Recombinant Mouse Plexin C1 Protein, CF

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse Plexin C1 Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. When recombinant mouse Plexin C1 is coated at 5 μg/mL (100 μL/well), recombinant mouse Semaphorin 7A/Fc Chimera (Catalog # 1835-S3) binds with an apparent K_D <5 nM.
• Source: Chinese Hamster Ovary cell line, CHO-derived mouse Plexin C1 protein
  Ala35-Thr950, with a C-terminal 6-His tag
• Accession #: Q9QZC2
• N-terminal Sequence: Ala35
• Protein/Peptide Type: Recombinant Proteins
• Gene: Plxnc1
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 101.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 130-145 kDa, under reducing conditions.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Plexin C1 Protein, CF

• CD232 antigen
• CD232
• plexin (semaphorin receptor)
• Plexin C1
• PLXNC1
• PLXN-C1
• receptor for viral semaphorin protein
• receptor for virally-encoded semaphorin
• VESPR
• VESPRplexin-C1
• Virus-encoded semaphorin protein receptor

Background

Plexin C1, also known as Virus-encoded semaphorin protein receptor (VESPR) and CD232, is a 210 kDa type I transmembrane glycoprotein in the C subfamily of the plexin family (1, 2). Human Plexin C1 contains a 34 amino acid (aa) signal sequence, a 910 aa extracellular domain (ECD) with one Sema-domain and two cysteine-rich Met-related sequences (MRS), a 21 aa transmembrane domain and a 603 aa cytoplasmic domain that includes features common to other plexins, including a phosphothreonine site. The mouse Plexin C1 ECD shares 85%, 78% and 65% aa identity with human, bovine and equine Plexin C1, respectively. A truncated rat Plexin C1 cDNA that encodes an apparently soluble form shares 93% aa identity with mouse Plexin C1 within aa 263 ‑ 932 (3). Plexin C1 is widely expressed in neuronal and non‑neuronal fetal and adult tissues (4). In neuronal development, its role is unclear. Semaphorin-7a (Sema7a, CD108) binds Plexin C1 in vitro and the two show a similar expression pattern during rat neuronal development, but beta 1 integrins rather than Plexin C1 appear to mediate Sema7a effects on axon outgrowth (5, 6). Plexin C1 does appear to play a role in partitioning of paraventricular and supraoptic neurons in the hypothalamus, as indicated by specific defects seen in mice deleted for Plexin C1 (7). In the immune system, effects of Sema7a on T cell‑mediated inflammatory responses also appear to be mediated by beta 1 integrins rather than plexins (8). However, Plexin C1 may function to oppose the effect of beta 1 integrins, as it does on Sema7a-mediated spreading and dendrite formation in melanocytes (9). Its expression is lost in melanoma metastasis, suggesting a role as a tumor suppressor (10). Plexin C1 is the receptor for poxvirus (A39R protein) and herpes virus (AHVsema) semaphorin homologs, and mediates activation of monocytes and inhibition of dendritic cell and neutrophil phagocytosis and migration by A39R (2, 11, 12).

1. Negishi, M. et al. (2005) Cell. Mol. Life Sci. 62:1363.
2. Comeau, M.R. et al. (1998) Immunity 8:473.
3. NCBI Reference Sequence: NP_001102214.
4. Perala, N.M. et al. (2005) Gene Expr. Patterns 5:355.
5. Pasterkamp, R.J. et al. (2007) BMC Dev. Biol. 7:98.
6. Pasterkamp, R.J. et al. (2003) Nature 424:398.
7. Xu, C. & C-M. Fan (2007) Mol. Endocrinol. 21:1234.
8. Suzuki, K. et al. (2007) Nature 446:680.
9. Scott, G.A. et al. (2007) J. Invest. Dermatol. 128:151.
10. Lazova, R. et al. (2009) Am. J. Dermatopathol. 31:177.
11. Walzer, T. et al. (2005) J. Immunol. 174:51.
12. Walzer, T. et al. (2005) Eur. J. Immunol. 35:391.

Publications for Plexin C1 (5375-PC)(1)

Publication using 5375-PC

• Parkash J, Messina A, Langlet F, Cimino I, Loyens A, Mazur D, Gallet S, Balland E, Malone S, Pralong F, Cagnoni G, Schellino R, De Marchis S, Mazzone M, Pasterkamp R, Tamagnone L, Prevot V, Giacobini P Semaphorin7A regulates neuroglial plasticity in the adult hypothalamic median eminence. Nat Commun, 2015-02-27;6(0):6385. 2015-02-27 [PMID: 25721933] (In Vivo, Rat)

Bioinformatics

• Gene Symbol: Plxnc1
• Uniprot:
  • Q9QZC2()