Recombinant Rat OX40 Ligand/TNFSF4 Protein

• Reactivity: Rt
• Applications: Bioactivity

Recombinant Rat OX40 Ligand/TNFSF4 Protein Summary

• Details of Functionality: Measured by its ability to co-stimulate IL-2 secretion by mouse T cells in the presence of anti-CD3. The ED₅₀ for this effect is 0.05-0.25 μg/mL in the presence of a cross-linking antibody, Mouse Anti-polyHistidine Monoclonal Antibody (Catalog # MAB050).
• Source: Mouse myeloma cell line, NS0-derived rat OX40 Ligand/TNFSF4 protein
  Gln49-Leu199, with an N-terminal 6-His tag
• Accession #: Q9Z2P3
• N-terminal Sequence: His
• Protein/Peptide Type: Recombinant Proteins
• Gene: Tnfsf4
• Purity: >95%, by SDS-PAGE with silver staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 18 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 17-31 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with BSA as a carrier protein.
• Purity: >95%, by SDS-PAGE with silver staining.
• Reconstitution Instructions: Reconstitute at 250 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Rat OX40 Ligand/TNFSF4 Protein

• CD134 ligand
• CD134L
• CD252 antigen
• CD252
• Glycoprotein Gp34
• gp34
• OX40 antigen ligand
• OX40 Ligand
• OX40L
• OX-40L
• OX4OL
• TAX transcriptionally-activated glycoprotein 1
• tax-transcriptionally activated glycoprotein 1 (34kD)
• tax-transcriptionally activated glycoprotein 1, 34kD
• TNFSF4
• tumor necrosis factor (ligand) superfamily, member 4
• tumor necrosis factor ligand superfamily member 4
• TXGP1

Background

OX40 Ligand (OX40L; also known as TNFSF4, CD252, and gp34) is a type II transmembrane glycoprotein belonging to the TNF superfamily (1-3). OX40 Ligand coordinates with other costimulators (B7, CD40 Ligand/TNFSF5, CD30 Ligand/TNFSF8, CD27 Ligand/TNFSF7, and 4-1BB Ligand/TNFSF9) to manage the activation of the immune response. The rat OX40 Ligand cDNA encodes a 199 amino acid (aa) polypeptide with a short amino-terminal cytoplasmic domain and a 151 aa carboxy-terminal extracellular domain (ECD). The rat OX40 Ligand ECD shares 79% and 40% aa sequence identity with mouse and human OX40 Ligand ECD, respectively. OX40 Ligand is expressed on the surface of activated antigen presenting cells, B cells, T cells, and endothelial cells at sites of inflammation (4-6). Similar to other TNF superfamily members, membrane-bound OX40 Ligand is expressed as a homotrimer (7). OX40 Ligand binds to the type 1 transmembrane receptor, OX40, which is expressed on activated T cells. OX40 Ligand-OX40 signaling results in increased T cell survival, proliferation, and cytokine production (8, 9). It also modulates natural killer cell function, inhibits the conversion of effector T cells into immunosuppressive regulatory T cells (Tregs), and promotes the maintenance of and recall response in memory T cells (7-13). OX40 Ligand-OX40 signaling is involved in allergic airway inflammation, graft-versus-host disease, and other autoimmune diseases (14-16). Mutations in OX40 and OX40 Ligand are associated with cardiovascular disease (13, 17).

1. Godfrey, W.R. et al. (1994) J. Exp. Med. 180:757.
2. al-Shamkhani, A. et al. (1996) Eur. J. Immunol. 26:1695.
3. Baum, P.R. et al. (1994) Circ. Shock 44:30.
4. Gough, M.J. and A.D. Weinberg (2009) Adv. Exp. Med. Biol. 647:94.
5. Moran, A.E. et al. (2013) Curr. Opin. Immunol. 25:230.
6. Ohshima, Y. et al. (1997) J. Immunol. 159:3838.
7. Croft, M. (2010) Annu. Rev. Immunol. 28:57.
8. So, T. and M. Croft (2007) J. Immunol. 179:1427.
9. Xiao, X. et al. (2008) J. Immunol. 181:3193.
10. Mousavi, S.F. et al. (2008) J. Immunol. 181:5990.
11. Salek-Ardakani, S. et al. (2003) J. Exp. Med. 198:315.
12. Bansal-Pakala, P. et al. (2001) Nat. Med. 7:907.
13. Ishii, N. et al. (2010) Adv. Immunol. 105:63.
14. Damayanti, T. et al. (2010) Am. J. Respir. Crit. Care Med. 181:688.
15. Xiao, X. et al. (2012) Nat. Immunol. 13:981.
16. Gramaglia, I. et al. (1998) J. Immunol. 161:6510.
17. Nakano, M. et al. (2010) Cardiovasc. Res. 88:539.

Bioinformatics

• Gene Symbol: Tnfsf4
• Uniprot:
  • Q9Z2P3()