Recombinant Mouse TSH alpha/beta Heterodimer Protein

• Reactivity: Mu
• Applications: Bioactivity

Recombinant Mouse TSH alpha/beta Heterodimer Protein Summary

• Details of Functionality: Measured by its ability to induce cAMP accumulation in HEK293 human embryonic kidney cells transfected with human TSH R. Morgenthaler, N.G. et al. (1998) Horm. Metab. Res. 30:162. The ED₅₀ for this effect is 0.015-0.15 ng/mL.
• Source: Chinese Hamster Ovary cell line, CHO-derived mouse TSH alpha/beta Heterodimer protein
  

  Mouse TSH beta (Phe21-Val138) Accession # P12656	(GGGS)3	Mouse TSH alpha (Leu25-Ser120) Accession # P01216
  N-terminus		C-terminus

• N-terminal Sequence: Phe21 (mTSH beta )
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE with silver staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 25 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 35-41 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Purity: >95%, by SDS-PAGE with silver staining.
• Reconstitution Instructions: Reconstitute at 100 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse TSH alpha/beta Heterodimer Protein

• thyroid stimulating hormone beta
• Thyrotropin
• TSH alpha/beta Heterodimer
• TSHB
• TSH-B
• TSH-BETA

Background

Thyroid Stimulating Hormone (TSH), also known as Thyrotropin, is a 28 kDa heterodimer belonging to the glycoprotein hormone family. It is composed of noncovalently linked glycosylated alpha and beta  chains. The alpha  subunit (CG alpha ) is also a component of Follicle-Stimulating Hormone, Luteinizing Hormone, and Chorionic Gonadotropin. The unique beta  subunit confers the protein’s specific biological action and is responsible for the interaction with its receptor (1-3). The approximately 14 kDa mouse CG alpha subunit shares 73% and 97% amino acid (aa) sequence identity with the human and rat orthologs, respectively. The approximately 15 kDa mouse TSH beta subunit shares 89% and 92% aa sequence identity with the human and rat orthologs, respectively. Multiple isoforms of TSH with differing bioactivity and
half-lives exist due to differences in the post-translational glycosylation and sialylation modifications of its subunits (1, 4). TSH is produced and secreted by the anterior pituitary gland. Its secretion is controlled by Thyrotropin-Releasing Hormone (TRH) from the hypothalamus. TSH travels to the thyroid gland and binds to the TSH Receptor to stimulate production of Thyroxine (T4), which is converted in peripheral tissues to Triiodothyronine (T3), a more biologically potent form of the thyroid hormone (1, 4, 5). TSH secretion is also controlled by plasma T3 and T4 via a negative feedback mechanism and by the neurotransmitters dopamine and Somatostatin (4, 5). Serum TSH levels are often assessed to evaluate thyroid function (4, 5). TSH has also been shown to be produced by bone marrow and is believed to have a direct effect on skeletal homeostasis by promoting bone strength and quality (6-9). Additionally, serum TSH levels have been shown to be decreased in the elderly (10).

1. Szkudlinshi, M.W. et al. (2002) Physiol. Rev. 82:473.
2. Farid, N.R. and M.W. Szkudlinski (2004) Endocrinology 145:4048.
3. Cahoreau, C. et al. (2015) Front. Endocrinol. (Lausanne) 6:26.
4. Roelfsema, F. and J.D. Veldhuis (2013) Endocr. Rev. 34:619.
5. Winter, W.E. and M.R. Signorino (2001) Ann. Clin. Lab. Sci. 31:221.
6. Klein, J.R. and H.C. Wang (2004) J. Exp. Biol. 207:55.
7. Abe, E. et al. (2003) Cell 115:151.
8. Sun, L. et al. (2006) Ann. N.Y. Acad. Sci. 1068:309.
9. Sendak, R.A. et al. (2007) Int. Orthop. 31:753.
10. Peeters, R.P. (2008) Hormones 7:28.

Publications for TSH alpha/beta (8885-TH)(1)

Publication using 8885-TH

• S Agarwal, KH Koh, NJ Tardi, C Chen, RR Dande, JP WerneckdeC, YR Sudhini, C Luongo, D Salvatore, B Samelko, MM Altintas, S Mangos, A Bianco, J Reiser Deiodinase-3 is a thyrostat to regulate podocyte homeostasis EBioMedicine, 2021-10-11;72(0):103617. 2021-10-11 [PMID: 34649077] (Bioassay, Mouse)