Recombinant Mouse LILRB4/CD85k/ILT3 Protein, CF

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When Recombinant Mouse LILRB4/CD85k/ILT3 is coated onto a microplate at 2 µg/mL, Recombinant Human Angiopoietin-like Protein 7 (Catalog # 914-AN) binds with a typical ED50 of20-120 ng/mL.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Recombinant Mouse LILRB4/CD85k/ILT3 Protein, CF Summary

Details of Functionality
Measured by its binding ability in a functional ELISA. When Recombinant Mouse LILRB4/CD85k/ILT3 is coated onto a microplate at 2 μg/mL, Recombinant Human Angiopoietin-like Protein 7/ANGPTL7 (Catalog # 914-AN) binds with a typical ED50 = 20-120 ng/mL.
Source
Mouse myeloma cell line, NS0-derived mouse LILRB4/CD85k/ILT3 protein
Gly24-Lys238, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Gly24 & Ser37 (minor)
Protein/Peptide Type
Recombinant Proteins
Gene
Lilrb4a
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
25 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
34-42 kDa, reducing conditions

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse LILRB4/CD85k/ILT3 Protein, CF

  • CD85 antigen-like family member K
  • CD85k antigen
  • CD85k
  • HM18
  • ILT3
  • ILT-3
  • ILT3CD85K
  • leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains)
  • LILRB4
  • LIR5
  • LIR5LILRB5
  • LIR-5subfamily B, member 4
  • member 4

Background

LILRB4, also known as ILT3, CD85k, and LIR-5, is an approximately 60 kDa transmembrane glycoprotein that negatively regulates immune cell activation (1). Mature mouse LILRB4 consists of a 215 amino acid (aa) extracellular domain with two Ig-like domains, a 22 aa transmembrane segment, and a 75 aa cytoplasmic domain with 3 immunoreceptor tyrosine-based inhibitory motifs (ITIM) (2). Within the ECD, mouse LILRB4 shares 45% and 77% aa sequence identity with human and rat LILRB4, respectively. Alternative splicing of mouse LILRB4 generates a potentially soluble isoform that lacks the transmembrane segment (2). LILRB4 is expressed on dendritic cells (DC), monocytes, macrophages, and vascular endothelial cells (EC) (3, 6, 7). Ligation of LILRB4 triggers ITIM-mediated inhibition of cell-activating signaling, leading to enhanced immune tolerance and reduced allogeneic graft rejection (3, 5, 8, 9). Soluble LILRB4 induces the differentiation of CD8+ T suppressor cells (Ts) that can inhibit the effector functions of CD4+ Th cells and CD8+ CTL (5, 8, 10). In turn, CD8+ Ts cells induce LILRB4 up-regulation and a tolerogenic phenotype in monocytes, DC, and EC (6, 7, 9, 11, 12).
  1. Vlad, G. et al. (2010) Int. Rev. Immunol. 29:119.
  2. Castells, M.C. et al. (1994) J. Biol. Chem. 269:8393.
  3. Cella, M. et al. (1997) J. Exp. Med. 185:1743.
  4. Heinzmann, A. et al. (2000) Eur. J. Immunogenet. 27:121.
  5. Suciu-Foca, N. et al. (2007) J. Immunol. 178:7432.
  6. Gleissner, C.A. et al. (2007) Eur. J. Immunol. 37:177.
  7. Manavalan, J.S. et al. (2004) Int. Immunol. 16:1055.
  8. Vlad, G. and N. Suciu-Foca (2012) Exp. Mol. Pathol. 93:294.
  9. Chang, C.C. et al. (2002) Nat. Immunol. 3:237.
  10. Vlad, G. et al. (2006) Int. Immunopharmacol. 6:1889.
  11. Manavalan, J.S. et al. (2003) Transpl. Immunol. 11:245.
  12. Brenk, M. et al. (2009) J. Immunol. 183:145.

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Bioinformatics

Gene Symbol Lilrb4a
Uniprot