Recombinant Mouse sFRP-2 Protein, CF


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Recombinant Mouse sFRP-2 Protein, CF Summary

Details of Functionality
Measured by its ability to compete with Frizzled-1 for binding to biotinylated Wnt-3a. The IC50 value is 0.7-3.5 nM, under conditions in which Recombinant Mouse (rm)  Frizzled‑1 Fc Chimera (Catalog # 1120-FZ) is present at 2.1 nM, and biotinylated rmWnt-3a concentration is 2.7 nM.
Mouse myeloma cell line, NS0-derived mouse sFRP-2 protein
Leu25-Cys295, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Protein/Peptide Type
Recombinant Proteins
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.


  • Binding Activity
Theoretical MW
31.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
35-40 kDa, reducing conditions
Read Publications using
1169-FR/CF in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS.
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse sFRP-2 Protein, CF

  • FRP2
  • FRP-2secreted apoptosis related protein 1
  • SARP-1
  • SARP1sFRP-2
  • SDF-5
  • Secreted apoptosis-related protein 1
  • secreted frizzled-related protein 2
  • sFRP2
  • sFRP-2


Secreted Frizzled Related Protein-2 (sFRP-2) belongs to a family of Wnt-binding proteins with homology to the ligand-binding domain of the Frizzled transmembrane Wnt receptors. The sFRP proteins are approximately 30 - 35 kDa in size and contain an N-terminal Frizzled-like domain with 10 conserved cysteines and a Netrin-like C-terminal domain (1, 2). Mature mouse sFRP-2, also known as SARP-1, SDF-5, and FRP-2, shares 99% aa sequence identity with human and rat sFRP-2 (3, 4). sFRP-2 is widely expressed during embryogenesis and in the adult in tissues including the eye, heart, lung, colon, intestine, smooth muscle, pancreas, prostate, testis, kidney, brain, teeth and joints, craniofacial mesenchyme, and preadipocytes (3 - 6). Depending on the context, sFRP-2 can exert either positive or negative effects on Wnt signaling (7 - 9). It also inhibits BMP-induced effects [Alfaro 35646, Oshima 3160]. sFRP-2 can be incorporated into the extracellular matrix through interactions with Fibronectin and Integrin alpha 5 beta 1 (11). sFRP-2 plays a variety of roles during tissue morphogenesis including inhibition of the planar cell polarity pathway and myoblast and osteoblast differentiation (8, 10, 12, 13). sFRP-2 is also expressed in multiple myeloma and glioma in which it promotes tumorigenicity (10, 14). At physiological concentrations sFRP-2 enhances BMP-1 mediated proteolysis of Pro-Collagen I, whereas at higher concentrations it inhibits BMP-1 activity (15, 16). This difference is significant considering that sFRP‑2 is up‑regulated in fibrotic areas of the heart following myocardial infarction (15, 16). Elevated levels of sFRP-2 then promote the recovery of cardiac function by reducing collagen deposition, remodeling, and calcification and by promoting the engraftment of mesenchymal stem cells into the heart (8, 15).
  1. Bovolenta, P. et al. (2008) J. Cell Sci. 121:737.
  2. van Amerongen, R. and R. Nusse (2009) Development 136:3205.
  3. Rattner, A. et al. (1997) Proc. Natl. Acad. Sci. 94:2859.
  4. Melkonyan, H.S. et al. (1997) Proc. Natl. Acad. Sci. 94:13636.
  5. Hu, E. et al. (1998) Biochem. Biophys. Res. Commun. 247:287.
  6. Leimeister, C. et al. (1998) Mech. Dev. 75:29.
  7. Galli, L.M. et al. (2006) Dev. Dyn. 235:681.
  8. Alfaro, M.P. et al. (2010) J. Biol. Chem. 285:35645.
  9. von Marschall, Z. and L.W. Fisher (2010) Biochem. Biophys. Res. Commun. 400:299.
  10. Oshima, T. et al. (2005) Blood 106:3160.
  11. Lee, J.-L. et al. (2004) J. Biol. Chem. 279:14602.
  12. Sugiyama, Y. et al. (2010) Dev. Biol. 338:193.
  13. Descamps, S. et al. (2008) Cell Tissue Res. 332:299.
  14. Roth, W. et al. (2000) Oncogene 19:4210.
  15. He, W. et al. (2010) Proc. Natl. Acad. Sci. 107:21110.
  16. Kobayashi, K. et al. (2009) Nat. Cell Biol. 11:46.

