Recombinant Mouse Integrin alpha 5 beta 1 Protein, CF

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse Integrin alpha 5 beta 1 Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. When Bovine Fibronectin (Catalog # 1030-FN) is coated at 2 μg/mL, Recombinant Mouse Integrin alpha 5 beta 1 binds with an apparent K_D <0.5 nM.
• Source: Chinese Hamster Ovary cell line, CHO-derived mouse Integrin alpha 5 beta 1 protein
  

  Mouse Integrin alpha 5 Phe45-Asn999 (Gly377Ala) Accession # P11688	GS Linker	Acidic Tail	HHHHHH
  Mouse Integrin beta 1 (Gln21-Asp728) Accession # P09055	HP	GS Linker	Basic Tail
  N-terminus			C-terminus

• Accession #: P11688 (Integrin alpha 5) & P09055 (Integrin beta 1)
• N-terminal Sequence: Phe45 (Integrin alpha 5) & Gln21 predicted, No results obtained: sequencing might be blocked (Integrin beta 1)
• Structure / Form: Noncovalently-linked heterodimer
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 113 kDa (Integrin alpha 5) & 86.5 kDa (Integrin beta 1).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 115-165 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Integrin alpha 5 beta 1 Protein, CF

• CD49 antigen-like family member E
• CD49e
• Fibronectin receptor subunit alpha
• fibronectin receptor, alpha polypeptide
• fibronectin receptor, alpha subunit
• FNRA
• Integrin alpha 5 beta 1
• integrin alpha-5
• Integrin alpha-F
• integrin, alpha 5 (fibronectin receptor, alpha polypeptide)
• ITGA5/SLC9B1 fusion
• very late activation protein 5, alpha subunit
• VLA-5
• VLA5A

Background

Integrin alpha 5 beta 1, also known as VLA‑5, is a widely expressed non‑covalent heterodimer of a 160 kDa alpha 5 and a 130 kDa beta 1 Integrin subunit. alpha 5/ beta 1 functions in cell adhesion, migration, activation, and matrix fibrillogenesis (1, 2). Mature mouse Integrin  alpha 5/CD49e consists of a 955 aa extracellular domain (ECD) with 7 tandem FG‑GAP repeats, a 26 aa transmembrane segment, and a 28 aa cytoplasmic domain (3). Within the ECD, mouse alpha 5 shares 91% and 97% aa sequence identity with human and rat alpha 5, respectively. Mature mouse Integrin beta 1/CD29 consists of a 708 aa ECD with one vWF‑A domain and a cysteine‑rich region, followed by a 23 aa transmembrane segment and a 47 aa cytoplasmic domain (3). Within the ECD, mouse beta 1 shares 93% and 98% aa sequence identity with human and rat beta 1, respectively. alpha 5/ beta 1 binds Fibronectin in both RGD‑dependent and ‑independent manners (4, 5). It is up‑regulated on tumor vasculature and promotes angiogenesis (6, 7). This is accomplished in part by a constitutive association in cis of alpha 5/ beta 1 with VEGF R3 and Tie‑2, a requirement for optimal activation of those receptors (8, 9). alpha 5/ beta 1 interacts with a variety of other proteins, including ErbB2, uPAR, Galectin‑1, CTGF, and thrombin‑cleaved Osteopontin (5, 10‑13). alpha 5/ beta 1 also functions on some hematopoietic and neuronal stem cells (14‑16).

1. Mao, Y. and Schwarzbauer, J.E. (2005) Matrix Biol. 24:389.
2. Gu, J. and Taniguchi, N. (2004) Glycoconj. J. 21:9.
3. Holers, V.M. et al. (1989) J. Exp. Med. 169:1589.
4. Takagi, J. et al. (2003) EMBO J. 22:4607.
5. Wei, Y. et al. (2005) J. Cell Biol. 168:501.
6. Parsons-Wingerter, P. et al. (2005) Am. J. Pathol. 167:193.
7. Kim, S. et al. (2000) Am. J. Pathol. 156:1345.
8. Zhang, X. et al. (2004) J. Cell. Physiol. 202:205.
9. Cascone, I. et al. (2005) J. Cell Biol. 170:993.
10. Kuwada, S.K. et al. (2005) J. Biol. Chem. 280:19027.
11. Fischer, C. et al. (2005) J. Biol. Chem. 280:37266.
12. Gao, R. and Brigstock, D.R. (2005) Gastroenterology 129:1019.
13. Yokosaki, Y. et al. (2005) Matrix Biol. 24:418.
14. Carstanjen, D. et al. (2005) Transfusion 45:1192.
15. Gibson, R.M. et al. (2005) Mol. Cell. Neurosci. 28:588.
16. Tanaka, R. et al. (2009) J. Biol. Chem. 284:19817.

Publications for Integrin alpha 5 beta 1 (7728-A5)(4)

Publications using 7728-A5

• C Cegarra, B Cameron, C Chaves, T Dabdoubi, TM Do, B Genêt, V Roudières, Y Shi, P Tchepikoff, D Lesuisse An innovative strategy to identify new targets for delivering antibodies to the brain has led to the exploration of the integrin family PLoS ONE, 2022-09-15;17(9):e0274667. 2022-09-15 [PMID: 36108060] (ELISA Capture, Human)
• MS Tsai, MT Chiang, DL Tsai, CW Yang, HS Hou, YR Li, PC Chang, HH Lin, HY Chen, IS Hwang, PK Wei, CP Hsu, KI Lin, FT Liu, LY Chau Galectin-1 Restricts Vascular Smooth Muscle Cell Motility Via Modulating Adhesion Force and Focal Adhesion Dynamics Sci Rep, 2018-07-31;8(1):11497. 2018-07-31 [PMID: 30065303]
• Q Yan, SH Ahn, FM Medie, BK Sharma-Kui, LP Park, WK Scott, H Deshmukh, EL Tsalik, DD Cyr, CW Woods, CA Yu, C Adams, R Qi, B Hansen, VG Fowler Candidate genes on murine chromosome 8 are associated with susceptibility to Staphylococcus aureus infection in mice and are involved with Staphylococcus aureus septicemia in humans PLoS ONE, 2017-06-08;12(6):e0179033. 2017-06-08 [PMID: 28594911] (Bioassay, Mouse)
• Tseng C, Kolonin M Proteolytic Isoforms of SPARC Induce Adipose Stromal Cell Mobilization in Obesity. Stem Cells, 2015-09-22;34(1):174-90. 2015-09-22 [PMID: 26381424] (Bioassay)

Bioinformatics

• Uniprot:
  • P11688 (Integrin alpha 5) & P09055 (Integrin beta 1)()