Recombinant Mouse LRRTM3 Protein, CF

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse LRRTM3 Protein, CF Summary

• Details of Functionality: Measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons. Able to significantly enhance neurite outgrowth when immobilized as a 3 μL droplet containing 60-250 ng on a nitrocellulose coated microplate.
• Source: Mouse myeloma cell line, NS0-derived mouse LRRTM3 protein
  Met1-His419, with a C-terminal 6-His tag
• Accession #: Q8BZ81
• N-terminal Sequence: Glu31
• Protein/Peptide Type: Recombinant Proteins
• Gene: Lrrtm3
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 45.3 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 55-65 kDa, reducing conditons

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse LRRTM3 Protein, CF

• leucine rich repeat transmembrane neuronal 3
• leucine-rich repeat transmembrane neuronal protein 3
• LRRTM3
• MGC131810

Background

Leucine-rich repeat transmembrane neuronal 3 (LRRTM3) is a 68 kDa type I transmembrane protein that belongs to the LRRTM family, LRR superfamily of proteins (1). Mouse LRRTM3 is 582 amino acids (aa) in length with a 30 aa signal sequence, a 390 aa extracellular region, a 21 aa transmembrane region, and a 141 aa cytoplasmic domain. Within the extracellular region, there are ten LRRs flanked by cysteine-rich domains and one potential site for N‑linked glycosylation (1, 2). The cytoplasmic region contains several tyrosine, serine, and threonine residues that have potential to be phosphorylated and thus to be involved in signal transduction (1). There is also a conserved ECEV motif that interacts with PDZ proteins (1, 3). One isoform variant exists that shows an Ile514Val transition coupled to a deletion of aa 515 ‑ 582. Mature mouse LRRTM3 shares 98% aa sequence identity with mature human LRRTM3. All LRRTM proteins are expressed in the brain with varying profiles in other tissues (3). Strong LRRTM3 expression is present prenatally at 8.5 dpc in the neural progenitors of the anterior neural plate that will form the rostral neural tube (3). Expression is also seen in the region of the presomitic mesoderm/somite boundary plus the forebrain and developing eye (3). In the adult, LRRTM3 may be expressed both on neurons and placental trophoblasts. In neurons, it has been demonstrated to positively influenced by STOX1 and subsequently to induce BACE1 cleavage of APP, thus generating A beta PP beta production and a C99 fragment (2, 4).

1. Lauren, J. et al. (2003) Genomics 81:411.
2. Majercak, J. et al. (2006) Proc. Natl. Acad. Sci. USA 103:17967.
3. Haines, B.P. and P.W.J. Rigby (2007) Gene Expr. Patterns 7:23.
4. van Dijk, M. et al. (2010) J. Alzheimers Dis. 19:673.

Bioinformatics

• Gene Symbol: Lrrtm3
• Uniprot:
  • Q8BZ81()