Recombinant Mouse Mrc2 His-tag Protein, CF

When Recombinant Mouse Mrc2 His-tag (10491-EN) is immobilized at 1 µg/mL (100 µL/well), Recombinant Human Pro-Collagen I alpha 1 (6220-CL) binds with an ED50 of 0.03-0.36 μg/mL.

2 μg/lane of Recombinant Mouse Mrc2 His-tag (10491-EN) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® blue staining, showing bands at 145-165 kDa.

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse Mrc2 His-tag Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. When Recombinant Mouse Mrc2 His-tag (Catalog # 10491-EN) is immobilized at 1 µg/mL (100 µL/well), Recombinant Human Pro-Collagen I alpha 1 (Catalog # 6220-CL) binds with an ED₅₀ of 0.03-0.36 μg/mL.
• Source: Mouse myeloma cell line, NS0-derived mouse Mrc2 protein
  Ala31-Ala1413, with a C-terminal 6-His tag
• Accession #: AAI16643.1
• N-terminal Sequence: Ala31
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 157 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 130-150 kDa, under reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Mrc2 His-tag Protein, CF

• CD280 antigen
• CD280
• CD280C-type mannose receptor 2
• CLEC13E
• CLEC13EUPAR-associated protein
• Endo180
• ENDO180C-type lectin domain family 13 member E
• Endocytic receptor 180
• KIAA0709FLJ35911
• Macrophage mannose receptor 2
• mannose receptor, C type 2
• Mrc2
• uPARAP
• UPARAPendocytic receptor (macrophage mannose receptor family)
• urokinase plasminogen activator receptor-associated protein
• Urokinase receptor-associated protein
• Urokinase-type plasminogen activator receptor-associated protein

Background

Mrc2 (C-type Mannose Receptor 2), also known as MMR2, Endocytic Receptor 180 and uPARAP,  is a 180‑kDa type I transmembrane protein. It is one of the mannose receptor (MR) family members which share a common domain organization and have a broad range of biological functions (1). Mrc2 is an endocytic receptor that is found on migrating cells, including cancer cells, macrophages, fibroblasts and endothelial cells (2). Mature mouse Mrc2 is composed of 1449 amino acid (aa) that includes a 1383 aa extracellular domain (ECD), a 21 aa transmembrane region, and a 45 aa cytoplasmic domain. The ECD shows one ricin B‑type lectin domain, one fibronectin type II domain and eight C‑type lectin domains. Within the ECD, mouse Mrc2 shares 91% and 97% aa identity with human and rat Mrc2, respectively.  Mrc2 plays an important role in extracellular matrix remodeling through interaction with its ligands, including Man, Fuc, NAcGlc, collagens and urokinase plasminogen activator receptor (uPAR) (1-3). This cell surface molecule has been reported to promote cell invasion through matrix remodeling by internalizing large fragments of collagen and routing it to the lysosome for intracellular degradation and cell chemotaxis (2). It has also been reported to interact with matrix metalloprotease-13 (MMP-13) and collagen V on the cell surface (4).

1. Yuan, C. et al. (2016) Biochem. J. 473:2359.
2. Durrel, T. et al. (2018) Nat. Commun. 9:5178.
3. Behrendt, N. et al. (2000) J. Biol. Chem. 275:1993.
4. Englehom, L.H. et al. (2001) Lab. Invest.. 81:1403.

Bioinformatics

• Uniprot:
  • AAI16643.1()