Recombinant Mouse Neogenin Fc Chimera Protein, CF

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications Binding Activity
Format
Carrier-Free

Order Details

Recombinant Mouse Neogenin Fc Chimera Protein, CF Summary

Details of Functionality
Measured by its binding ability in a functional ELISA. Immobilized Recombinant Mouse Neogenin Fc Chimera at 5 µg/mL (100 µL/well) can bind Recombinant Chicken Netrin‑1 (Catalog # 128-N1) with a linear range of 6-400 ng/mL.
Source
Mouse myeloma cell line, NS0-derived mouse Neogenin protein
Mouse Neogenin
(Ala42-Ile1033)
(Asp442-Leu461 del)
Accession # NP_032710
IEGRID Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Ala42
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
Neo1
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
133 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
155-175 kDa, reducing conditions
Publications
Read Publication using
1079-NE in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Neogenin Fc Chimera Protein, CF

  • HsT17534
  • IGDCC2DKFZp547B146
  • Immunoglobulin superfamily DCC subclass member 2
  • NEO
  • NEO1
  • neogenin (chicken) homolog 1
  • neogenin 1
  • neogenin homolog 1
  • Neogenin
  • NGN
  • NGNDKFZp547A066

Background

Neogenin is a type I transmembrane protein belonging to the Ig superfamily. It is composed of an extracellular segment containing four Ig-like C2-type domains and six Fibronectin type III domains (1). Neogenin has a molecular weight of approximately 190 kDa, and the extracellular domain of the mouse protein shares 91% and 94% amino acid sequence identity with the human and rat orthologs, respectively (1). Five different isoforms are produced from alternative splicing of mouse Neo1. Neogenin is widely expressed in both neuronal and non-neuronal tissues of the developing mouse embryo, and in most tissues in adult mice (2). It is a multifunctional cell-surface receptor that binds to members of the Netrin, Repulsive Guidance Molecule (RGM) and Bone Morphogenetic Protein (BMP) families (3-5). It has also been shown to interact with members of the UNC5 family and in certain instances, associate with CDO as a co-receptor (6-8). Neogenin appears to be involved in the regulation of multiple developmental processes including development of the central nervous system (CNS), myogenesis, angiogenesis, and formation of mammary glands (4, 5, 7-9). During CNS development, Neogenin regulates neural tube closure, neuronal differentiation, and cell survival (4, 5, 7). It also mediates
Netrin-1-dependent attraction and RGM-A-dependent repulsion of growing axons (4, 5, 7, 10). Additionally, Neogenin binding to RGM and Netrin proteins regulates cell-cell adhesion, cell migration, tissue organization, and adult neurogenesis (4, 7, 11). Neogenin is thought to be involved in tumorgenesis and cancer cell invasiveness in brain and gastric cancers (12-14).
  1. Meyerhardt, J.A. et al. (1997) Oncogene 14:1129.
  2. Keeling, S.L. et al. (1997) Oncogene 15:691.
  3. Hagihara, M. et al. (2011) J. Biol. Chem. 286:5157.
  4. Tian, C. and J. Liu (2013) Mol. Reprod. Dev. 80:700.
  5. Severyn, C.J. et al. (2009) Biochem. J. 422:393.
  6. van den Heuvel, D.M. et al. (2013) PLoS ONE 8:e55828.
  7. De Vries, M. and H.M. Cooper (2008) J. Neurochem. 106:1483.
  8. Krauss, R.S. et al. (2005) J. Cell Sci. 118:2355.
  9. Srinivasan, K. et al. (2003) Dev. Cell 4:371.
  10. de Castro, F. (2003) News Physiol. Sci. 18:130.
  11. O’Leary, C.J. et al. (2015) Stem Cells 33:503.
  12. Milla, L.A. et al. (2014) Int. J. Cancer 134:21.
  13. Akino, T. et al. (2014) Cancer Res. 74:3716.
  14. Kim, S.J. et al. (2014) Oncotarget 5:3386.

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Publications for Neogenin (1079-NE)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: ELISA capture.


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(1)
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Bioinformatics

Gene Symbol Neo1
Uniprot