Recombinant Rat IL-9R Protein

• Reactivity: Rt
• Applications: Bioactivity

Recombinant Rat IL-9R Protein Summary

• Details of Functionality: Measured by its ability to inhibit IL-9-induced proliferation of TS1 mouse helper T cells. The ED₅₀ for this effect is 0.25-1.25 µg/mL in the presence of rrIL-9 at 50 ng/mL.
• Source: Mouse myeloma cell line, NS0-derived rat IL-9 R protein
  Val38-Ala270, with a C-terminal 6-His tag
• Accession #: EDM04022
• N-terminal Sequence: Val38
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 27.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 33-40 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 200 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Rat IL-9R Protein

• CD129 antigen
• CD129
• IL-9 R
• IL-9 receptor
• IL9R
• IL-9R
• interleukin 9 receptor
• interleukin-9 receptor

Background

The IL-9 receptor alpha protein is a member of the type I cytokine receptor family. It is a 62 kDa IL-9 receptor subunit (previously Gfi-2, designated CD129) that binds IL-9 and pairs with the 64 kDa cytokine receptor common gamma -chain to allow cell signaling (1 - 3). The 467 amino acid (aa) rat IL-9 R alpha precursor is predicted to contain a 37 aa signal sequence, a 232 aa extracellular domain (ECD) with four conserved cysteine residues in its N-terminal region, a fibronectin type III domain and a WSXWS motif, a 21 aa transmembrane (TM) domain, and a 177 aa cytoplasmic domain with a Box 1 JAK-binding motif (1, 2, 4). A potential isoform contains a 36 aa sequence that is substituted for the TM and cytoplasmic domains (5). The ECD of rat IL-9 R alpha shares 86%, 63%, 66%, 63% and 59% aa identity with the ECD of mouse, human, equine, canine and bovine IL-9 R alpha , respectively. Mast cells, germinal center and B-1b B cells, T cell blasts, and myeloid progenitors express IL-9 R (1, 6 - 8). The primary functions of IL-9 have been difficult to determine due to the cross-functionality and cross-induction of cytokines. IL-9 production by regulatory T cells (T-regs), however, is crucial for mast cell recruitment and differentiation, which in turn is crucial for tolerance of allografts (7). Genetic deletion and transgenic expression of IL-9 and other cytokines has also demonstrated the role of IL-9 in mastocytosis, eosinophilia and B cell infiltration of the lung in a mouse asthma-like model, while IL-9 induction of IL-13 is responsible for eosinophil recruitment and mucous upregulation (8 - 10).

1. Knoops, L. and J-C. Renauld (2004) Growth Factors 22:207.
2. Flubacher, M.M. et al. (1994) J. Virol. 68:7709.
3. Demoulin, J-B. et al. (1996) Mol. Cell. Biol. 16:4710.
4. Swissprot Accession # Q63216
5. Entrez Accession # EDM04023
6. Fawaz, L.M. et al. (2007) J. Allergy Clin. Immunol. 120:1208.
7. Lu, L-F. et al. (2006) Nature 442:997.
8. Steenwinckel, V. et al. (2007) J. Immunol. 178:3244.
9. Townsend, M.J. et al. (2000) Immunity 13:573.
10. Fallon, P.G. et al. (2002) Immunity 17:7.

Bioinformatics

• Uniprot:
  • EDM04022()