Recombinant Mouse Neuroplastin Protein, CF

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse Neuroplastin Protein, CF Summary

• Details of Functionality: Measured by its ability to enhance neurite outgrowth of E18-E20 rat embryonic cortical neurons. Recombinant Mouse Neuroplastin immobilized at 5-10 μg/mL is able to significantly induce neurite outgrowth.
• Source: Mouse myeloma cell line, NS0-derived mouse Neuroplastin protein
  Gln29-Pro338, with a C-terminal 6-His tag
• Accession #: P97300
• N-terminal Sequence: No results obtained: Gln29 predicted
• Protein/Peptide Type: Recombinant Proteins
• Gene: Nptn
• Purity: >95%, by SDS-PAGE with silver staining
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 36 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 40-65 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE with silver staining
• Reconstitution Instructions: Reconstitute at 250 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Neuroplastin Protein, CF

• AW554172
• GP55
• GP55Stromal cell-derived receptor 1
• GP65SDR-1
• GP66
• MGC102805
• Neuroplastin
• np55
• np65
• NPTN
• SDFR1
• SDFR1DKFZp686L2477
• SDR1
• SDR1stromal cell derived factor receptor 1

Background

Neuroplastin (Np65), also known as gp65 and SDR1, is a widely expressed and variably glycosylated transmembrane member of the CD147/Basigin protein family (1, 2). The 62-75 kDa mature mouse Neuroplastin consists of a 310 amino acid (aa) extracellular region (ECD) with three immunoglobulin-like domains, a 21 aa transmembrane segment, and a 38 aa cytoplasmic domain (3, 4). Within the ECD, mouse Neuroplastin shares 94% and 98% aa sequence identity with human and rat Neuroplastin, respectively. Alternative splicing of mouse Neuroplastin generates additional isoforms that lack the first Ig-like domain, most of the first two Ig-like domains, and/or carry a 9 aa substitution in the cytoplasmic domain. The isoform lacking the first Ig-like domain is known as Neuroplastin-55. Neuroplastin is expressed on cerebellar Purkinje cells and granule cells, hippocampal pyramidal neurons, and retinal photoreceptors (5 - 9). It serves as a homophilic cell adhesion molecule on neurons and promotes neurite outgrowth (7, 8). The neurite outgrowth function requires its binding to and activation of FGFR1 (7, 10). In the synapse, Neuroplastin binds to multiple subunits of the GABA-A receptor, reduces synaptic expression of GluR1, and contributes to synaptic plasticity (7, 8, 11, 12). Neuroplastin overexpression can enhance tumor growth, VEGF production, and vascularization (13).

1. Owczarek, S. and V. Berezin (2012) Int. J. Biochem. Cell Biol. 44:1.
2. Langnaese, K. et al. (1998) FEBS Lett. 429:284.
3. Shirozu, M. et al. (1996) Genomics 37:273.
4. Langnaese, K. et al. (1997) J. Biol. Chem. 272:821.
5. Marzban, H. et al. (2003) J. Comp. Neurol. 462:286.
6. Bernstein, H-G. et al. (2007) Brain Res. 1134:107.
7. Owczarek, S. et al. (2011) J. Neurochem. 117:984.
8. Smalla, K-H. et al. (2000) Proc. Natl. Acad. Sci. USA 97:4327.
9. Kreutz, M.R. et al. (2001) Invest. Ophthalmol. Vis. Sci. 42:1907.
10. Owczarek, S. et al. (2010) FASEB J. 24:1139.
11. Sarto-Jackson, I. et al. (2012) J. Biol. Chem. 287:14201.
12. Empson, R.M. et al. (2006) J. Neurochem. 99:850.
13. Rodriguez-Pinto, D. et al. (2009) Cancer Immunol. Immunother. 58:221.

Bioinformatics

• Gene Symbol: Nptn
• Uniprot:
  • P97300(Mouse)