Recombinant Mouse IL-36Ra/IL-1F5 Protein

• Reactivity: Mu
• Applications: Binding Activity, Bioactivity

Recombinant Mouse IL-36Ra/IL-1F5 Protein Summary

• Details of Functionality: Measured by its ability to inhibit IL-36 alpha , IL-36 beta or IL-36 gamma -induced IL-6 secretion by NIH‑3T3 mouse embryonic fibroblast cells. The ED₅₀ for this effect is 0.8-4 μg/mL in the presence of 15 ng/mL of Recombinant Mouse IL‑36 beta /IL‑1F8 (aa 31-183) (Catalog # 7060-ML). Measured by its binding ability in a functional ELISA. Immobilized Recombinant Mouse IL-36Ra/IL-1F5 at 1 µg/mL (100 µL/well) can bind Recombinant Human IL-1 Rrp2/IL-1 R6 Fc Chimera(Catalog # 872-RP) with a linear range of 0.15-5 µg/mL.
• Source: E. coli-derived mouse IL-36Ra/IL-1F5 protein
  Val2-Asp156
• Accession #: Q9QYY1
• N-terminal Sequence: Val2
• Protein/Peptide Type: Recombinant Proteins
• Gene: IL36RN
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Endotoxin Note: <0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Binding Activity2
  • Bioactivity
• Theoretical MW: 17 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS and DTT with BSA as a carrier protein.
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution Instructions: Reconstitute at 250 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse IL-36Ra/IL-1F5 Protein

• FIL1 delta
• FIL1
• FIL1-delta, IL-1RP3, IL-1L1, IL-1-delta)
• FIL1DIL-1 delta
• IL1F5 (Canonical product IL-1F5a)
• IL1F5
• IL-1HY1
• IL-1L1
• IL-1RP3
• IL36Ra
• IL-36Ra
• IL36RN
• interleukin 1 family, member 5 (delta)
• interleukin 1, delta
• Interleukin 36 Receptor Antagonist
• interleukin-1 family member 5
• Interleukin-1 HY1
• Interleukin-1 receptor antagonist homolog 1
• Interleukin-1-like protein 1
• Interleukin-36 Receptor Antagonist

Background

Mouse interleukin-36 receptor antagonist (IL-36Ra; previously IL-1F5; also named FIL-1δ [delta], IL‑1HY1, IL-1H3, and IL-1L1) is a member of the IL‑1 family of proteins (1-6). IL‑1 family members include IL-1 beta , IL-1 alpha , IL-1ra, IL‑18 and IL‑1F5 through F10 (6, 7). All family members show a 12 beta -strand, beta -trefoil configuration, and are believed to have arisen from a common ancestral gene that underwent multiple duplications (8). The mouse IL‑36Ra/IL‑1F5 gene maps to a region on mouse chromosome 2 that contains all other IL-1 family members (except IL‑18), supporting an evolutionary relationship with the IL-1 family (1, 9). It is particularly close to the gene for IL-1ra and is likely a relatively recent duplication of that gene. IL‑36Ra/IL‑1F5 is synthesized as a 156 amino acid (aa) protein that contains no signal sequence, no prosegment and no potential N-linked glycosylation site(s) (2, 5, 8). Nevertheless, it appears to be secreted as a 17 kDa monomer. In humans, there is an alternate start site that potentially gives rise to an alternate splice form (5). This translated product has a premature stop codon, resulting in a truncated 16 aa peptide. Mouse to human, full length IL‑36Ra/IL‑1F5 has 90% aa identity. Within the family, IL-36Ra/IL-1F5 is 48%, 30%, 35%, 35%, 35%, 37% and 43% aa identical to IL‑1ra, IL‑1 beta , IL‑36 alpha /IL‑1F6, IL‑37/IL‑1F7, IL-36 beta /IL-1F8, IL‑36 gamma /IL‑1F9 and IL‑1F10, respectively. Cells reported to express IL-36Ra/IL-1F5 include monocytes, B cells, dendritic cells/Langerhans cells, keratinocytes, and gastric fundus Parietal and Chief cells (1, 8). The receptor for IL-36Ra/IL-1F5 has not been positively identified. Indirect evidence suggests it is IL‑1 Rrp2 and/or IL-1 RAcP (9). In either case, activity association with receptor binding is unclear. It was initially reported to be an antagonist of  IL-36 gamma activity (4, 7). This would be consistent with its hypothesized relationship to IL‑1ra.

1. Smith, D. E. et al. (2000) J. Biol. Chem. 275:1169.
2. Kumar, S. et al. (2000) J. Biol. Chem. 275:10308.
3. Mulero, J.J. et al. (1999) Biochem. Biophys. Res. Commun. 263:702.
4. Nicklin, M.J.H. et al. (2002) Genomics. 79:718.
5. Barton J. L. et al. (2000) Eur. J. Immunol. 30:3299.
6. Dinarello, C. et al. (2010) Nat. Immunol. 11:973.
7. Dunn, E. et al. (2001) Trends Immunol. 22:533.
8. Debets, R. et al. (2001) J. Immunol. 167:1440.
9. Towne, J.E. et al. (2004) J. Biol. Chem. 279:13677.

Publications for IL-36Ra/IL-1F5 (2714-ML)(4)

Publications using 2714-ML

• Chen, Q;Gao, N;Yu, FS; Interleukin-36 Receptor Signaling Attenuates Epithelial Wound Healing in C57BL/6 Mouse Corneas Cells 2023-06-08 [PMID: 37371057] (In Vivo, Mouse)
• E Giannoudak, YE Hernandez-, K Mulfaul, SL Doyle, E Hams, PG Fallon, A Mat, D O'Shea, M Kopf, AE Hogan, PT Walsh Interleukin-36 cytokines alter the intestinal microbiome and can protect against obesity and metabolic dysfunction Nat Commun, 2019-09-05;10(1):4003. 2019-09-05 [PMID: 31488830] (In Vivo, Mouse)
• N Gao, R Me, C Dai, B Seyoum, FX Yu Opposing Effects of IL-1Ra and IL-36Ra on Innate Immune Response to Pseudomonas aeruginosa Infection in C57BL/6 Mouse Corneas J. Immunol., 2018-06-11;0(0):. 2018-06-11 [PMID: 29891552] (In Vivo, Mouse)
• Scheiermann P, Bachmann M, Hardle L, Pleli T, Piiper A, Zwissler B, Pfeilschifter J, Muhl H Application of IL-36 receptor antagonist weakens CCL20 expression and impairs recovery in the late phase of murine acetaminophen-induced liver injury. Sci Rep, 2015-02-17;5(0):8521. 2015-02-17 [PMID: 25687687] (In Vivo, Mouse)

Bioinformatics

• Gene Symbol: IL36RN
• Uniprot:
  • Q9QYY1()