Recombinant Mouse NELL1 Protein, CF

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse NELL1 Protein, CF Summary

• Details of Functionality: Measured by the ability of the immobilized protein to support the adhesion of C3H10T1/2 mouse embryonic fibroblast cells. The ED₅₀ for this effect is 1-4 μg/mL.
• Source: Chinese Hamster Ovary cell line, CHO-derived mouse NELL1 protein
  Arg17-Asn810, with a C-terminal 6-His tag
• Accession #: Q2VWQ2
• N-terminal Sequence: Arg17 & Phe22
• Protein/Peptide Type: Recombinant Proteins
• Gene: Nell1
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 88.4 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 125-165 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 200 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse NELL1 Protein, CF

• FLJ45906
• IDH3GL
• nel (chicken)-like 1
• NELL1
• NEL-like 1 (chicken)
• NEL-like protein 1
• Nel-related protein 1
• neural epidermal growth factor-like 1
• NRP1
• protein kinase C-binding protein NELL1

Background

NELL1 (neural EGF‑like like protein 1) is an approximately 140 kDa modular glycoprotein that  plays an important role in bone physiology (1). NELL1 contains an N‑terminal Laminin G‑like domain and three vWF‑C domains interspersed with five tandem EGF‑like domains (2). Mature mouse NELL1 shares 98% and 93% aa identity with rat and human NELL1, respectively. NELL1 can be retained in the cytosol where it interacts with ARP3 (apoptosis‑related protein 3) and becomes phosphorylated by PKC (3, 4). NELL1 is secreted as an approximately 400 kDa noncovalent homotrimer of heavily glycosylated subunits (5). It is expressed in bone and in select B cell lines (6, 7). NELL1 promotes the osteogenic differentiation of adipose‑derived stromal/stem cells and inhibits adipogenic differentiation (8). It does not promote osteoblastic differentiation from myoblasts, but it does enhance the activity of BMP‑2 in that function (9). NELL1 interacts directly with osteoblasts via Integrin alpha 3 beta 1 (10, 11) and promotes osteoblast differentiation and mineralization (3, 12, 13). It synergizes with BMP‑2 to increase phosphate uptake through the transporters Pit1 and Pit2 in pre‑osteoblasts (14). In vivo, NELL1 expression in the cranium is localized at sites of suture closure (6). Its overexpression induces multiple abnormalities in cranial development including premature suture closure (craniosyntosis) and overlapping sutures (12, 13). Experimental engrafting demonstrates the ability of NELL1 to promote new bone formation and the healing of calvarial defects (13, 15).

1. Zhang, X. et al. (2010) J. Dent. Res. 89:865.
2. Desai, J. et al. (2006) Hum. Mol. Genet. 15:1329.
3. Zou, X. et al. (2011) FEBS Lett. 585:2410.
4. Kuroda, S. and K. Tanizawa (1999) Biochem. Biophys. Res. Commun. 265:752.
5. Kuroda, S. et al. (1999) Biochem. Biophys. Res. Commun. 265:79.
6. Ting, K. et al. (1999) J. Bone Miner. Res. 14:80.
7. Luce, M.J. and P.D. Burrows (1999) Gene 231:121.
8. James, A.W. et al. (2012) Stem Cells Dev. 21:2170.
9. Cowan, C.M. et al. (2007) J. Bone Miner. Res. 22:918.
10. Hasebe, A. et al. (2012) FEBS Lett. 586:2500.
11. Shen, J. et al. (2012) J. Cell. Biochem. 113:3620.
12. Zhang, X. et al. (2002) J. Clin. Invest. 110:861.
13. Aghaloo, T. et al. (2006) Am. J. Pathol. 169:903.
14. Cowan, C.M. et al. (2012) Biochem. Biophys. Res. Commun. 422:351.
15. Xue, J. et al. (2011) Bone 48:485.

Publications for NELL1 (7109-NL)(1)

Publication using 7109-NL

• Alison C. McGarvey, Stanislav Rybtsov, Céline Souilhol, Sara Tamagno, Ritva Rice, David Hills et al. A molecular roadmap of the AGM region reveals BMPER as a novel regulator of HSC maturation Journal of Experimental Medicine 2017-12-04 [PMID: 29093060] (Bioassay, Mouse)

Bioinformatics

• Gene Symbol: Nell1
• Uniprot:
  • Q2VWQ2(Human)