Recombinant Mouse OX40 Ligand/TNFSF4 Protein, CF

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse OX40 Ligand/TNFSF4 Protein, CF Summary

• Details of Functionality: Measured by its ability to co-stimulate IL-2 secretion by mouse T cells in the presence of anti-CD3. The ED₅₀ for this effect is 10-30 ng/mL in the presence of a cross-linking antibody, Mouse Anti-polyHistidine Monoclonal Antibody (Catalog # MAB050).
• Source: Mouse myeloma cell line, NS0-derived mouse OX40 Ligand/TNFSF4 protein
  Gln49-Leu198, with an N-terminal 10-His tag
• Accession #: P43488
• N-terminal Sequence: His
• Protein/Peptide Type: Recombinant Proteins
• Gene: Tnfsf4
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 18 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 18-28 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse OX40 Ligand/TNFSF4 Protein, CF

• CD134 ligand
• CD134L
• CD252 antigen
• CD252
• Glycoprotein Gp34
• gp34
• OX40 antigen ligand
• OX40 Ligand
• OX40L
• OX-40L
• OX4OL
• TAX transcriptionally-activated glycoprotein 1
• tax-transcriptionally activated glycoprotein 1 (34kD)
• tax-transcriptionally activated glycoprotein 1, 34kD
• TNFSF4
• tumor necrosis factor (ligand) superfamily, member 4
• tumor necrosis factor ligand superfamily member 4
• TXGP1

Background

OX40 Ligand (OX40L), also known as gp34, is a type II transmembrane glycoprotein belonging to the TNF superfamily. Murine OX40L cDNA encodes a 198 amino acid (aa) residue protein comprised of a 28 aa N-terminal cytoplasmic domain, a 20 aa transmembrane segment, and a 150 aa C-terminal extracellular domain (1). Human and murine OX40L share 46% sequence identity at the amino acid level (1). The OX40L is expressed on activated antigen presenting cells such as B cells, macrophages, dendritic cells, and on endothelial cells at the site of inflammation. The receptor for OX40L is OX40 (CD134) that is expressed predominantly on activated CD4⁺ T cells. Expression of OX40 is transient following engagement of T cell receptors (2). Ligation of OX40L by OX40 stimulates proliferation and differentiation of activated B cells, and increases immunoglobulin secretion (3, 4). The expression of OX40L on B cells is up-regulated by CD40 ligation (3). Engagement of the OX40-OX40L system has co-stimulatory effects on T cells by stimulating the production of cytokines by T helper cells and increasing the survival of memory T cells (2, 5). Blocking of the OX40-OX40L interaction in vitro inhibits co-stimulation resulting in decreased T cell proliferation and adhesion of T cells to endothelial cells. Inhibition of the OX40-OX40L interaction in disease models has beneficial effects in acute graft-versus-host disease, inflammatory bowel disease and decreases the development of collagen-induced arthritis and experimental leishmaniasis (6).

1. Baum, P.R. et al. (1994) EMBO J. 13:3992.
2. Gramaglia, I. et al. (1999) J. Immunol. 161:6510.
3. Stuber, E. et al. (1995) Immunity 2:507.
4. Malstrom, V. et al. (2001) J. Immunol. 166:6972.
5. Maxwell, J.R. et al. (2000) J. Immunol. 164:107.
6. Weinberg, A.D. (2002) Trends Immunol. 23:102.

Publications for OX40 Ligand/TNFSF4 (1236-OX/CF)(7)

Publications using 1236-OX/CF

• P Zhang, GH Tu, J Wei, P Santiago, LR Larrabee, S Liao-Chan, T Mistry, ML Chu, T Sai, K Lindquist, H Long, J Chaparro-R, S Salek-Arda, YA Yeung Ligand-Blocking and Membrane-Proximal Domain Targeting Anti-OX40 Antibodies Mediate Potent T Cell-Stimulatory and Anti-Tumor Activity Cell Rep, 2019-06-11;27(11):3117-3123.e5. 2019-06-11 [PMID: 31189099] (Bioassay, Human)
• G Sun, H Jin, C Zhang, H Meng, X Zhao, D Wei, X Ou, Q Wang, S Li, T Wang, X Sun, W Shi, D Tian, K Liu, H Xu, Y Tian, X Li, W Guo, J Jia, Z Zhang, D Zhang OX40 Regulates Both Innate and Adaptive Immunity and Promotes Nonalcoholic Steatohepatitis Cell Rep, 2018-12-26;25(13):3786-3799.e4. 2018-12-26 [PMID: 30590049] (Bioassay, Mouse)
• T Sekiya, S Hibino, K Saeki, M Kanamori, S Takaki, A Yoshimura Nr4a Receptors Regulate Development and Death of Labile Treg Precursors to Prevent Generation of Pathogenic Self-Reactive Cells Cell Rep, 2018-08-07;24(6):1627-1638.e6. 2018-08-07 [PMID: 30089271] (Bioassay, Mouse)
• W Lei, D Zeng, G Liu, Y Zhu, J Wang, H Wu, J Jiang, J Huang Crucial role of OX40/OX40L signaling in a murine model of asthma Mol Med Rep, 2018-01-18;0(0):. 2018-01-18 [PMID: 29344664] (In Vivo, Mouse)
• X Xiao, Y Fan, J Li, X Zhang, X Lou, Y Dou, X Shi, P Lan, Y Xiao, L Minze, XC Li Guidance of super-enhancers in regulation of IL-9 induction and airway inflammation J. Exp. Med., 2018-01-16;0(0):. 2018-01-16 [PMID: 29339447] (Cell Culture, Mouse)
• Arduin E, Arora S, Bamert P, Kuiper T, Popp S, Geisse S, Grau R, Calzascia T, Zenke G, Kovarik J Highly reduced binding to high and low affinity mouse Fc gamma receptors by L234A/L235A and N297A Fc mutations engineered into mouse IgG2a. Mol Immunol, 2014-10-18;63(2):456-63. 2014-10-18 [PMID: 25451975] (Binding Assay, Mouse)
• Kayamuro H, Yoshioka Y, Abe Y, Katayama K, Yoshida T, Yamashita K, Yoshikawa T, Hiroi T, Itoh N, Kawai Y, Mayumi T, Kamada H, Tsunoda S, Tsutsumi Y TNF superfamily member, TL1A, is a potential mucosal vaccine adjuvant. Biochem. Biophys. Res. Commun., 2009-05-04;384(3):296-300. 2009-05-04 [PMID: 19406102] (In Vivo, Mouse)

Bioinformatics

• Gene Symbol: Tnfsf4
• Uniprot:
  • P43488()