Recombinant Rat PDGF-AB Protein, CF

• Reactivity: Rt
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Rat PDGF-AB Protein, CF Summary

• Details of Functionality: Measured in a cell proliferation assay using NR6R‑3T3 mouse fibroblast cells. Raines, E.W. et al. (1985) Methods Enzymol. 109:749. The ED₅₀ for this effect is 1-3 ng/mL.
• Source: E. coli-derived rat PDGF-AB protein
  

  Rat PDGF-A (Ser87 - Arg196) Accession # AAB59693.1

  Rat PDGF-B (Ser74 - Thr182) Accession # Q05028.1
  N-terminus	C-terminus

• Accession #: AAB59693.1 (A chain) & Q05028.1 (B chain)
• N-terminal Sequence: Ser87 (A chain) & Ser74 (B chain)
• Structure / Form: Disulfide-linked heterodimer
• Protein/Peptide Type: Recombinant Proteins
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 24.8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in Acetonitrile and Na2HPO₄.
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile 4 mM HCl.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Rat PDGF-AB Protein, CF

• PDGFAB
• PDGF-AB

Background

Platelet-derived growth factor (PDGF) family of mesenchymal mitogenic factors consists of four homodimers (PDGF-AA, PDGF-BB, PDGF-CC and PDGF-DD) and one heterodimer (PDGF-AB). These proteins are expressed by a number of different cells and tissues including endothelial cells, epithelial cells, hematopoietic cells, connective tissue, nervous tissue, brain, muscle, kidney and liver. PDGF expression is highly regulated with PDGF levels increasing following injury and/or disease. An analysis of PDGF purified from human platelets suggest that approximately 70% of sera-derived PDGF is PDGF-AB (1).

PDGF isoforms exert their cellular effect by binding to the structurally similar receptor tyrosine kinases PDGF receptor-alpha and PDGF receptor-beta . PDGF-AB binding induces receptor dimerization resulting in alpha alpha receptor homodimers and alpha beta receptor heterodimers. Binding of PDGF receptors has been reported to result in stimulation of mitogenicity and chemotaxis of fibroblasts, stimulation of granule release by neutrophils and monocytes, facilitation of steroid synthesis by Leydig cells, stimulation of neutrophil phagocytosis, stimulation of collagen, fibronectin, proteoglycan, and hyaluronic acid synthesis, modulation of thrombospondin expression and secretion, stimulation of collagenase activity and secretion, induction of contraction of rat aorta strips in vitro, and transient induction of T cell IL-2 secretion accompanied by a down-regulation of IL-4 and IFN-gamma production (2).

1. Raines, E.W. (2001) in Cytokine Reference, J.J. Oppenheim and M. Feldmann, chief editors, Academic Press, San Diego, California, pp. 755.
2. Heldin, C-H, Eriksson, U. and Ostman, A. (2002) Arch. Biochem. Biophysic. 398:284.

Publications for PDGF-AB (1115-AB)(2)

Publications using 1115-AB

• JL Kadrmas, MC Beckerle, M Yoshigi Genetic analyses in mouse fibroblast and melanoma cells demonstrate novel roles for PDGF-AB ligand and PDGF receptor alpha Sci Rep, 2020-11-09;10(1):19303. 2020-11-09 [PMID: 33168840] (Bioassay, Rat)
• X Sun, Y Zhu, HY Yin, ZY Guo, F Xu, B Xiao, WL Jiang, WM Guo, HY Meng, SB Lu, Y Wang, J Peng Differentiation of adipose-derived stem cells into Schwann cell-like cells through intermittent induction: potential advantage of cellular transient memory function Stem Cell Res Ther, 2018-05-11;9(1):133. 2018-05-11 [PMID: 29751848] (Bioassay, Rat)

Bioinformatics

• Uniprot:
  • AAB59693.1 (A chain) & Q05028.1 (B chain)()