Recombinant Rat IL-1ra/IL-1F3 Protein

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Summary
Reactivity RtSpecies Glossary
Applications Bioactivity

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Recombinant Rat IL-1ra/IL-1F3 Protein Summary

Details of Functionality
Measured by its ability to inhibit IL-1 alpha -dependent proliferation in D10.G4.1 mouse helper T cells. Symons, J.A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 272. The ED50 for this effect is 38-150 ng/mL in the presence of 50 pg/mL of rrIL-1 alpha.
Source
E. coli-derived rat IL-1ra/IL-1F3 protein
His27-Gln178, with an N-terminal Met
Accession #
N-terminal Sequence
Met
Protein/Peptide Type
Recombinant Proteins
Gene
Il1rn
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
17.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
1545-RA in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS and DTT with BSA as a carrier protein.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Rat IL-1ra/IL-1F3 Protein

  • DIRA
  • ICIL-1ra
  • IL1F3
  • IL-1F3
  • IL1ra
  • IL-1ra
  • IL-1ra3
  • IL1RN
  • IL-1RN
  • interleukin 1 receptor antagonist
  • IRAP
  • MVCD4

Background

Interleukin 1 alpha (IL-1 alpha ), IL-1 beta, and IL-1 receptor antagonist (IL-1ra) are members of the IL-1 family. IL-1 alpha and IL-1 beta are agonists that bind the functional IL-1 receptor I (IL-1 RI), which then recruits the IL-1 receptor accessory protein (IL-1 R AcP/IL-1 R3) and initiates signal transduction pathways that include the NF-kappa B. JNK/AP-1, and p38 MAP kinase pathways. Both IL-1 alpha and IL-1 beta also bind the non‑signaling IL-1 RII that functions as a decoy receptor. IL-1ra is a receptor antagonist that binds both IL-1 RI and II with equal affinity as the IL-1 alpha and beta. Binding of IL-1ra to IL-1 RI does not initiate signal transduction pathways. IL-1 alpha and beta are pro‑inflammatory cytokines that play an important role in host defense. The imbalance between IL-1 ( alpha  and  beta ) and IL-1ra can influence the development of various pathologic conditions associated with chronic inflammation and tissue destruction. Pre‑clinical and clinical studies have indicated that IL-1ra may have therapeutic potential for the treatment of sepsis, rheumatoid arthritis and chronic myelogenous leukemia (1, 2).

Rat IL-1ra cDNA encodes a 178 amino acid (aa) residues secreted protein with a 26 aa signal peptide. Although intracellular isoforms of human IL-1ra that are transcribed from the same gene using different promotors have been described, analogous rat isoforms have not been reported. Rat IL-1ra shares 89% and 73% aa sequence homology with mouse and human IL-1ra, respectively. Secreted IL-1ra has been shown to be expressed by monocytes, macrophages, neutrophils, fibroblasts and hepatocytes (1, 2).

  1. Dinarello, C.A. (1996) Blood 87:2095 (review).
  2. Arend, W.P. (2002) Growth Factor Reviews 23:323 (review).

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Publications for IL-1ra/IL-1F3/IL1RN (1545-RA)(12)

We have publications tested in 1 confirmed species: Rat.

We have publications tested in 2 applications: Bioassay, In Vivo.


Filter By Application
Bioassay
(4)
In Vivo
(8)
All Applications
Filter By Species
Rat
(12)
All Species
Showing Publications 1 - 10 of 12. Show All 12 Publications.
Publications using 1545-RA Applications Species
JJ Sanchez, JE Sanchez, S Noor, CD Ruffaner-H, S Davies, CR Wagner, LL Jantzie, N Mellios, DD Savage, ED Milligan Targeting the ?2-integrin LFA-1, reduces adverse neuroimmune actions in neuropathic susceptibility caused by prenatal alcohol exposure Acta Neuropathol Commun, 2019;7(1):54. 2019 [PMID: 30961664] (In Vivo, Rat) In Vivo Rat
L Taoro-Gonz, YM Arenas, A Cabrera-Pa, V Felipo Hyperammonemia alters membrane expression of GluA1 and GluA2 subunits of AMPA receptors in hippocampus by enhancing activation of the IL-1 receptor: underlying mechanisms J Neuroinflammation, 2018;15(1):36. 2018 [PMID: 29422059] (Bioassay, Rat) Bioassay Rat
Chentouf M, Guzman C, Hamze M, Gross R, Lajoix A, Peraldi-Roux S Possible protective effect of membrane lipid rafts against interleukin-1beta-mediated anti-proliferative effect in INS-1 cells. PLoS ONE, 2014;9(7):e102889. 2014 [PMID: 25068701] (Bioassay, Rat) Bioassay Rat
Lim G, Kim H, McCabe M, Chou C, Wang S, Chen L, Marota J, Blood A, Breiter H, Mao J A leptin-mediated central mechanism in analgesia-enhanced opioid reward in rats. J Neurosci, 2014;34(29):9779-88. 2014 [PMID: 25031415] (In Vivo, Rat) In Vivo Rat
Yamato M, Tamura Y, Eguchi A, Kume S, Miyashige Y, Nakano M, Watanabe Y, Kataoka Y Brain interleukin-1beta and the intrinsic receptor antagonist control peripheral Toll-like receptor 3-mediated suppression of spontaneous activity in rats. PLoS ONE, 2014;9(3):e90950. 2014 [PMID: 24621600] (In Vivo, Rat) In Vivo Rat
Sato K, Horiuchi Y, Jin Y, Malchinkhuu E, Komachi M, Kondo T, Okajima F Unmasking of LPA1 receptor-mediated migration response to lysophosphatidic acid by interleukin-1beta-induced attenuation of Rho signaling pathways in rat astrocytes. J. Neurochem., 2011;117(1):164-74. 2011 [PMID: 21244430] (Bioassay, Rat) Bioassay Rat
Lapierre DM, Tanabe N, Pereverzev A Lysophosphatidic acid signals through multiple receptors in osteoclasts to elevate cytosolic calcium concentration, evoke retraction, and promote cell survival. J. Biol. Chem., 2010;285(33):25792-801. 2010 [PMID: 20551326] (Bioassay, Rat) Bioassay Rat
Taepavarapruk P, Song C, Reductions of acetylcholine release and nerve growth factor expression are correlated with memory impairment induced by interleukin-1beta administrations: effects of omega-3 fatty acid EPA treatment. J. Neurochem., 2010;112(4):1054-64. 2010 [PMID: 19968753] (In Vivo, Rat) In Vivo Rat
Marchi N, Fan Q, Ghosh C, Fazio V, Bertolini F, Betto G, Batra A, Carlton E, Najm I, Granata T, Janigro D Antagonism of peripheral inflammation reduces the severity of status epilepticus. Neurobiol. Dis., 2009;33(2):171-81. 2009 [PMID: 19010416] (In Vivo, Rat) In Vivo Rat
Koo JW, Duman RS IL-1beta is an essential mediator of the antineurogenic and anhedonic effects of stress. Proc. Natl. Acad. Sci. U.S.A., 2008;105(2):751-6. 2008 [PMID: 18178625] (In Vivo, Rat) In Vivo Rat
Show All 12 Publications.

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Bioinformatics

Gene Symbol Il1rn
Uniprot