frame 59
• EC 1.4
• FLJ11218
• MAO-C
• Monoamine oxidase-C
• Renalase
• renalase, FAD-dependent amine oxidase
• RNLS

Background

Renalase is a novel, 35‑37 kDa FAD-dependent amine oxidase that is secreted primarily by glomerular and renal tubule epithelium. It presumably degrades catecholamines and lowers blood pressure by decreasing heart rate and cardiac contractility. In addition to renal cells, Renalase is produced by skeletal and cardiac muscle fibers. The human Renalase precursor is 342 amino acids (aa) in length. It contains an FAD binding domain (aa 4‑35) that overlaps a signal sequence (aa 1‑17), followed by an amine oxidase segment (aa 75‑339). Renalase circulates as both a monomer (from kidney) and homodimer (from heart). There is one potential splice variant that shows a 22 aa substitution for aa 294‑342. Over aa 2‑342, human Renalase shows 72% aa identity to mouse Renalase.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Renalase Antibody (AF5350)(1)

Publication using AF5350

• Wang , Ling, Velazquez , Heino, Chang , John, Safirstein , Robert, Desir , Gary V Identification of a receptor for extracellular renalase. PLoS ONE, 2015-04-23;10(4):e0122932. 2015-04-23 [PMID: 25906147] (ICC, Human)

Review for Renalase Antibody (AF5350) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer WB Human 01/08/2015

Summary

• Application: Western Blot
• Sample Tested: See PMID 23130169
• Species: Human

Bioinformatics

• Gene Symbol: RNLS
• Uniprot:
  • NP_001026879()