Recombinant Mouse Mast Cell Protease-11/Prss34 Protein, CF


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Product Details

Reactivity MuSpecies Glossary
Applications Enzyme Activity

Order Details

Recombinant Mouse Mast Cell Protease-11/Prss34 Protein, CF Summary

Details of Functionality
Measured by its ability to cleave a colorimetric peptide substrate, N-carbobenzyloxy-Arg-ThioBenzyl ester (Z-R-SBzl), in the presence of 5,5’Dithio-bis (2-nitrobenzoic acid) (DTNB). Edwards, K.M. et al. (1999) J. Biol. Chem. 274:30468. The specific activity is >20,000 pmol/min/µg, as measured under the described conditions.
Mouse myeloma cell line, NS0-derived mouse Mast Cell Protease-11/Prss34 protein
Met20-Ser318, with a C-terminal 10-His tag
Accession #
N-terminal Sequence
Structure / Form
Pro form
Protein/Peptide Type
Recombinant Enzymes
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.


  • Enzyme Activity
Theoretical MW
34 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
48 kDa doublet, reducing conditions

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.
Supplied as a 0.2 μm filtered solution in MES and NaCl.
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Assay Procedure
  • Activation Buffer: 50 mM Tris, 0.15 M NaCl, 10 mM CaCl2, pH 7.5 (TCN)
  • Assay Buffer: 50 mM Tris, pH 8.0
  • Recombinant Mouse Mast Cell Protease‑11/Prss34 (rmMCP-11) (Catalog # 2857-SE)
  • Bacterial Thermolysin (Thermolysin) (Catalog # 3097-ZN)
  • 1,10 Phenanthroline (Sigma, Catalog # 320056), 0.6 M stock in DMSO
  • Substrate: Z-Arg-SBzl (SM Biochemicals, Catalog # SMSB01), 10 mM in DMSO
  • 5,5'Dithio-bis(2-nitrobenzoic acid) (DTNB) (Sigma, Catalog # D-8130)
  • 96-well Clear Plate (Costar, Catalog # 92592)
  • Plate reader (Model: SpectraMax Plus by Molecular Devices) or equivalent
  1. Dilute rmMCP-11 to 200 µg/mL with Activation Buffer.
  2. Dilute Thermolysin to 0.4 µg/mL with Activation Buffer.
  3. Mix equal volumes of 200 µg/mL rmMCP-11 and 0.4 µg/mL Thermolysin for final concentrations of 50 µg/mL and 0.2 µg/mL, respectively.
  4. Incubate at 37 °C for 30 minutes.
  5. Stop the reaction with 10 mM 1,10 Phenanthroline.
  6. Dilute activated rmMCP-11 to 0.1 ng/µL in Assay Buffer.
  7. Dilute substrate to 200 µM in Assay Buffer with 200 µM of DTNB.
  8. Load 50 µL of the 0.1 ng/µL rmMCP-11 into plate, and start the reaction by adding 50 µL of the substrate/DTNB mixture to wells. Include a Substrate Blank containing 50 µL Assay Buffer and 50 µL substrate mix without any rmMCP-11.
  9. Read in kinetic mode for 5 minutes at an absorbance of 405 nm.
  10. Calculate specific activity:

     Specific Activity (pmol/min/µg) =

Adjusted Vmax* (OD/min) x well volume (L) x 1012 pmol/mol
ext. coeff** (M-1cm-1) x path corr.*** (cm) x amount of enzyme (µg)

     *Adjusted for Substrate Blank
      **Using the extinction coefficient 13260 M-1cm-1
      ***Using the path correction 0.32 cm
     Note: the output of many spectrophotometers is in mOD Per Well:
  • rmMCP-11: 0.005 µg
  • DTNB: 100 µM
  • Substrate: 100 µM


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Mast Cell Protease-11/Prss34 Protein, CF

  • EOS
  • Mast Cell Protease11
  • Mast Cell Protease-11
  • MCP-11
  • MCPT11
  • Prss34


Mast Cell Protease-11 (MCP-11) is encoded by Prss34, one of 13 genes on mouse chromosome 17A3.3 that correspond to functional trypsin-like serine proteases (1). The deduced amino acid sequence of mouse MCP-11 consists of 318 residues with a signal peptide (residues 1 to 19), a pro region (residue 20 to 34), and a catalytic domain (35 to 318). The mRNA is preferentially expressed in spleen and bone marrow. The mouse MCP-11 (residues 20 to 318) was expressed in the NS0 cells with a foreign signal peptide. After being treated with thermolysin, the purified enzyme is active against a peptide substrate described in the Activity Assay Protocol. Apparently, the human gene corresponding to Prss34 encodes a protein that is not enzymatically active due to a mutation that leads to a premature translation termination codon.

  1. Wong, G.W. et al. (2004) J. Biol. Chem. 279:2438.

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Gene Symbol Prss34