Recombinant Mouse Semaphorin 3C Fc Chimera (Truncated), CF

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Recombinant Mouse Semaphorin 3C Fc Chimera (Truncated), CF Summary

Details of Functionality
Measured by the ability of the immobilized protein to support the adhesion of SVEC4‑10 mouse vascular endothelial cells. When 5 x 104 cell per well are added to recombinant mouse Semaphorin 3C coated plates (10 μg/mL, 100 μL/well), approximately 60-80% will adhere after 1 hour at 37 °C.
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived mouse Semaphorin 3C protein
MHHHHHH Mouse Semaphorin 3C
(Gln24-Ser741, Arg548Ala, Arg552Ala)
Accession # Q62181
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Met
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
Sema3c
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
108.9 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
109 kDa, reducing conditions
Publications
Read Publications using
1728-S3 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Tris, Citric Acid, NaCl and Tween® 20.
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Semaphorin 3C Fc Chimera (Truncated), CF

  • (semaphorin) 3C
  • sema domain, immunoglobulin domain (Ig), short basic domain, secreted
  • Sema E
  • SEMA3C
  • SEMAE
  • Semaphorin 3C
  • Semaphorin E
  • semaphorin-3C
  • semaphorin-E
  • SEME

Background

Semaphorin 3C (Sema3C; previously semaE) is one of six Class 3 secreted semaphorins which share 40-50% amino acid (aa) identity. Class 3 semaphorins are potent chemorepellents that function in axon and/or vascular guidance during development, and may be upregulated in tumor progression (1, 2). The 751 amino acid (aa) mouse Sema3C is highly modular. It contains a 20 aa signal sequence, an ~500 aa N-terminal Sema domain that forms a beta -propeller structure similar to that found in integrin molecules, a cysteine knot, a furin-type cleavage site, an Ig-like domain, and a C-terminal basic domain (1-3). Covalent dimerization plus cleavage at the C-terminus are required for activity of class 3 semaphorins (4). Mouse Sema3C shares at least 95% aa identity with human, rat, cow and dog Sema3C, and 89% and 75% aa identity with chick and zebrafish Sema3C, respectively. Type 3 semaphorins transduce signals through transmembrane plexins, either directly or by binding associated neuropilin receptors (1, 2). Sema3C signaling is transduced by Plexin-D1 indirectly via neuropilin-1 or neuropilin-2 receptors (5). Sema3C is expressed in all somitic motor neurons, in lung buds and in cardiac neural crest cells during development (1, 5-8). Sema3C activates integrins in certain cells so, in addition to its repulsive activities, it sometimes acts as a chemoattractant (6, 9). In the developing nervous system, this chemoattraction appears to complement Sema3A repulsion in adjacent cell layers (1, 6, 7). Sema3C also provides an attractive force opposing Sema6A and Sema6B to guide migration of neural crest endothelial cells to the cardiac outflow tract (10). Consequently, defects in aortic arch formation occur when Sema3C or Plexin-D1 genes or Sema3C-neuropilin interactions are disrupted (5, 11, 12).

  1. Hinck, L. (2004) Dev. Cell 7:783.
  2. Neufeld, G. et al. (2005) Front. Biosci. 10:751.
  3. Gherardi, E. et al. (2004) Curr. Opin. Struct. Biol. 14:669.
  4. Adams, R. H. et al. (1997) EMBO J. 16:6077.
  5. Gitler, A. D. et al. (2004) Dev. Cell 7:107.
  6. Bagnard, D. et al. (1998) Development 125:5043.
  7. Cohen, S. et al. (2005) Eur. J. Neurosci. 21:1767.
  8. Puschel, A. W. et al. (1995) Neuron 14:941.
  9. Herman, J. G. and G. G. Meadows (2007) Int. J. Oncol. 30:1231.
  10. Toyofuku, T. et al. (2008) Dev. Biol. 321:251.
  11. Feiner, L. et al. (2001) Development 128:3061.
  12. Gu, C. et al. (2003) Dev. Cell 5:45.

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Publications for Semaphorin 3C (1728-S3)(2)

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Bioinformatics

Gene Symbol Sema3c
Entrez
Uniprot