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Publications for sFRP-2 (1169-FR/CF)(11)

We have publications tested in 3 confirmed species: Mouse, Rat, Chicken.

We have publications tested in 4 applications: Bioassay, In Vivo, Surface Plasmon Resonance, Western Blot Control.

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In Vivo
Surface Plasmon Resonance
Western Blot Control
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Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using 1169-FR/CF Applications Species
E Ramos-Fern, MS Arrázola, CA Oliva, SB Arredondo, L Varela-Nal, NC Inestrosa Wnt5a promotes hippocampal postsynaptic development and GluN2B-induced expression via the eIF2&amp;alpha HRI kinase Scientific Reports, 2021;11(1):7395. 2021-01-01 [PMID: 33795747] (Bioassay, Mouse) Bioassay Mouse
M Montagner, R Bhome, S Hooper, P Chakravart, X Qin, J Sufi, A Bhargava, CDH Ratcliffe, Y Naito, A Pocaterra, CJ Tape, E Sahai Crosstalk with lung epithelial cells regulates Sfrp2-mediated latency in breast cancer dissemination Nat. Cell Biol., 2020;0(0):. 2020-01-01 [PMID: 32094692] (Western Blot Control) Western Blot Control
C Chen, Y Yang, Y Yao HBO Promotes the Differentiation of Neural Stem Cells via Interactions Between the Wnt3/beta-Catenin and BMP2 Signaling Pathways Cell Transplant, 2019;0(0):9636897198835. 2019-01-01 [PMID: 31694396] (Rat) Rat
P Periasamy, V Tran, HC O'Neill Identification of genes which regulate stroma-dependent in vitro hematopoiesis PLoS ONE, 2018;13(10):e0205583. 2018-01-01 [PMID: 30308055] (Bioassay, Mouse) Bioassay Mouse
Godoy J, Arrazola M, Ordenes D, Silva-Alvarez C, Braidy N, Inestrosa N Wnt-5a ligand modulates mitochondrial fission-fusion in rat hippocampal neurons. J Biol Chem, 2014;289(52):36179-93. 2014-01-01 [PMID: 25336659] (Bioassay, Rat) Bioassay Rat
Yamamoto-Shiraishi Y, Kuroiwa A Wnt and BMP signaling cooperate with Hox in the control of Six2 expression in limb tendon precursor. , 2013;0(0):. 2013-01-01 [PMID: 23499659] (In Vivo, Mouse) In Vivo Mouse
Tran , Thanh H, Shi , Xiaofeng, Zaia , Joseph, Ai , Xingbin Heparan sulfate 6-O-endosulfatases (Sulfs) coordinate the Wnt signaling pathways to regulate myoblast fusion during skeletal muscle regeneration. J Biol Chem, 2012;287(39):32651-64. 2012-01-01 [PMID: 22865881] (Bioassay, Mouse) Bioassay Mouse
Wawrzak D, Metioui M, Willems E, Hendrickx M, de Genst E, Leyns L Wnt3a binds to several sFRPs in the nanomolar range. Biochem. Biophys. Res. Commun., 2007;357(4):1119-23. 2007-01-01 [PMID: 17462603] (Surface Plasmon Resonance, Mouse) Surface Plasmon Resonance Mouse
Canning CA, Lee L, Irving C, Mason I, Jones CM Sustained interactive Wnt and FGF signaling is required to maintain isthmic identity. Dev. Biol., 2007;305(1):276-86. 2007-01-01 [PMID: 17383629] (Bioassay, Chicken) Bioassay Chicken
Schmidt C, Stoeckelhuber M, McKinnell I, Putz R, Christ B, Patel K Wnt 6 regulates the epithelialisation process of the segmental plate mesoderm leading to somite formation. Dev. Biol., 2004;271(1):198-209. 2004-01-01 [PMID: 15196961] (In Vivo, Chicken) In Vivo Chicken
Show All 11 Publications.

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Gene Symbol Sfrp2