ATPase Activity Assay Kit (Colorimetric)

ATPase Activity Assay Kit (Colorimetric) [NBP3-24475] - Standard Reference Curve

ATPase Activity Assay Kit (Colorimetric) [NBP3-24475] - Assay Example

• Reactivity: All-NA
• Applications: Func
• Suitable Sample Type: Animal tissue and cell samples
• Standard Curve Range: 0.06 - 2.75 U/L
• Sensitivity: 0.06 U/L

ATPase Activity Assay Kit (Colorimetric) Summary

• Description: Detection instrument: Microplate reader (630-640 nm, optimum wavelength: 640 nm)
  Assay time: 80 min
• Standard Curve Range: 0.06 - 2.75 U/L
• Sensitivity: 0.06 U/L
• Inter-Assay: %CV < 5
• Intra-Assay: %CV < 4
• Spike Recovery: Average recovery rate: 95%
• Kit Type: Activity Assay Kit (Colorimetric)
• Gene: DNAH8

Applications/Dilutions

• Dilutions:
  • Functional

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for ATPase Activity Assay Kit (Colorimetric)

• ATPase
• axonemal
• dynein, axonemal, heavy chain 8
• dynein, axonemal, heavy polypeptide 8
• FLJ25850
• FLJ36115
• FLJ36334
• hdhc9

Background

CATALYTIC ACTIVITY: ATP + H2O = ADP + phosphate. SUBCELLULAR LOCATION: Membrane; multi-pass membrane protein (By similarity). ALTERNATIVE PRODUCTS: 2 named isoforms produced by alternative splicing. SIMILARITY: Belongs to the cation transport ATPase (P-type) family. Type V subfamily.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

FAQs for ATPase Kit (NBP3-24475). (Showing 1 - 4 of 4 FAQ).

1. I have phosphate in my enzyme. What can I do?
   • You can dialyse or desalt the enzyme into a phosphate-free buffer. Alternatively, you can use a special resin (PiBind) to remove the phosphate.
2. I have 5% DMSO in my assay. Can I use PiColorLock Gold?
   • Yes, the reagent is designed for drug screening work and other situations that require DMSO.
3. I have a high background in my ATPase assay and I definitely do not have free phosphate in my sample
   • This is almost always due to inadequate mixing of the special stabilizer with the sample and detection reagent. Make sure the stabilizer is pipetted up and down several times to ensure thorough mixing.
4. I would like to measure the conversion of pyrophosphate to phosphate. Can I use the PiColorLock Gold Phosphate Detection System for this purpose?
   • Yes, only the phosphate will give a signal; pyrophosphate will not.

Bioinformatics

• Gene Symbol: DNAH8