Recombinant Rat LIX Protein

Images

 
There are currently no images for LIX (543-RL).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity RtSpecies Glossary
Applications Bioactivity

Order Details

View Available Formulations
Catalog# & Formulation Size Price

Recombinant Rat LIX Protein Summary

Details of Functionality
Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CXCR2. The ED50 for this effect is 0.03-0.15 µg/mL.
Source
E. coli-derived rat LIX protein
Ala38-Arg125 & Ala38-Lys117
Accession #
N-terminal Sequence
Ala38
Protein/Peptide Type
Recombinant Proteins
Gene
Cxcl6
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
10.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
543-RL in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 25 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Rat LIX Protein

  • LIX

Background

LPS-induced CXC chemokine (LIX) is a chemokine originally cloned from LPS-stimulated mouse fibroblasts. Rat LIX shares approximately 74% amino acid (aa) sequence identity with mouse LIX and is likely the rat orthologue to mouse LIX. Rat LIX cDNA encodes a 130 aa residue precursor with a predicted 37 aa residue signal peptide and a 93 aa residue mature protein. Among human CXC chemokines, rat LIX is most closely related to human GCP-2 and ENA-78. Rat LIX also differs from these two human proteins by having an extended carboxy-terminus. The amino-terminal 115 residues of rat LIX shares 59% and 53% aa sequence homology with human GCP-2 and ENA-78, respectively. It is not clear if LIX should be considered an orthologue of GCP-2 or ENA-78. Yet, mouse LIX was alternatively named mouse GCP-2.

Purified recombinant rat LIX is C-terminally truncated. This is consistent with the observation that natural mouse LIX purified from fibroblasts and epithelial cells also contains multiple amino-terminal and carboxy-terminal truncated isoforms. The shorter isoforms of the natural protein were reported to be more active than the longer forms. The bioactivity of rat LIX can be mediated through CXCR2.

  1. Proost, P. et al. (1993) J. Immunol. 150:1000.
  2. Smith, J.B. and H.R. Herschman (1995) J. Biol. Chem. 270:16756.
  3. Rovai, L.E. et al. (1997) J. Immunol. 158:5257.
  4. Wuyts, A. et al. (1997) J. Immunol. 157:1736.

Customers Who Viewed This Item Also Viewed...

MAB208
Species: Hu
Applications: WB, Simple Western, CyTOF-ready, ELISA(Cap), ELISA(Det), ICC, ICFlow, Neut, ELISA(Sta)
MAB333
Species: Hu
Applications: WB, ELISA(Cap), ELISA(Det), Neut, ELISA(Sta)
MAB331
Species: Hu
Applications: Flow, IHC, CyTOF-ready, Neut
NBP2-53097
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
MAB330
Species: Hu
Applications: Flow, IHC, CyTOF-reported, Neut
543-RL
Species: Rt
Applications: Bioactivity

Publications for LIX (543-RL)(2)

We have publications tested in 2 confirmed species: Rat, N/A.

We have publications tested in 2 applications: Bioassay, ELISA Developmet.


Filter By Application
Bioassay
(1)
ELISA Developmet
(1)
All Applications
Filter By Species
Rat
(1)
N/A
(1)
All Species

Reviews for LIX (543-RL) (0)

There are no reviews for LIX (543-RL). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for LIX (543-RL) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Rat LIX Protein and receive a gift card or discount.

Bioinformatics

Gene Symbol Cxcl6
Uniprot