Recombinant Mouse TWEAK/TNFSF12 Protein, CF

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse TWEAK/TNFSF12 Protein, CF Summary

• Details of Functionality: Measured in a cell proliferation assay using HUVEC human umbilical vein endothelial cells. The ED₅₀ for this effect is 0.03-0.3 µg/mL.
• Source: E. coli-derived mouse TWEAK/TNFSF12 protein
  Arg105-His249, with an N-terminal Met and a 6-His tag
• Accession #: O54907.2
• N-terminal Sequence: Met
• Protein/Peptide Type: Recombinant Proteins
• Gene: Tnfsf12
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 17 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS and NaCl.
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse TWEAK/TNFSF12 Protein, CF

• APO3 ligand
• APO3LMGC20669
• DR3LGTWEAKAPO3/DR3 ligand
• MGC129581
• TNF-related weak inducer of apoptosis
• TNFSF12
• tumor necrosis factor (ligand) superfamily, member 12
• tumor necrosis factor ligand superfamily member 12
• TWEAK

Background

TNF-related weak inducer of apoptosis (TWEAK) is a type II transmembrane protein belonging to the TNF superfamily and has been designated TNFSF12. Mouse TWEAK is a 249 amino acid (aa) protein with an N-terminal 21 aa cytoplasmic domain, a 21 aa transmembrane region and a 204 aa C-terminal extracellular domain (1). The primary structures of the extracellular domains of human and mouse TWEAK are 88% identical. A soluble form of TWEAK is generated from the
membrane-associated molecules by proteolytic cleavage suggesting that TWEAK may have long-range effects. TWEAK is expressed widely in many tissues and cells (1). Although TWEAK has been proposed as a ligand that signals through the death domain receptor 3 (DR3) (2), a TNF receptor superfamily member currently designated TNFRSF25, subsequent studies did not demonstrate binding of TWEAK to cell lines that express DR3 (3). In cells that lack DR3, TWEAK has been shown to bind TWEAK receptor (TWEAK R), a novel TNF receptor superfamily member designated TNFRSF12A (4-7). TWEAK R, also known as fibroblast growth
factor-inducible 14 (Fn14), is a growth factor-inducible immediate-early response gene that is expressed in fibroblasts, hepatocellular carcinomas and endothelial cells. TWEAK-TWEAK R interaction has been shown to promote NF-kappa B activation and mediate multiple cell death pathways. On endothelial cells, TWEAK R plays a role in endothelial cell growth and migration. This effect of TWEAK is not due to up-regulation of VEGF (8).

1. Chicheportiche, Y. et al. (1997) J. Biol Chem. 272:32401.
2. Marsters, S. et al. (1998) Current Biol. 8:525.
3. Kaptein, A. et al. (2000) FEBS Letters, 485:135.
4. Schneider, P. et al. (1999) Eur. J. Immunol. 29:1785.
5. Nakayama, M. et al. (2002) J. Immunol. 168:734.
6. Feng, S.L. et al. (2000) Am. J. Pathol. 156:1253.
7. Wiley, S.R. et al. (2001) Immunity 15:837.
8. Lynch, C. et al. (1998) J. Biol. Chem. 274:8455.

Publications for TWEAK/TNFSF12 (1237-TW/CF)(14)

