Recombinant Mouse MDGA1 Protein, CF

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse MDGA1 Protein, CF Summary

• Details of Functionality: Measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons. Able to significantly enhance neurite outgrowth when immobilized at 1.9-15 μg/mL on a nitrocellulose-coated microplate.
• Source: Mouse myeloma cell line, NS0-derived mouse MDGA1 protein
  Gln19-Pro910, with a C-terminal 6-His tag
• Accession #: NP_001074629
• N-terminal Sequence: No results obtained: Gln19 predicted, sequencing might be blocked
• Structure / Form: Noncovalently-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: Mdga1
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 99.8 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 115-125 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 300 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse MDGA1 Protein, CF

• FLJ45018
• GPIM
• MAM domain containing glycosylphosphatidylinositol anchor 1
• MAMDC3
• MDGA1

Background

MDGA1 (MAM domain containing glycosylphosphatidylinositol anchor 1) is a 135-140 kDa glycoprotein within the IgCAM superfamily of adhesion molecules (1-3). MDGA1 is thought to mediate neuronal migration, neurite outgrowth, and cell-cell adhesion within the central and peripheral nervous system (1, 4-8). Mouse MDGA1 precursor is a 940 amino acid (aa) protein that produces an 899 aa mature protein with six Ig-like domains, a fibronectin type III domain (aa 622-724), a MAM domain (aa 736-903), and a GPI anchor (aa 917) (1-3). Mature mouse MDGA1 shares 95%, 97%, and 94% aa sequence identity with mature human, rat, and canine MDGA1, respectively. Mouse MDGA1 also shares 52% aa sequence identity with mouse MDGA2, and may also share some functional redundancy (1, 4). MDGA1 is mainly expressed on restricted populations of neurons in the central and peripheral nervous system, such as embryonic neurons destined for cortical layers 2/3, migrating basilar pontine neurons and D1 interneurons of the spinal cord (1, 5-7). Deletion or down‑regulation of mouse MDGA1 slows radial migration of neurons, indicating a role for MDGA1 in radial migration of cortical neurons (4, 5). MAM and Ig-like domains are involved in heterophilic and homophilic adhesion (7, 8). MDGA1 expression is also reported on primary cells and cell lines from leukemias, lymphomas, and tumors of the lung, colon, uterus, stomach and breast (3).

1. Litwack, E.D. et al. (2004) Mol. Cell. Neurosci. 25:263.
2. Diaz-Lopez, A. et al. (2005) Exp. Cell Res. 307:91.
3. De Juan, C. et al. (2002) Oncogene 21:3089.
4. Ishikawa, T. et al. (2011) Dev. Dyn. 240:96.
5. Takeuchi, A. and D.D.M. O'Leary (2006) J. Neurosci. 26:4460.
6. Takeuchi, A. et al. (2007) Cereb Cortex. 17:1531.
7. Fujimura, Y. et al. (2006) Brain Res. 1101:12.
8. Diaz-Lopez, A. et al. (2011) Cancer Microenviron. 4:23.

Bioinformatics

• Gene Symbol: Mdga1
• Uniprot:
  • NP_001074629()