Recombinant Mouse Siglec-1 Fc Chimera Protein, CF

Images

 
There are currently no images for Siglec-1/CD169 (5610-SL).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Recombinant Mouse Siglec-1 Fc Chimera Protein, CF Summary

Details of Functionality
Measured by the ability of the immobilized protein to support the adhesion of human red blood cells. Kelm, S. et al. (1994) Current Biology 4:965. The ED50 for this effect is 0.6-3 μg/mL.
Source
Mouse myeloma cell line, NS0-derived mouse Siglec-1/CD169 protein
Mouse Siglec-1
(Thr20 - Arg1639)
Accession # CAM18034
IEGRMDP Mouse IgG2a
(Glu98 - Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Thr20
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
Siglec1
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
201.7 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
190-205 kDa under reducing conditions
Publications
Read Publications using
5610-SL in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Siglec-1 Fc Chimera Protein, CF

  • CD169
  • FLJ00051
  • sialic acid binding Ig-like lectin 1, sialoadhesin
  • sialoadhesin
  • Siglec1
  • Siglec-1

Background

Siglecs are sialic acid specific I-type lectins that belong to the immunoglobulin superfamily. Structurally, they are transmembrane proteins with an N-terminal Ig-like V-set domain followed by varying numbers of Ig-like C2-set domains (1, 2). Mouse Siglec-1, also known as sialoadhesin and CD169, is a 175 - 185 kDa glycoprotein that consists of a 1619 amino acid (aa) extracellular domain (ECD) with one Ig-like V-set domain and 16 Ig‑like C2-set domains, a 21 aa transmembrane segment, and a 35 aa cytoplasmic domain (3, 4). Within the ECD, mouse Siglec-1 shares 73% and 83% aa sequence identity with human and rat Siglec-1, respectively. Alternate splicing generates a soluble form of the ECD and a soluble isoform that is truncated following the first three Ig-like domains (3). Siglec-1 expression is restricted to lymph node and spleen macrophages and some tissue macrophages (4). The adhesive function of Siglec-1 is supported by the N-terminal Ig-like domain which shows a selectivity for alpha -2,3-linked sialic acid residues (4 - 6). Siglec-1 binds a number of sialylated molecules including the mannose receptor, MGL1, MUC1, PSGL-1, and different glycoforms of CD43 (7 - 10). Its binding capacity can be masked by endogenous sialylated molecules (11, 12). The sialylated and sulfated N-linked carbohydrates that modify Siglec-1 itself are required for ligand binding (7, 8). Siglec-1 is expressed on dendritic cells following rhinovirus exposure, and these DC promote T cell anergy (13). It is also induced on circulating monocytes during systemic sclerosis and HIV-1 infection (14 - 16). Siglec-1 can trap HIV-1 particles for trans infection of permissive cells (15).
  1. Varki, A. and T. Angata (2006) Glycobiology 16:1R.
  2. Crocker, P.R. et al. (2007) Nat. Rev. Immunol. 7:255.
  3. Crocker, P.R. et al. (1994) EMBO J. 13:4490.
  4. Hartnell, A. et al. (2001) Blood 97:288.
  5. Nath, D. et al. (1995) J. Biol. Chem. 270:26184.
  6. Crocker, P.R. et al. (1991) EMBO J. 10:1661.
  7. Martinez-Pomares, L. et al. (1999) J. Biol. Chem. 274:35211.
  8. Kumamoto, Y. et al. (2004) J. Biol. Chem. 279:49274.
  9. Nath, D. et al. (1999) Immunology 98:213.
  10. van den Berg, T.K. et al. (2001) J. Immunol. 166:3637.
  11. Nakamura, K. et al. (2002) Glycobiology 12:209.
  12. Barnes, Y.C. et al. (1999) Blood 93:1245.
  13. 13. Kirchberger, S. et al. (2005) J. Immunol. 175:1145.
  14. 14. York, M.R. et al. (2007) Arthritis Rheum. 56:1010.
  15. 15. Rempel, H. et al. (2008) PloS ONE 3:e1967.
  16. 16. van der Kuyl, A.C. et al. (2007) Plos ONE 2:e257.

Customers Who Viewed This Item Also Viewed...

H00003347-M01
Species: Hu
Applications: WB, ELISA, ICC/IF, S-ELISA
AF1107
Species: Mu
Applications: WB, IHC
NB300-109
Species: Hu, Mu, Rt, Dr, I, Ma
Applications: WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P, Dual ISH-IHC, IHC-FrFl, IHC-WhMt, KO
NBP2-79843
Species: Hu
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P, PA, CyTOF-ready
NB100-524
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Flow-IC
NBP1-88220
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P, KD
MAB5376
Species: Mu
Applications: WB, Flow, CyTOF-ready
NBP2-67605
Species: Hu
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP
NBP2-98675
Species: Hu
Applications: WB, IHC, IHC-P, IP
NB100-1598
Species: Hu, Mu
Applications: WB, Flow, ICC/IF
NBP1-69571
Species: Hu, Mu
Applications: WB, IHC, IHC-P
NBP1-87581
Species: Hu
Applications: WB, IHC, IHC-P
NB200-541
Species: Mu
Applications: WB, ICC/IF, IHC, IHC-Fr, IP

Publications for Siglec-1/CD169 (5610-SL)(2)

Reviews for Siglec-1/CD169 (5610-SL) (0)

There are no reviews for Siglec-1/CD169 (5610-SL). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for Siglec-1/CD169 (5610-SL) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Other Available Formats

Additional Siglec-1/CD169 Products

Bioinformatics Tool for Siglec-1/CD169 (5610-SL)

Discover related pathways, diseases and genes to Siglec-1/CD169 (5610-SL). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Siglec-1/CD169 (5610-SL)

Discover more about diseases related to Siglec-1/CD169 (5610-SL).
 

Pathways for Siglec-1/CD169 (5610-SL)

View related products by pathway.

PTMs for Siglec-1/CD169 (5610-SL)

Learn more about PTMs related to Siglec-1/CD169 (5610-SL).

Blogs on Siglec-1/CD169

There are no specific blogs for Siglec-1/CD169, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Mouse Siglec-1 Fc Chimera Protein, CF and receive a gift card or discount.

Bioinformatics

Gene Symbol Siglec1
Uniprot