Publications using 1237-TW/CF

• AJ Vesting, A Jais, P Klemm, L Steuernage, P Wienand, M Fog-Tonnes, H Hvid, AL Schumacher, C Kukat, H Nolte, T Georgomano, J Altmüller, M Pasparakis, A Schmidt, M Krüger, MS Supprian, A Waisman, BK Straub, N Raschzok, M Bernier, AL Birkenfeld, N Hövelmeyer, JC Brüning, FT Wunderlich NIK/MAP3K14 in hepatocytes orchestrates NASH to hepatocellular carcinoma progression via JAK2/STAT5 inhibition Molecular Metabolism, 2022-11-07;66(0):101626. 2022-11-07 [PMID: 36356831] (Bioassay, Mouse)
• J Chen, F Jia, K Ren, M Luo, X Min, P Wang, S Xiao, Y Xia Inhibition of suppressor of cytokine signaling 1 mediates the profibrotic effect of TWEAK/Fn14 signaling on kidney cells Cell. Signal., 2020-03-23;71(0):109615. 2020-03-23 [PMID: 32217132] (Cell Culture, Mouse)
• S Daniela da, B Xu, M Maschietto, FA Marchi, MI Alkailani, K Bijian, D Xiao, MA Alaoui-Jam TRAF2 COOPERATES WITH FOCAL ADHESION SIGNALING TO REGULATE CANCER CELL SUSCEPTIBILITY TO ANOIKIS Mol. Cancer Ther., 2018-10-29;0(0):. 2018-10-29 [PMID: 30373932] (Bioassay, Mouse)
• L Peng, Q Li, H Wang, J Wu, C Li, Y Liu, J Liu, L Xia, Y Xia Fn14 deficiency ameliorates psoriasis-like skin disease in a murine model Cell Death Dis, 2018-07-23;9(8):801. 2018-07-23 [PMID: 30038329] (In Vivo, Mouse)
• L Hao, M Ren, B Rong, F Xie, MJ Lin, YC Zhao, X Yue, WQ Han, JQ Zhong TWEAK/Fn14 mediates atrial-derived HL-1 myocytes hypertrophy via JAK2/STAT3 signalling pathway J. Cell. Mol. Med., 2018-07-04;0(0):. 2018-07-04 [PMID: 29971943] (Bioassay, Mouse)
• HD Brightbill, E Suto, N Blaquiere, N Ramamoorth, S Sujatha-Bh, EB Gogol, GM Castanedo, BT Jackson, YC Kwon, S Haller, J Lesch, K Bents, C Everett, PB Kohli, S Linge, L Christian, K Barrett, A Jaochico, LM Berezhkovs, PW Fan, Z Modrusan, K Veliz, MJ Townsend, J DeVoss, AR Johnson, R Godemann, WP Lee, CD Austin, BS McKenzie, JA Hackney, JJ Crawford, ST Staben, MH Alaoui Ism, LC Wu, N Ghilardi NF-?B inducing kinase is a therapeutic target for systemic lupus erythematosus Nat Commun, 2018-01-12;9(1):179. 2018-01-12 [PMID: 29330524] (Bioassay, Mouse)
• V Fernández-, C Sastre, N Méndez-Bar, J Egido, JL Martín-Ven, C Gómez-Guer, LM Blanco-Col TWEAK blockade decreases atherosclerotic lesion size and progression through suppression of STAT1 signaling in diabetic mice Sci Rep, 2017-04-27;7(0):46679. 2017-04-27 [PMID: 28447667] (Bioassay, Mouse)
• Sasaki Y, Shimizu Y, Suzuki Y, Horikoshi S, Tomino Y TWEAK/Fn14 system and crescent formation in IgA nephropathy. BMC Nephrol, 2015-03-14;16(0):27. 2015-03-14 [PMID: 25885587] (Bioassay, Mouse)
• Al-Sawaf O, Fragoulis A, Rosen C, Kan Y, Sonmez T, Pufe T, Wruck C Nrf2 protects against TWEAK-mediated skeletal muscle wasting. Sci Rep, 2014-01-10;4(0):3625. 2014-01-10 [PMID: 24406502] (Bioassay, Mouse)
• Regulation of innate CD8+ T-cell activation mediated by cytokines. Proc Natl Acad Sci U S A, 2012-06-04;109(25):9971-6. 2012-06-04 [PMID: 22665806] (Bioassay, Mouse)
• Kayamuro H, Yoshioka Y, Abe Y, Katayama K, Yoshida T, Yamashita K, Yoshikawa T, Hiroi T, Itoh N, Kawai Y, Mayumi T, Kamada H, Tsunoda S, Tsutsumi Y TNF superfamily member, TL1A, is a potential mucosal vaccine adjuvant. Biochem. Biophys. Res. Commun., 2009-05-04;384(3):296-300. 2009-05-04 [PMID: 19406102] (In Vivo, Mouse)
• Dogra C, Changotra H, Wedhas N, Qin X, Wergedal JE, Kumar A TNF-related weak inducer of apoptosis (TWEAK) is a potent skeletal muscle-wasting cytokine. FASEB J., 2007-02-21;21(8):1857-69. 2007-02-21 [PMID: 17314137] (Bioassay, Mouse)
• Ando T, Ichikawa J, Wako M, Hatsushika K, Watanabe Y, Sakuma M, Tasaka K, Ogawa H, Hamada Y, Yagita H, Nakao A TWEAK/Fn14 interaction regulates RANTES production, BMP-2-induced differentiation, and RANKL expression in mouse osteoblastic MC3T3-E1 cells. Arthritis Res. Ther., 2006-01-01;8(5):R146. 2006-01-01 [PMID: 16945157] (Bioassay, Mouse)
• Polavarapu R, Gongora MC, Winkles JA, Yepes M Tumor necrosis factor-like weak inducer of apoptosis increases the permeability of the neurovascular unit through nuclear factor-kappa B pathway activation. J. Neurosci., 2005-11-02;25(44):10094-100. 2005-11-02 [PMID: 16267216] (In Vivo, Mouse)

Bioinformatics

• Gene Symbol: Tnfsf12
• Uniprot:
  • O54907.2()