Recombinant Rat MANF His-tag Protein, CF

2 μg/lane of Recombinant Rat MANF His-tag Protein (Catalog # 10430-MN) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 18-23 kDa and 13-18 kDa, respectively.

• Reactivity: Rt
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Rat MANF His-tag Protein, CF Summary

• Details of Functionality: Bioassay data are not available.
• Source: Mouse myeloma cell line, NS0-derived rat MANF protein
  Leu22-Leu179, with a C-terminal 6-His tag
• Accession #: P0C5H9.1
• N-terminal Sequence: Leu22
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 19 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 18-23 kDa, under reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 1 mg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Rat MANF His-tag Protein, CF

• Arginine-Rich Protein
• arginine-rich, mutated in early stage tumors
• ARMET
• ARP
• MANF
• mesencephalic astrocyte-derived neurotrophic factor
• Protein ARMET

Background

Mesencephalic astrocyte-derived neurotrophic factor (MANF), also known as arginine-rich, mutated in early stage tumors (ARMET) and arginine-rich protein (ARP), is a 20 kDa member of the ARMET family of proteins (1). The name ARMET comes from the fact that the protein was initially thought to be 50 amino acid (aa) longer at the N-terminus and to contain an arginine-rich region (2-5). The presence of a specific mutation changing the previously numbered codon 50 from ATG to AGG, or deletion of that codon, has been reported in a variety of solid tumors (2-4). Rat MANF is synthesized as a 179 aa precursor that contains a 21 aa signal sequence and a 158 aa mature chain. Mature rat MANF is 99% identical to mature human and mouse MANF. MANF is localized to the endoplasmic reticulum (ER) and Golgi apparatus, and is also secreted (5). In the CNS, MANF selectively protects nigral dopaminergic neurons, versus GABAergic or serotonergic neurons, which suggests that MANF may be indicated for the treatment of Parkinson's disease (1). MANF is also one of the 12 commonly unfolded protein response (UPR)-up-regulated genes (5). One study showed that MANF plays an important role in protecting cells against tunicamycin and thapsigargin-induced cell death (5). Loss of MANF renders cells more susceptible to those drugs, but also increases cell proliferation and decreases cell size (5). Another study showed that MANF is an endoplasmic reticulum stress response (ERSR) gene in the heart that can be induced and secreted in response to ER stresses, including ischemia, and that extracellular MANF may protect cardiac myocytes in an autocrine and paracrine manner (6).

1. Petrova, P.S. et al. (2003) J. Mol. Neurosci. 20:173.
2. Shridhar, V. et al. (1996) Oncogene 12:1931.
3. Shridhar, R. et al. (1996) Cancer Res. 56:5576.
4. Shridhar, V. et al. (1997) Oncogene 14:2213.
5. Apostolou, A. et al. (2008) Exp. Cell Res. 314:2454.
6. Tadimalla, A. et al. (2008) Circ. Res. 103:1249.

Bioinformatics

• Uniprot:
  • P0C5H9.1()