Recombinant Mouse IL-36 beta/IL-1F8 Protein

• Reactivity: Mu
• Applications: Binding Activity

Recombinant Mouse IL-36 beta/IL-1F8 Protein Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. Immobilized recombinant mouse IL-36 beta at 1 µg/mL (100 µL/well) can bind recombinant mouse IL-1 Rrp2 (Catalog # 2354-RP) with a linear range of 0.15 ‑ 5 µg/mL.
• Source: E. coli-derived mouse IL-36 beta/IL-1F8 protein
  Met1-Lys183
• Accession #: Q9D6Z6
• N-terminal Sequence: Met1
• Protein/Peptide Type: Recombinant Proteins
• Gene: Il1f8
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Binding Activity
• Theoretical MW: 21 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse IL-36 beta/IL-1F8 Protein

• family of interleukin 1-eta
• FIL1 eta
• FIL1
• FIL1-(ETA)
• FIL1H
• FILI-(ETA)
• IL-1 eta
• IL1-ETA
• IL1F8 (Canonical product IL-1F8a)
• IL-1F8 (FIL1-eta)
• IL1F8
• IL-1F8
• IL1H2
• IL-1H2
• IL36 beta
• IL-36 beta
• IL36B
• interleukin 1 family, member 8 (eta)
• interleukin 1, eta
• Interleukin 36, Beta
• Interleukin-1 eta
• interleukin-1 family member 8
• Interleukin-1 homolog 2
• Interleukin-1 Superfamily e
• Interleukin-36 Beta

Background

Mouse interleukin‑36 beta [IL‑36 beta ; previously IL‑1F8 and also named FIL‑1 eta (eta) and IL‑1H2] is a member of the IL‑1 family of proteins (1 ‑ 3, 6). IL‑1 family members include IL‑1 beta , IL‑1 alpha , IL‑1ra, IL‑18, IL‑36Ra/IL‑1F5, IL‑36 alpha /IL‑1F6, IL‑37/IL‑1F7, IL‑36 gamma /IL‑1F9 and IL‑1F10 (4, 6). All family members show a 12 beta ‑stranded beta ‑trefoil configuration, and are believed to have arisen from a common ancestral gene that has undergone multiple duplications (4). Although two alternatively spliced transcript variants for human IL‑36 beta /IL‑1F8 have been described, to date, only one mouse IL‑36 beta /IL‑1F8 isoform is known (3). Mouse IL‑36 beta /IL‑1F8 is synthesized as a 183 amino acid (aa) protein that contains no signal sequence, no prosegment and no potential N‑linked glycosylation site(s) (1, 2). It is likely that mouse IL‑36 beta /IL‑1F8, similarly to its human homologue, is actively secreted (1). Mouse IL‑36 beta /IL‑1F8 shares 61% and 74% aa identity with human IL‑36 beta /IL‑1F8 isoform 2 and rat IL‑1F8, respectively. Within the IL‑1 family, IL‑36 beta /IL‑1F8 shares 27% sequence with 33%, 32%, 35%, 39% and 28% aa sequence identity with IL‑1ra, IL‑1 beta , IL‑36Ra/IL‑1F5, IL‑36 alpha /IL‑1F6, IL‑36 gamma /IL‑1F9 and IL‑1F10, respectively. Cells reported to express IL‑36 beta /IL‑1F8 include resting and activated monocytes and B cells (1, 4). The receptor for IL‑36 beta /IL‑1F8 is reported to be a combination of IL‑1 Rrp2 and IL‑1 RAcP (5). Recombinant IL‑36 beta /IL‑1F8, along with IL‑36 alpha /IL‑1F6 and IL‑36 gamma /IL‑1F9, has been shown to activate the pathway involving NF‑ kappa B and MAPK in an IL‑1 Rrp2 dependent manner.

1. Smith, D.E. et al. (2000) J. Biol. Chem. 275:1169.
2. Kumar, S. et al. (2000) J. Biol. Chem. 275:10308.
3. Nicklin, M.J.H. et al. (2002) Genomics. 79:718.
4. Dunn, E. et al. (2001) Trends Immunol. 22:533.
5. Towne, J.E. et al. (2004) J. Biol. Chem. 279:13677.
6. Dinarello, C. et al. (2010) Nat. Immunol. 11:973.

Publications for IL-36 beta/IL-1F8 (2298-ML)(2)

Publications using 2298-ML

• C Morales De, JR Maxwell, MM Xu, A Menoret, P Mittal, N Tsurutani, AJ Adler, AT Vella Costimulation Induces CD4�T Cell Antitumor Immunity via an Innate-like Mechanism Cell Rep, 2019-04-30;27(5):1434-1445.e3. 2019-04-30 [PMID: 31042471] (Bioassay, Mouse)
• Magne D, Palmer G, Barton JL, Mezin F, Talabot-Ayer D, Bas S, Duffy T, Noger M, Guerne PA, Nicklin MJ, Gabay C The new IL-1 family member IL-1F8 stimulates production of inflammatory mediators by synovial fibroblasts and articular chondrocytes. Arthritis Res. Ther., 2006-04-28;8(3):R80. 2006-04-28 [PMID: 16646978] (ELISA (Standard))

Bioinformatics

• Gene Symbol: Il1f8
• Uniprot:
  • Q9D6Z6()