Recombinant Mouse LAIR1 Fc Chimera Protein, CF

When Bovine Collagen I is coated at 10 µg/mL, 100 μL/well, Recombinant Mouse LAIR1 Fc Chimera (Catalog # 10092-LR) binds with an ED50 of 0.2‑1.6 μg/mL

2 μg/lane of Recombinant Mouse LAIR1 Fc Chimera was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 55-62 kDa and 100-120 kDa, respectively.

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse LAIR1 Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. When Bovine Collagen I is coated at 10 µg/mL, 100 μL/well, Recombinant Mouse LAIR1 Fc Chimera binds with an ED₅₀ of 0.2-1.6 μg/mL.
• Source: Mouse myeloma cell line, NS0-derived mouse LAIR1 protein
  

  Mouse LAIR1 (Gln22-Tyr141) Accession # Q8BG84	IEGRMDP	Mouse IgG2a (Glu98-Lys330)
  N-terminus		C-terminus

• Accession #: Q8BG84
• N-terminal Sequence: No results obtained. Gln22 inferred from enzymatic pyroglutamate treatment revealing Glu23.
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 41 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 55-62 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse LAIR1 Fc Chimera Protein, CF

• CD305 antigen
• CD305
• CD305leukocyte-associated Ig-like receptor 1
• HLAIR1
• LAIR1
• LAIR-1
• leukocyte-associated immunoglobulin-like receptor 1

Background

Leukocyte-associated Ig-like receptor-1 (LAIR1) is an inhibitory receptor of the Ig superfamily that is structurally related to inhibitory members of KIR and ILT/CD85 families (1-3). It is expressed on immune cells, including NK cells, T cells, B cells, monocytes, immature neutrophils, dendritic cells and most thymocytes (2-4). The 253 amino acid (aa) type I transmembrane (TM) protein contains a 21 aa signal sequence, a 124 aa extracellular domain (ECD), a 20 aa TM domain and a 98 aa cytoplasmic domain. The ECD includes one C2-type Ig-like domain and two potential N-linked glycosylation sites. Tyrosine phosphorylation of two cytoplasmic ITIM motifs results in recruitment of phosphatases and down-regulation of signaling through activating receptors (2, 3, 5). Crosslinking of LAIR1 inhibits processes such as B cell receptor-mediated activation, NK cell and T cell cytotoxicity and basophil degranulation (1-3). Four mouse LAIR1 splice variants have been identified, but it is not known whether they are expressed as proteins (3). LAIR1b, which is the major alternate transcript, lacks most of the ECD. Of the minor transcripts, LAIR1c lacks a signal sequence, and LAIR1d and 1e lack a TM sequence. All mouse splice forms are identical in the last 90 aa of the cytoplasmic domain. LAIR1 shows high-affinity binding of collagens that results in inhibition of degranulation in a basophilic leukemia cell line (6). Human and mouse LAIR1 ECD share only 32% aa identity but, where studied, sites of expression and activity are similar (3-6). Mouse LAIR1 ECD also shares 62%, 31% and 28% aa identity with rat, canine, and bovine orthologs, respectively.

1. Meyaard, L. (2003) J. Biol. Regul. Homeost. Agents 17:330.
2. Meyaard, L. et al. (1997) Immunity 7:283.
3. Lebbink, R.J. et al. (2004) J. Immunol. 172:5535.
4. Verbrugge, A. et al. (2006) J. Leukoc. Biol. 79:828.
5. Verbrugge, A. et al. (2003) Int. Immunol. 15:1349.
6. Lebbink, R.J. et al. (2006) J. Exp. Med. 203:1419.

Bioinformatics

• Uniprot:
  • Q8BG84()