Recombinant Mouse sFRP-2 Protein, CF

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications Binding Activity
Format
Carrier-Free

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Recombinant Mouse sFRP-2 Protein, CF Summary

Details of Functionality
Measured by its ability to compete with Frizzled-1 for binding to biotinylated Wnt-3a. The IC50 value is 0.7-3.5 nM, under conditions in which Recombinant Mouse (rm)  Frizzled‑1 Fc Chimera (Catalog # 1120-FZ) is present at 2.1 nM, and biotinylated rmWnt-3a concentration is 2.7 nM.
Source
Mouse myeloma cell line, NS0-derived mouse sFRP-2 protein
Leu25-Cys295, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Leu25
Protein/Peptide Type
Recombinant Proteins
Gene
Sfrp2
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
31.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
35-40 kDa, reducing conditions
Publications
Read Publications using
1169-FR/CF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse sFRP-2 Protein, CF

  • FRP2
  • FRP-2secreted apoptosis related protein 1
  • SARP-1
  • SARP1sFRP-2
  • SDF-5
  • Secreted apoptosis-related protein 1
  • secreted frizzled-related protein 2
  • sFRP2
  • sFRP-2

Background

Secreted Frizzled Related Protein-2 (sFRP-2) belongs to a family of Wnt-binding proteins with homology to the ligand-binding domain of the Frizzled transmembrane Wnt receptors. The sFRP proteins are approximately 30 - 35 kDa in size and contain an N-terminal Frizzled-like domain with 10 conserved cysteines and a Netrin-like C-terminal domain (1, 2). Mature mouse sFRP-2, also known as SARP-1, SDF-5, and FRP-2, shares 99% aa sequence identity with human and rat sFRP-2 (3, 4). sFRP-2 is widely expressed during embryogenesis and in the adult in tissues including the eye, heart, lung, colon, intestine, smooth muscle, pancreas, prostate, testis, kidney, brain, teeth and joints, craniofacial mesenchyme, and preadipocytes (3 - 6). Depending on the context, sFRP-2 can exert either positive or negative effects on Wnt signaling (7 - 9). It also inhibits BMP-induced effects [Alfaro 35646, Oshima 3160]. sFRP-2 can be incorporated into the extracellular matrix through interactions with Fibronectin and Integrin alpha 5 beta 1 (11). sFRP-2 plays a variety of roles during tissue morphogenesis including inhibition of the planar cell polarity pathway and myoblast and osteoblast differentiation (8, 10, 12, 13). sFRP-2 is also expressed in multiple myeloma and glioma in which it promotes tumorigenicity (10, 14). At physiological concentrations sFRP-2 enhances BMP-1 mediated proteolysis of Pro-Collagen I, whereas at higher concentrations it inhibits BMP-1 activity (15, 16). This difference is significant considering that sFRP‑2 is up‑regulated in fibrotic areas of the heart following myocardial infarction (15, 16). Elevated levels of sFRP-2 then promote the recovery of cardiac function by reducing collagen deposition, remodeling, and calcification and by promoting the engraftment of mesenchymal stem cells into the heart (8, 15).
  1. Bovolenta, P. et al. (2008) J. Cell Sci. 121:737.
  2. van Amerongen, R. and R. Nusse (2009) Development 136:3205.
  3. Rattner, A. et al. (1997) Proc. Natl. Acad. Sci. 94:2859.
  4. Melkonyan, H.S. et al. (1997) Proc. Natl. Acad. Sci. 94:13636.
  5. Hu, E. et al. (1998) Biochem. Biophys. Res. Commun. 247:287.
  6. Leimeister, C. et al. (1998) Mech. Dev. 75:29.
  7. Galli, L.M. et al. (2006) Dev. Dyn. 235:681.
  8. Alfaro, M.P. et al. (2010) J. Biol. Chem. 285:35645.
  9. von Marschall, Z. and L.W. Fisher (2010) Biochem. Biophys. Res. Commun. 400:299.
  10. Oshima, T. et al. (2005) Blood 106:3160.
  11. Lee, J.-L. et al. (2004) J. Biol. Chem. 279:14602.
  12. Sugiyama, Y. et al. (2010) Dev. Biol. 338:193.
  13. Descamps, S. et al. (2008) Cell Tissue Res. 332:299.
  14. Roth, W. et al. (2000) Oncogene 19:4210.
  15. He, W. et al. (2010) Proc. Natl. Acad. Sci. 107:21110.
  16. Kobayashi, K. et al. (2009) Nat. Cell Biol. 11:46.

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Publications for sFRP-2 (1169-FR/CF)(7)

We have publications tested in 3 confirmed species: Mouse, Rat, Chicken.

We have publications tested in 4 applications: Bioassay, In Vivo, In Vivo grafting, Surface Plasmon Resonance.


Filter By Application
Bioassay
(3)
In Vivo
(2)
In Vivo grafting
(1)
Surface Plasmon Resonance
(1)
All Applications
Filter By Species
Mouse
(4)
Rat
(1)
Chicken
(2)
All Species
Showing Publications 1 - 7 of 7.
Publications using 1169-FR/CF Applications Species
Godoy J, Arrazola M, Ordenes D, Silva-Alvarez C, Braidy N, Inestrosa N Wnt-5a ligand modulates mitochondrial fission-fusion in rat hippocampal neurons. J Biol Chem, 2014;289(52):36179-93. 2014 [PMID: 25336659] (Bioassay, Rat) Bioassay Rat
Yamamoto-Shiraishi Y, Kuroiwa A Wnt and BMP signaling cooperate with Hox in the control of Six2 expression in limb tendon precursor., 2013;0(0):. 2013 [PMID: 23499659] (In Vivo grafting, Mouse) In Vivo grafting Mouse
Tran, Thanh H, Shi, Xiaofeng, Zaia, Joseph, Ai, Xingbin Heparan sulfate 6-O-endosulfatases (Sulfs) coordinate the Wnt signaling pathways to regulate myoblast fusion during skeletal muscle regeneration. J Biol Chem, 2012;287(39):32651-64. 2012 [PMID: 22865881] (Bioassay, Mouse) Bioassay Mouse
Wawrzak D, Metioui M, Willems E, Hendrickx M, de Genst E, Leyns L Wnt3a binds to several sFRPs in the nanomolar range. Biochem. Biophys. Res. Commun., 2007;357(4):1119-23. 2007 [PMID: 17462603] (Surface Plasmon Resonance, Mouse) Surface Plasmon Resonance Mouse
Canning CA, Lee L, Irving C, Mason I, Jones CM Sustained interactive Wnt and FGF signaling is required to maintain isthmic identity. Dev. Biol., 2007;305(1):276-86. 2007 [PMID: 17383629] (Bioassay, Chicken) Bioassay Chicken
Schmidt C, Stoeckelhuber M, McKinnell I, Putz R, Christ B, Patel K Wnt 6 regulates the epithelialisation process of the segmental plate mesoderm leading to somite formation. Dev. Biol., 2004;271(1):198-209. 2004 [PMID: 15196961] (In Vivo, Chicken) In Vivo Chicken
Polesskaya A, Seale P, Rudnicki MA Wnt signaling induces the myogenic specification of resident CD45+ adult stem cells during muscle regeneration. Cell, 2003;113(7):841-52. 2003 [PMID: 12837243] (In Vivo, Mouse) In Vivo Mouse

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Bioinformatics

Gene Symbol Sfrp2
Entrez
Uniprot