Recombinant Mouse PLTP Protein

• Reactivity: Mu
• Applications: Bioactivity

Recombinant Mouse PLTP Protein Summary

• Details of Functionality: Measured by its ability to inhibit LPS-induced TNF-alpha secretion by RAW 264.7 mouse monocyte/macrophage cells. 3.3 μg/mL of rmPLTP will inhibit 65-95% of the TNF-alpha secretion induced by 0.5 ng/mL of LPS.
• Source: Mouse myeloma cell line, NS0-derived mouse PLTP protein Glu18-Ala493, with a C-terminal 6-His tag
• Accession #: AAH03782
• N-terminal Sequence: Glu18
• Protein/Peptide Type: Recombinant Proteins
• Gene: Pltp
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 53.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 70-85 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 3 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 250 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse PLTP Protein

• HDLCQ9
• Lipid transfer protein II
• phospholipid transfer protein
• PLTP

Background

Phospholipid transfer protein (PLTP; also known as lipid transfer protein II) is an 80 kDa, secreted glycoprotein, that belongs to the BPI/LBP/Plunc superfamily of molecules (1). It is synthesized as a precursor with a 17 amino acid (aa) signal sequence and a 476 aa mature chain. The mature chain contains two BPI/LBP/CETP domains (aa 21 - 239 and 263 - 463) and seven potential sites for N-linked glycosylation. Mature mouse PLTP shares 83% aa sequence identity with mature human PLTP (3). PLTP is expressed by a wide variety of tissues and is secreted by macrophages. Northern blot analysis shows the order of PLTP mRNA expression as follows: lung > adipose tissue, placenta, testis > brain > muscle, heart, liver (2). PLTP is a multifunctional, extracellular lipid transfer protein that plays a major role in lipoprotein metabolism and atherosclerosis (4 - 6). In addition to phospholipids, PLTP transfers several amphipatic compounds, including unesterified cholesterol, lipopolysaccharides, diacylglycerides, and alpha -tocopherol, the main isomer of vitamin E (4). According to several studies, PLTP not only affects plasma lipid and lipoprotein profiles, but also modulates cell physiology (4, 7 - 11). In mice, systemic PLTP deficiency proves to be atheroprotective, whereas PLTP overexpression is proatherogenic (4, 6). However, macrophage PLTP is shown to be atheroprotective (4, 6).

1. Jauhiainen, M. and C. Ehnholm (2005) Methods 36:97.
2. Jiang, X.-C. and C. Bruce (1995) J. Biol. Chem. 270:17133.
3. Albers, J.J. et al. (1995) Biochim. Biophys. Acta. 1258:27.
4. Ogier, N. et al. (2007) Arterioscler. Thromb. Vasc. Biol. 27:2407.
5. Huuskonen, J. and C. Ehnholm (2000) Curr. Opin. Lipidol. 11:285.
6. Valenta, D.T. et al. (2008) J. Lipid Res. 49:24.
7. Jiang, X.-C. et al. (1998) J. Biol. Chem. 273:15714.
8. Jiang, X.-C. et al. (2005) J. Biol. Chem. 280:18336.
9. Desrumaux, C. et al. (2005) FASEB J. 19:296.
10. Drouineaud, V. et al. (2006) FASEB J. 20:794.
11. Klein, A. et al. (2006) Arterioscler. Thromb. Vasc. Biol. 26:2160.

Publications for PLTP (4918-PL)(1)

Publication using 4918-PL

• Brehm A, Geraghty P, Campos M, Garcia-Arcos I, Dabo A, Gaffney A, Eden E, Jiang X, D'Armiento J, Foronjy R Cathepsin G degradation of phospholipid transfer protein (PLTP) augments pulmonary inflammation. FASEB J, 2014-02-14;28(5):2318-31. 2014-02-14 [PMID: 24532668] (Bioassay, Human)

Bioinformatics

• Gene Symbol: Pltp
• Uniprot:
  • AAH03782()