Recombinant Mouse MESDC2 Protein, CF

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse MESDC2 Protein, CF Summary

• Details of Functionality: Measured by its ability to inhibit Wnt-3a-induced alkaline phosphatase production by MC3T3‑E1 mouse preosteoblast cells. The typical ND₅₀ for this effect is 2-8 µg/mL.
• Source: E. coli-derived mouse MESDC2 protein
  Ala30-Leu224
• Accession #: Q9ERE7.1
• N-terminal Sequence: Ala30
• Protein/Peptide Type: Recombinant Proteins
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 22 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse MESDC2 Protein, CF

• BOCA
• KIAA0081Renal carcinoma antigen NY-REN-61
• MESDC2
• MESDLDLR chaperone MESD
• mesoderm development candidate 2Mesoderm development protein

Background

Mesoderm development candidate gene 2 (MESDC2, Mesd), also known as Boca in drosophila, is a 22 kDa protein that is required for formation of the primitive streak and mesoderm during embryogenesis (1 - 3). Mature mouse MESDC2 consists of an 83 amino acid (aa) structured central domain with flexible N- and C-terminal regions (4, 5). It shares 89% and 96% aa sequence identity with human and rat MESDC2, respectively. Although Boca lacks 34 aa in the C-terminal region and is only 40% identical with mouse MESDC2, the mouse protein can functionally substitute for Boca in drosophila S2 cells (6). A C-terminal ER retention motif localizes MESDC2 and Boca to the lumen of the endoplasmic reticulum (2, 6, 7). Within the ER, MESDC2 binds to the Wnt co-receptors LRP5 and LRP6 and is required for their proper folding and cell surface expression (2, 5, 8, 9). MESDC2 is therefore important for cellular Wnt responsiveness (9). When added extracellularly, MESDC2 binds to cell surface LRP6, preventing its interaction with the Wnt antagonist Dkk-1. This binding does not, however, trigger LRP6 internalization or alteration of cytoplasmic beta -catenin levels (8). An LRP5 mutant associated with high bone mass does not interact with MESDC2 (10). MESDC2 itself can be disrupted by a chromosomal translocation occurring in the germ cell tumor, infantile sacrococcygeal teratoma (11).

1. Holdener, B.C. et al. (1994) Development 120:1335.
2. Hsieh, J.-C. et al. (2003) Cell 112:355.
3. Kimelman, D. (2006) Nat. Rev. Genet. 7:360.
4. Wines, M.E. et al. (2001) Genomics 72:88.
5. Koduri, V. and S.C. Blacklow (2007) Biochemistry 46:6570.
6. Culi, J. et al. (2004) EMBO J. 23:1372.
7. Culi, J. and R.S. Mann (2003) Cell 112:343.
8. Li, Y. et al. (2005) J. Cell Sci. 118:5305.
9. Li, Y. et al. (2006) FEBS Lett. 580:5423.
10. Zhang, Y. et al. (2004) Mol. Cell. Biol. 24:4677.
11. Veltman, I.M. et al. (2005) Hum. Mol. Genet. 14:1955.

Bioinformatics

• Uniprot:
  • Q9ERE7.1()