Recombinant Mouse Plexin A2 Protein, CF

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse Plexin A2 Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. When Recombinant Mouse Plexin A2 is coated at 3 μg/mL, Recombinant Human Semaphorin 6A Fc Chimera (Catalog # 1146-S6) binds with an apparent K_D <25 nM.
• Source: Chinese Hamster Ovary cell line, CHO-derived mouse Plexin A2 protein
  Met25-Pro1227, with a C-terminal 6-His tag
• Accession #: BAA13189
• N-terminal Sequence: Met25
• Protein/Peptide Type: Recombinant Proteins
• Gene: Plxna2
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 133 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 140-160 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 400 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Plexin A2 Protein, CF

• FLJ11751
• FLJ30634
• KIAA0463plexin 2
• OCT
• OCTSemaphorin receptor OCT
• Plexin A2
• PLXN2
• PLXN2plexin-A2
• PLXNA2
• transmembrane protein OCT

Background

Plexin A2, originally called OCT in human or Plexin 2 in mouse, is a 220-230 kDa type I transmembrane glycoprotein of the Plexin A subfamily of Plexin proteins (1-3). Mouse Plexin A2 encodes 1894 amino acids (aa) including a 34 aa signal peptide, a 1203 aa extracellular domain (ECD), a 21 aa transmembrane sequence, and a 636 aa cytoplasmic region. The ECD contains one Sema domain (aa 35-508) and four IPT regions (aa 858-1228). Within the ECD, mouse Plexin A2 shares aa sequence identity of 96% with human, equine, canine and bovine Plexin A2, and 99% with rat Plexin A2. The four mouse Plexin A proteins share 59-66% aa sequence identity with each other and show distinct, but sometimes overlapping, expression and interactions (4, 5). Plexin A2 participates in cell migration and axon guidance by serving as a receptor for transmembrane Semaphorins 6A and 6B, and as a coreceptor with Neuropilin-1 for secreted Semaphorins 3A and 3C (6-13). In the developing mouse, Plexin A2 is found during nervous system development on hippocampal CA3 pyramidal cells and dentate granule cells, cerebellar granule cells, olfactory neurons, sensory neurons and spinal motor neurons (4-10). During cardiac morphogenesis, it is found on cardiac neural crest cells and is important in cardiac outflow tract formation (10). Expression is also reported in the mouse embryo oral epithelium, lung mesenchyme, and intestinal muscularis externa (4). Plexin A2 is induced by BMP2 in differentiating osteoblasts and promotes their maturation (14).

1. Perala, N. et al. (2012) Differentiation 83:77.
2. Kameyama, T. et al. (1996) Biochem. Biophys. Res. Commun. 226:524.
3. Maestrini E. et al. (1996) Proc. Natl. Acad. Sci. USA 93:674.
4. Perala, N.M. et al. (2005) Gene Expr. Patterns 5:355.
5. Murakami, Y. et al. (2001) Dev. Dyn. 220:246.
6. Suto, F. et al. (2007) Neuron 53:535.
7. Tawarayama, H. et al. (2010) J. Neurosci. 30:7049.
8. Renaud, J. et al. (2008) Nat. Neurosci. 11:440.
9. Bron, R. et al. (2007) Neural Dev. 2:21.
10. Toyofuku, T. et al. (2008) Dev. Biol. 321:251.
11. Nogi, T. et al. (2010) Nature 467:1123.
12. Janssen, B.J.C. et al. (2010) Nature 467:1118.
13. Rohm, B. et al. (2000) Mech. Dev. 93:95.
14. Oh, J.E. et al. (2011) J. Bone Miner Res. Nov. 16 [Epub ahead of print].

Bioinformatics

• Gene Symbol: Plxna2
• Uniprot:
  • BAA13189()